Quantitative detection of enteroviruses in activated sludge by cell culture and real-time RT-PCR using paramagnetic capturing

Journal of Water and Health

Volumn 3, Issue 3, 2005, Pages 313-324

  Pusch, D ^a   Ihle, St ^a   Lebuhn, M ^b   Graeber, I ^a   Lopez Pila J M ^a  

^a UMWELTBUNDESAMT   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Activated sludge; Dynabeads capturing; Enteroviruses; Poliovirus sabin 1; Quantitative real time RT PCR

Indexed keywords

POLYSTYRENE DERIVATIVE;

ARTICLE; CULTURE TECHNIQUE; DNA SEQUENCE; ELECTRON SPIN RESONANCE; ENTEROVIRUS; EVALUATION; GERMANY; ISOLATION AND PURIFICATION; METHODOLOGY; OLIGONUCLEOTIDE PROBE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEWAGE; VIROLOGY;

CELL CULTURE TECHNIQUES; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENTEROVIRUS; EVALUATION STUDIES; GERMANY; OLIGONUCLEOTIDE PROBES; POLYSTYRENES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SEWAGE;

EID: 27744607125     PISSN: 14778920     EISSN: None     Source Type: Journal    
DOI: 10.2166/wh.2005.039     Document Type: Article

References (16)

1. Becker, S., Böger, P., Oehlmann, R. & Ernst, A. 2000 PCR bias in ecological analysis: a case study for quantitative Taq nuclease assays in analyses of microbial communities. Appl. Environ. Microbiol. 66, 4945-4953.
2. Borchardt, M. A., Bertz, P. D., Spencer, S. K. & Battigelli, D. A. 2003 Incidence of enteric viruses in groundwater from household wells in Wisconsin. Appl. Environ. Microbiol. 69(2), 1172-1180. (Pubitemid 36204849)
3. Enriquez, C. E., Abbaszadegan, M., Pepper, I. L., Richardson, K. J., Margolin, A. B. & Gerba, Ch. P. 1993 Comparison of poliovirus detection in water by cell culture and nucleic acid hybridisation. Wat. Sci. Technol. 27, 315-319. (Pubitemid 23160911)
4. Fuchs, B. M., Syutsubo, K., Ludwig, W. & Amann, R. 2001 In situ accessibility of Escherichia coli 23S rRNA to fluorescently labelled oligonucleotide probes. Appl. Environ. Microbiol. 67, 961-968. (Pubitemid 32121394)
5. Gilgen, M., Wegmüller, B., Burkhalter, P., Bühler, H. P., Müller, U., Lüthy, J. & Candrian, U. 1995 Reverse transcription PCR to detect enteroviruses in surface water. Appl. Environ. Microbiol. 61, 1226-1231.
6. Holland, P. M., Abramson, R. D., Watson, R. & Gelfland, D. H. 1991 Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl Acad. Sci. USA 88, 7276-7280. (Pubitemid 21915133)
7. Jacobsen, C. 1995 Microscale detection of specific bacterial DNA in soil with a magnetic capture-hybridization and PCR amplification assay. Appl. Environ. Microbiol. 61, 3347-3352.
8. Kopecka, H., Dubrou, S., Prevot, J., Marechal, J. & López-Pila, J. M. 1993 Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridisation. Appl. Environ. Microbiol. 59, 1-7.
9. Lebuhn, M., Effenberger, M., Gronauer, A. & Wilderer, P. 2003 Using quantitative real-time PCR to determine the hygienic status of cattle manure. Wat. Sci. Technol. 48(4), 97-103. (Pubitemid 37180072)
10. Loisy, F., Le Cann, P., Pommepuy, M. & Le Guyader, F. 2000 An improved method for the detection of Norwalk-like caliciviruses in environmental samples. Lett. Appl. Microbiol. 31(6), 411-415. (Pubitemid 32059882)
11. Maher, N., Dillon, H. K., Vermund, S. H. & Unnasch, T. R. 2001 Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA. Appl. Environ. Microbiol. 67, 449-452. (Pubitemid 32044449)
12. Margolin, A. B., Gerba, Ch. P., Richardson, K. J. & Naranjo, J. E. 1993 Comparison of cell culture and a poliovirus gene probe assay for the detection of enteroviruses in environmental water samples. Wat. Sci. Technol. 27, 311-314. (Pubitemid 23160910)
13. Monpoeho, S., Dehée, A., Mignotte, B., Schwartzbrod, L., Marechal, V., Nicolas, J.-C., Billaudel, S. & Férré, V. 2000 Quantification of enterovirus RNA in sludge samples using single tube real-time RT-PCR. BioTechniques 29, 88-93. (Pubitemid 30439421)
14. Regan, P. M. & Margolin, A. B. 1997 Development of a nucleic acid capture probe with reverse transcriptase-polymerase chain reaction to detect poliovirus in groundwater. J. Virol. Methods 64, 65-72. (Pubitemid 27077038)
15. Schaffer, F. L. & Schwerdt, C. E. 1959 Purification and properties of poliovirus. Adv. Virus Res. 6, 195-204.
16. Schvoerer, E., Ventura, M., Dubos, O., Cazaux, G., Serceau, R., Gournier, N., Dubois, V., Caminade, P., Fleury, H. J. A. & Lafon, M. 2001 Qualitative and quantitative molecular detection of enteroviruses in water from bathing areas and from a sewage treatment plant. Res. Microbiol. 152, 179-186. (Pubitemid 32222964)