Systemic administration of cholera toxin B subunit conjugated to horseradish peroxidase in the adult rat labels preganglionic autonomic neurons, motoneurons, and select primary afferents for light and electron microscopic studies

Journal of Neuroscience Methods

Volumn 149, Issue 2, 2005, Pages 101-109

  Havton, Leif A ^a   Broman, Jonas ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LUND UNIVERSITY   (Sweden)

Author keywords

GABA; Immunogold; Intraperitoneal; Visceral

Indexed keywords

AMINO ACID RECEPTOR AFFECTING AGENT; CHOLERA TOXIN B SUBUNIT; HORSERADISH PEROXIDASE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; AUTONOMIC NERVE; CELL SELECTION; CONJUGATION; ELECTRON MICROSCOPY; FEMALE; IMMUNOGOLD LABELING; MOTONEURON; MUSCLE SPINDLE AFFERENT NERVE; NERVE PROJECTION; NONHUMAN; PREGANGLIONIC NERVE CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; RAT; SYNAPSE;

ANIMALS; AUTONOMIC FIBERS, PREGANGLIONIC; CHOLERA TOXIN; FEMALE; HORSERADISH PEROXIDASE; INJECTIONS, INTRAPERITONEAL; MICROSCOPY, ELECTRON, TRANSMISSION; MOTOR NEURONS; NEURAL PATHWAYS; NEURONS, AFFERENT; RATS; RATS, SPRAGUE-DAWLEY; STAINING AND LABELING;

EID: 27844463520     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2005.03.014     Document Type: Article

References (33)

