The cryofixation of isolated rat gastric mucosa provides new insights into the functional transformation of gastric parietal cells: An in vitro experimental model study

Archives of Histology and Cytology

Volumn 68, Issue 3, 2005, Pages 151-160

  Sawaguchi, Akira ^a   Aoyama, Fumiyo ^a   Ide, Soyuki ^a   Suganuma, Tatsuo ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CIMETIDINE; EZRIN; HISTAMINE; HYDROGEN POTASSIUM ADENOSINE TRIPHOSPHATASE;

ANIMAL CELL; ANIMAL TISSUE; APICAL MEMBRANE; ARTICLE; CELL STIMULATION; CELL STRUCTURE; CELL TRANSFORMATION; CRYOFIXATION; CYTOPLASM; ELECTRON MICROSCOPY; EXPERIMENTAL MODEL; FREEZING; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MALE; MICROSCOPY; MICROVILLUS; NONHUMAN; RAT; STIMULATION; STOMACH ACID SECRETION; STOMACH MUCOSA; STOMACH PARIETAL CELL; TIME; TISSUE FIXATION;

ANIMALS; CIMETIDINE; CRYOPRESERVATION; CYTOSKELETAL PROTEINS; GASTRIC MUCOSA; HISTAMINE; IMMUNOHISTOCHEMISTRY; MALE; MICROSCOPY, ELECTRON; PARIETAL CELLS, GASTRIC; PHOSPHOPROTEINS; RATS; RATS, WISTAR;

EID: 27744596421     PISSN: 09149465     EISSN: 13491717     Source Type: Journal    
DOI: 10.1679/aohc.68.151     Document Type: Article

References (25)

1. Agnew BJ, Duman JG, Watson CL, Coling DE, Forte JG: Cytological transformations associated with parietal cell stimulation: critical steps in the activation cascade. J Cell Sci 112: 2639-2646 (1999).
2. Berglindh T, Öbrink KJ: A method for preparing isolated glands from the rabbit gastric mucosa. Acta Physiol Scand 96: 150-159 (1976).
3. Chew CS, Ljungstrom M, Smolka A, Brown MR: Primary culture of secretagogue-responsive parietal cells from rabbit gastric mucosa. Am J Physiol 256: G254-G263 (1989).
4. DiBona DR, Ito S, Berglindh T, Sachs G: Cellular site of gastric acid secretion. Proc Natl Acad Sci USA 76: 6689-6693 (1979).
5. Duman JG, Pathak NJ, Ladinsky MS, McDonald KL, Forte JG: Three-dimensional reconstruction of cytoplasmic membrane networks in parietal cells. J Cell Sci 115: 1251-1258 (2002).
6. Forte JG, Yao X: The membrane recruitment and recycling hypothesis of gastric HCl secretion. Trends Cell Biol 6: 45-48 (1996).
7. Forte JG, Black JA, Forte TM, Machen TE, Wolosin JM: Ultrastructural changes related to functional activity in gastric oxyntic cells. Am J Physiol 241: G349-G358 (1981).
8. Forte TM, Machen TE, Forte JG: Ultrastructural changes in oxyntic cells associated with secretory function: a membrane-recycling hypothesis. Gastroenterology 73: 941-955 (1977).
9. Hanzel D, Reggio H, Brescher A, Forte JG, Mangeat P: The secretion-stimulated 80K phosphoprotein of parietal cells is ezrin, and has properties of a membrane cytoskeletal linker in the induced apical microvilli. EMBO J 10: 2363-2373 (1991).
10. Helander HF, Hirschowitz BI: Quantitative ultrastructural studies on gastric parietal cells. Gastroenterology 63: 951-961 (1972).
11. Jehl B, Bauer R, Dörge A, Rick R: The use of propane/isopentane mixtures for rapid freezing of biological specimens. J Microsc 123: 307-309 (1981).
12. Mangeat P, Gusdinar T, Sahuquet A, Hanzel DK, Forte JG, Magous R: Acid secretion and membrane reorganization in single gastric parietal cell in primary culture. Biol Cell 69: 223-232 (1990).
13. Ogata T, Yamasaki Y: Scanning EM of resting gastric parietal cells reveals a network of cytoplasmic tubules and cisternae connected to the intracellular canaliculus. Anat Rec 258: 15-24 (2000).
14. Okamoto CT, Duman JG, Tyagarajan K, McDonald KL, Jeng YY, Mckinney J, Forte TM, Forte JG: Clathrin in gastric acid secretory (parietal) cells: biochemical characterization and subcellular localization. Am J Physiol Cell Physiol 279: C833-C851 (2000).
15. Pettitt JM, Humphris DC, Barrett SP, Toh BH, van Driel IR, Gleeson PA: Fast freeze-fixation/freeze-substitution reveals the secretory membranes of the gastric parietal cell as a network of helically coiled tubule. A new model for parietal cell transformation. J Cell Sci 108: 1127-1141 (1995).
16. Sawaguchi A, McDonald KL, Karvar S, Forte JG: A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells. J Microsc 208: 158-166 (2002).
17. +-ATPase in gastric parietal cells. J Histochem Cytochem 52: 77-86 (2004).
18. Sawaguchi A, Ide S, Suganuma T: Application of 10-μm thin stainless foil to a new assembly of the specimen carrier in high-pressure freezing. J Electron Microsc 54: 143-146 (2005).
19. Schettino T, Köhler M, Frömter E: Membrane potentials of individual cells of isolated gastric glands of rabbit. Pflügers Arch 405: 58-65 (1985).
20. Sugai N, Ito S, Ichikawa A, Ichikawa M: The fine structure of the tubulovesicular system in mouse gastric parietal cell processed by cryofixation method. J Electron Microsc 34: 113-122 (1985).
21. Tamura A, Kikuchi S, Hata M, Katsuno T, Matsui T, Hayashi H, Suzuki Y, Noda T, Tsukita S, Tsukita S: Achlorhydria by ezrin knockdown: defects in the formation/expansion of apical canaliculi in gastric parietal cells. J Cell Biol 169: 21-28 (2005).
22. Terada N, Ohno N, Li Z, Fujii Y, Baba T, Ohno S: Detection of injected fluorescence-conjugated IgG in living mouse organs using "in vivo cryotechnique" with freeze-substitution. Microsc Res Tech 66: 173-178 (2005).
23. Urushidani T, Forte JG: Signal transduction and activation of acid secretion in the parietal cell. J Membr Biol 159: 99-111 (1997).
24. Yao X, Forte JG: Cell Biology of acid secretion by the parietal cell. Annu Rev Physiol 65: 103-131 (2003).
25. Yuchi H, Suganuma T, Sawaguchi A, Ide S, Kawano J, Aoki T, Kitamura K, Eto T: Cryofixation processing is an excellent method to improve the retention of adrenomedullin antigenicity. Histochem Cell Biol 118: 259-265 (2002).