An improved method for detection and quantification of chitinase activities

Canadian Journal of Microbiology

Volumn 51, Issue 6, 2005, Pages 491-495

  Guthrie, Jennifer L ^a   Khalif, Sagal ^a   Castle, Alan J ^a  

^a BROCK UNIVERSITY   (Canada)

Author keywords

Chitinases; Electrophoresis; Non denaturing gels

Indexed keywords

BACTERIA; ELECTROPHORESIS; FUNGI; GELS; GROWTH KINETICS; OLIGOMERS; PLANTS (BOTANY); POLYACRYLATES;

CHITINASES; FUNGAL GROWTH; FUNGAL PATHOGENS; NON-DENATURING GELS;

ENZYMES;

ACETYLGLUCOSAMINIDASE; CHITIN; CHITINASE; CHITOBIOSIDASE; DISACCHARIDE; ENDOCHITINASE; N ACETYLGLUCOSAMINE; OLIGOSACCHARIDE; POLYMER; UNCLASSIFIED DRUG;

DETECTION METHOD; ENZYME;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FUNGAL STRAIN; FUNGUS GROWTH; NONHUMAN; PATHOGENESIS; PLANT; PLANT DISEASE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DENATURATION; QUANTITATIVE ANALYSIS;

AGARICUS; CHITINASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYMECROMONE; SUBSTRATE SPECIFICITY; TRICHODERMA;

FUNGI; HEXAPODA;

EID: 27844461471     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w05-020     Document Type: Article

References (15)

1. Bradford, M.M. 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem. 72: 248-254.
2. Carsolio, C., Gutiérrez, A., Jiménez, B., Van Montagu, M., and Herrera-Estrella, A. 1994. Characterization of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism. Proc. Natl. Acad. Sci. USA, 91: 10903-10907.
3. Cohen-Kupiec, R., and Chet, I. 1998. The molecular biology of chitin digestion. Curr. Opin. Biotechnol. 9: 270-277.
4. De la Cruz, J., Hidalgo-Gallego, A., Lora, J.M., Benitez, T., Pintor-Toro, J.A., and Llobell, A. 1992. Isolation and characterization of three chitinases from Trichoderma harzianum. Eur. J. Biochem. 206: 859-867.
5. Donzelli, B.G.G., and Harman, G.E. 2001. Interaction of ammonium, glucose, and chitin regulates the expression of cell wall-degrading enzymes in Trichoderma atroviride strain P1. Appl. Environ. Microbiol. 67: 5643-5647.
6. Haran, S., Schickler, H., Oppenheim, A., and Chet, I. 1995. New components of the chitinolytic system of Trichoderma harzianum. Mycol. Res. 99: 441-446.
7. Harman, G.E., Hayes, C.K., Lorito, M., Broadway, R.M., Di Pietro, A. Peterbauer, C., and Trönsmo, A. 1993. Chitinolytic enzymes of Trichoderma harzianum: purification of chitobiosidase and endochitinase. Phytopathology, 83: 313-318.
8. Lorito, M., Hayes, C.K., Di Pietro, A., Woo, S.L., and Harman, G.E. 1994. Purification, characterization, and synergistic activity of a glucan 1,3-β-glucosidase and an N-acetyl-β-glucosaminidase from Trichoderma harzianum. Phytopathology, 84: 398-405.
9. Omero, C., Horwitz, B.A., and Chet, I. 2001. A convenient fluorometric method for the detection of extracellular N-acetylglucosaminidase production by filamentous fungi. J. Microbiol. Methods, 43: 165-169.
10. Raper, C.A., Raper, J.R., and Miller, R.E. 1972. Genetic analysis of the life cycle of Agaricus bisporus. Mycologia, 64: 1088-1117.
11. Sahai, A.S., and Manocha, M.S. 1993. Chitinases of fungi and plants: their involvement in morphogenesis and host-parasite interaction. FEMS Microbiol. Rev. 11: 317-338.
12. Samuels, G.J., Dodd, S.L., Gams, W., Castlebury, L.A., and Petrini, O. 2002. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia, 94: 146-170.
13. Trönsmo, A., and Harman, G.E. 1993. Detection and quantification of N-acetyl-β-D-glucosaminidase, chitobiosidase, and endochitinase in solutions and on gels. Anal. Biochem. 208: 74-79.
14. Ulhoa, C., and Peberdy, J. 1991. Purification and characterization of an extracellular chitobiase from Trichoderma harzianum. Curr. Microbiol. 23: 285-289.
15. Ulhoa, C., and Peberdy, J. 1992. Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum. Enzyme Microb. Technol. 14: 236-240.