Molecular epidemiology of high-level aminoglycoside-resistant enterococci isolated from patients in a university hospital in southern Italy

Journal of Antimicrobial Chemotherapy

Volumn 56, Issue 5, 2005, Pages 827-835

  Zarrilli, Raffaele ^a   Tripodi, Marie Francoise ^b   Di Popolo, Anna ^a   Fortunato, Rosaria ^b   Bagattini, Maria ^a   Crispino, Margherita ^a   Florio, Anna ^b   Triassi, Maria ^a   Utili, Riccardo ^b  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b SECOND UNIVERSITY OF NAPLES   (Italy)

Author keywords

Antimicrobial resistance genes; Mating; Multiplex PCR; Pulsed field gel electrophoresis

Indexed keywords

AMIKACIN; AMINOGLYCOSIDE ANTIBIOTIC AGENT; GENTAMICIN; RIFAMPICIN; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM ISOLATION; CHROMOSOME PAIRING; CONTROLLED STUDY; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; GENE FREQUENCY; GENE FUNCTION; GENE IDENTIFICATION; GENE ISOLATION; GENE TRANSFER; GENETIC EPIDEMIOLOGY; GENOTYPE; HOSPITAL PATIENT; ITALY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHYLOGENETIC TREE; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS;

ACETYLTRANSFERASES; AMIKACIN; AMINOGLYCOSIDES; ANTI-BACTERIAL AGENTS; CHROMOSOMES, BACTERIAL; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; EPIDEMIOLOGY, MOLECULAR; GENE TRANSFER TECHNIQUES; GENE TRANSFER, HORIZONTAL; GENES, BACTERIAL; GENOTYPE; GENTAMICINS; GRAM-POSITIVE BACTERIAL INFECTIONS; HOSPITALS, UNIVERSITY; HUMANS; ITALY; MICROBIAL SENSITIVITY TESTS; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH;

EID: 27744569858     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dki347     Document Type: Article

References (28)

1. Patterson JE, Zervos MJ. High-level gentamicin resistance in Enterococcus: Microbiology, genetic basis and epidemiology. Rev Infect Dis 1990; 12: 644-52.
2. Vandamme P, Vercauteren E, Lammens C et al. Survey of enterococcal susceptibility patterns in Belgium. J Clin Microbiol 1996; 34: 2572-6.
3. Straut M, de Cespedes G, Delbos F et al. Molecular typing of Enterococcus faecalis strains resistant to high levels of gentamicin and isolated in Romania. J Antimicrob Chemother 1997; 39: 483-91.
4. Silverman J, Thal LA, Perri MB et al. Epidemiologic evaluation of antimicrobial resistance in community-acquired enterococci. J Clin Microbiol 1998; 36: 830-2.
5. van Den Braak N, van Belkum A, Kreft D et al. The prevalence and clonal expansion of high-level gentamicin-resistant enterococci isolated from blood cultures in a Dutch university hospital. J Antimicrob Chemother 1999; 44: 795-8.
6. Udo EE, Al-Sweih N, John P et al. Characterization of high-level aminoglycoside-resistant enterococci in Kuwait hospitals. Microb Drug Resist 2004; 10: 139-45.
7. Fridkin SK, Lawton R, Edwards JR et al. Monitoring antimicrobial use and resistance: Comparison with a national benchmark on reducing vancomycin use and vancomycin-resistant enterococci. Emerg Infect Dis 2002; 7: 702-7.
8. Goossens H, Jabes D, Rossi R et al. European survey of vancomycin-resistant enterococci in at-risk hospital wards and in vitro susceptibility testing of ramoplanin against these isolates. J Antimicrob Chemother 2003; 51 Suppl 3: 5-12.
9. Moellering RC. The Garrod lecture - the enterococcus: A classic example of the impact of antimicrobial resistance on therapeutic options. J Antimicrob Chemother 1991; 28: 1-12.
10. Landman D, Quale JM. Management of infections due to resistant enterococci: A review of therapeutic options. J Antimicrob Chemother 1997; 40: 161-70.
11. Graham JC, Gould FK. Role of aminoglycosides in the treatment of bacterial endocarditis. J Antimicrob Chemother 2002; 49: 437-44.
12. Chow JW. Aminoglycoside resistance in enterococci. Clin Infect Dis 2000; 31: 586-99.
13. del Campo R, Ruiz-Garbajosa P, Sanchez-Moreno MP et al. Antimicrobial resistance in recent fecal enterococci from healthy volunteers and food handlers in Spain: Genes and phenotypes. Microb Drug Resist 2003; 9: 47-60.
14. Donabedian SM, Thal LA, Hershberger E et al. Molecular characterization of gentamicin-resistant Enterococci in the United States: Evidence of spread from animals to humans through food. J Clin Microbiol 2003; 41: 1109-13.
15. Angeletti S, Lorino G, Gherardi G et al. Routine molecular identification of enterococci by gene-specific PCR and 16S ribosomal DNA sequencing. J Clin Microbiol 2001; 39: 794-7.
16. National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk Susceptibility Tests: Approved Standard M2-A7. NCCLS, Wayne, PA, USA, 1999.
17. National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically: Approved Standard M7-A5. NCCLS, Wayne, PA, USA, 2000.
18. Morrison D, Woodford N, Barret SP et al. DNA banding pattern polymorphism in vancomycin-resistant Enterococcus faecium and criteria for defining strains. J Clin Microbiol 1999; 37: 1084-91.
19. Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979; 76: 5269-73.
20. Tenover FC, Arbeit RD, Goering RV et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-9.
21. Vakulenko SB, Donabedian SM, Voskresenkiy AM et al. Multiplex PCR for detection of aminoglycoside resistance genes in enterococci. Antimicrob Agents Chemother 2003; 47: 1423-6.
22. Werner G, Klare I, Witte W. Large conjugative vanA plasmids in vancomycin-resistant Enterococcus faecium. J Clin Microbiol 1999; 37: 2383-4.
23. Franke AE, Clewell DB. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of 'conjugal' transfer in the absence of a conjugative plasmid. J Bacteriol 1981; 145: 494-502.
24. Tripodi MF, Rambaldi A, Utili R et al. Resistance to aminoglycosides and other antibiotics among clinical isolates of Enterococcus spp. New Microbiol 1995; 18: 319-23.
25. Rice LB, Carias LL. Transfer of Tn5385, a composite, multiresistance chromosomal element from Enterococcus faecalis. J Bacteriol 1998; 180: 714-21.
26. Tomita H, Pierson C, Lim SK et al. Possible connection between a widely disseminated conjugative gentamicin resistance (pMG1-like) plasmid and the emergence of vancomycin resistance in Enterococcus faecium. J Clin Microbiol 2002; 40: 3326-33.
27. Takeuchi K, Tomita H, Fujimoto S et al. Drug resistance of Enterococcus faecium clinical isolates and the conjugative transfer of gentamicin and erythromycin resistance traits. FEMS Microbiol Lett 2005; 243: 347-54.
28. Lester CH, Frimodt-Moller N, Hammerum AM. Conjugal transfer of aminoglycoside and macrolide resistance between Enterococcus faecium isolates in the intestine of streptomycin-treated mice. FEMS Microbiol Lett 2004; 235: 385-91.