Easy and rapid method for the determination of gene expression in cumulus cells incubated for oocyte maturation

Reproductive Biology and Endocrinology

Volumn 3, Issue , 2005, Pages

  Kumamoto, Kanako ^a   Wang, Haifeng ^a   Yamashiro, Hideaki ^a   Terada, Takato ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; DEOXYRIBONUCLEASE I; GENOMIC DNA; MAGNESIUM ION; PRIMER DNA; RIBOSOME RNA; RNA; SOMATOMEDIN C; MAGNESIUM;

ACCURACY; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL COUNT; CELL LYSATE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CUMULUS OOPHORUS; CYTOLYSIS; FEMALE; GENE AMPLIFICATION; GENE EXPRESSION; GENE TARGETING; INCUBATION TIME; METHODOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; OOCYTE MATURATION; PLASMID; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA PURIFICATION; SAMPLING; STANDARD; TEMPERATURE MEASUREMENT; ANIMAL; BIOSYNTHESIS; CATTLE; CYTOLOGY; METABOLISM; OOCYTE; PHYSIOLOGY; POLYMERASE CHAIN REACTION;

ANIMALIA;

ANIMALS; CATTLE; DEOXYRIBONUCLEASE I; FEMALE; GENE EXPRESSION; INSULIN-LIKE GROWTH FACTOR I; MAGNESIUM; OOCYTES; POLYMERASE CHAIN REACTION;

EID: 27744608202     PISSN: 14777827     EISSN: 14777827     Source Type: Journal    
DOI: 10.1186/1477-7827-3-59     Document Type: Article

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