1. J.M. Alisky, C.I. van de Wetering, and B.L. Davidson Widespread dispersal of cholera toxin subunit b to brain and spinal cord neurons following systemic delivery Exp Neurol 178 2002 139 146
2. R. Ambalavanar, and R. Morris Fluoro-gold injected either subcutaneously or intravascularly results in extensive retrograde labeling of CNS neurons having axons terminating outside the blood-brain barrier Brain Res 505 1989 171 175
3. C.R. Anderson NADPH diaphorase-positive neurons in the rat spinal cord include a subpopulation of autonomic preganglionic neurons Neurosci Lett 139 1992 280 284
4. J. Broman, S. Anderson, and O.P. Ottersen Enrichment of glutamate-like immunoreactivity in primary afferent terminals throughout the spinal cord dorsal horn Eur J Neurosci 5 1993 1050 1061
5. L.I. Bruijn, T.M. Miller, and D.W. Cleveland Unraveling the mechanisms involved in motor neuron degeneration in ALS 27 2004 723 749
6. G. Grant Projection patterns of primary sensory neurons studied by transganglionic methods: somatotopy and target-related organization Brain Res Bull 30 1993 199 208
7. M.E. Gurney, H. Pu, A.Y. Chiu, M.C. Del Canto, C.Y. Polchow, and D.D. Alexander Motor neuron degeneration in mice that express a human Cu, Zn superoxide dismutase mutation Science 264 1994 1772 1775
8. M.A. Henry, L.E. Westrum, and L.R. Johnson Ultrastructure of transganglionic HRP transport in cat trigeminal system Brain Res 334 1985 255 266
9. Y. Hosoya, I. Nadelhaft, D. Wang, and K. Kohno Thoracolumbar sympathetic preganglionic neurons in the dorsal commissural nucleus of the male rat: an immunohistochemical study using retrograde labeling of cholera toxin subunit B. Exp Brain Res 98 1994 21 30
10. T.X. Hoang, J.H. Nieto, N.J.K. Tillakaratne, and L.A. Havton Autonomic and motor neuron death is progressive and parallel in a lumbosacral ventral root avulsion model of cauda equina injury J Comp Neurol 467 2003 477 486
11. C. Köbbert, R. Apps, I. Bechmann, J.L. Lanciego, J. Mey, and S. Thanos Current concepts in neuroanatomical tracing Prog Neurobiol 62 2000 327 351
12. M. Larsson, S. Persson, O.P. Ottersen, and J. Broman Quantitative analysis of immunogold labeling indicates low levels and non-vesicular localization of l-aspartate in rat primary afferent terminals J Comp Neurol 430 2001 147 159
13. S.K. Leong, and E.A. Ling Labeling neurons with fluorescent dyes administered via intravenous, subcutaneous or intraperitoneal route J Neurosci Meth 32 1990 15 24
14. A. Levinsson, H. Holmberg, J. Broman, M. Zhang, and J. Schouenborg Spinal sensorimotor transformation: relation between cutaneous somatotopy and a reflex network J Neurosci 22 2002 8170 8182
15. T. Mannen, M. Iwata, Y. Toyokura, and K. Nagashima The Onuf's nucleus and the external anal sphincter muscles in amyotrophic lateral sclerosis and Shy-Drager syndrome Acta Neuropathol 58 1982 255 260
16. M.M. Mesulam Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents J Histochem Cytochem 26 1978 106 117
17. M.M. Mesulam, E. Hegarty, H. Barbas, K.A. Carson, E.C. Gower, and A.G. Knapp Additional factors influencing sensitivity in the tetramethyl benzidine method for horseradish peroxidase neurohistochemistry J Histochem Cytochem 28 1980 1255 1259
18. S. Nicole, C. Cifuentes Diaz, T. Frugier, and J. Melki Spinal muscular atrophy: recent advances and future prospects Muscle Nerve 26 2002 4 13
19. L. Novikova, L. Novikov, and J.-O. Kellerth Persistent neuronal labeling by retrograde fluorescent tracers: a comparison between Fast Blue Fluoro-Gold and various dextran conjugates J Neurosci Meth 74 1997 9 15
20. F. Olucha, F. Martinez-Garcia, and C. Lopez-Garcia A new stabilizing agent for the tetramethyl benzidine (TMB) reaction product in the histochemical detection of horseradish peroxidase (HRP) J Neurosci Meth 13 1985 131 138
21. O.P. Ottersen Postembedding light- and electron microscopic immunocytochemistry of amino acids: description of a new model system allowing identical conditions for specificity testing and tissue processing Exp Brain Res 69 1987 167 174
22. D.V. Pow, and D.K. Crook Extremely high titre polyclonal antisera against small neurotransmitter molecules: rapid production, characterisation and use in light- and electron-microscopic immunocytochemistry J Neurosci Meth 48 1993 51 63
23. H.J. Ralston Analysis of neuronal networks: a review of techniques for labeling axonal projections J Electr Microsc Tech 15 1990 322 331
24. F.J. Richmond, Gladdy, J.L. Creasy, S. Kitamura, E. Smits, and D.B. Thomson Efficacy of seven retrograde tracers compared in multiple-labelling studies of feline motoneurons J Neurosci Meth 53 1994 35 46
25. Rivero-Melian, C. Organization of hindlimb nerve projections to the rat spinal cord: A choleragenoid horseradish peroxidase study. J Comp Neurol 1996;364:651-63.
26. B. Robertson, and J. Arvidsson Transganglionic transport of wheat germ agglutinin-HRP and choleragenoid-HRP in rat trigeminal primary sensory neurons Brain Res 348 1985 44 51
27. B. Robertson, and G. Grant A comparison between wheat germ agglutinin and choleragenoid-horseradish peroxidase as anterogradely transported markers in central branches of primary sensory neurones in the rat with some observations in the cat Neuroscience 14 1985 895 905
28. Y. Sugiura, N. Terui, and Y. Hosoya Difference in distribution of central terminals between visceral and somatic unmyelinated (C) primary afferent fibers J Neurophysiol 62 1989 834 840
29. B.A. Svensson, J. Rastad, and J. Westman Endogenous peroxidase-like activity in the feline dorsal column nuclei and spinal cord Exp Brain Res 55 1984 325 332
30. V.G. VanderHorst, and G. Holstege Organization of lumbosacral motoneuronal cell groups innervating hindlimb, pelvic floor, and axial muscles in the cat J Comp Neurol 382 1997 46 76
31. V.G. VanderHorst, E. Terasawa, H.J. Ralston, and G. Holstege Monosynaptic projections from the nucleus retroambiguus to motoneurons supplying the abdominal wall, axial, hindlimb, and pelvic floor muscles in the female rhesus monkey J Comp Neurol 424 2000 233 250
32. H.F. Wang, P. Shortland, M.J. Park, and G. Grant Retrograde and transganglionic transport of horseradish peroxidase-conjugated cholera toxin B subunit, wheatgerm agglutinin and isolectin B4 from Griffonia simplicifolia I in primary afferent neurons innervating the rat urinary bladder Neuroscience 87 1998 275 288
33. G.K. Wenning, C. Colosimo, F. Geser, and W. Poewe Multiple system atrophy Lancet Neurol 3 2004 93 103