Essential Cysteine Residues in Bacillus subtilis Spore Photoproduct Lyase Identified by Alanine Scanning Mutagenesis

Current Microbiology

Volumn 51, Issue 5, 2005, Pages 331-335

  Fajardo Cavazos, Patricia ^a   Rebeil, Roberto ^b   Nicholson, Wayne L ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b SANDIA NATIONAL LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; CYSTEINE; IRON; LYASE; SULFUR;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL SPORE; ENZYME ACTIVITY; ENZYME ASSAY; IN VIVO STUDY; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; ULTRAVIOLET RADIATION; ULTRAVIOLET SPECTROSCOPY; WILD TYPE;

ALANINE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACILLUS SUBTILIS; CONSERVED SEQUENCE; CYSTEINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTRUM ANALYSIS;

BACILLUS (BACTERIUM); BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); CLOSTRIDIUM;

EID: 27744558314     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-005-0052-8     Document Type: Article

References (28)

1. Brettin TS, Bruce D, Challacombe JF, Gilna P, Han C, et al. (2004) Complete genome sequence of Bacillus thuringiensis 97-27. Direct submission to NCBI (http://www.ncbi.nlm.nih.gov/)
2. Bruggemann H, Baumer S, Fricke WF, Wiezer A, Liesegang H, et al. (2003) The genome sequence of Clostridium tetani, the causative agent of tetanus disease. Proc Natl Acad Sci USA 100:1316-1321
3. Cheek J, Broderick JB (2002) Direct H atom abstraction from spore photoproduct C-6 initiates DNA repair in the reaction catalyzed by spore photoproduct lyase: evidence for a reversibly generated adenosyl radical intermediate. J Am Chem Soc 124:2860-2861
4. Cutting SM, Vander Horn PB (1990) Genetic analysis. In: Harwood CR, Cutting SM (eds) Molecular biological methods for Bacillus. Sussex, England: John Wiley & Sons, pp 27-74
5. Fajardo-Cavazos P, Salazar C, Nicholson WL (1993) Molecular cloning and characterization of the Bacillus subtilis spore photoproduct lyase (spl) gene, which is involved in repair of UV-induced DNA damage during spore germination. J Bacteriol 175:1735-1744
6. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557-580
7. Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, et al. (2003) Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis. Nature 423:87-91
8. Mehl R, Begley TP (1999) Mechanistic studies on the repair of a novel DNA photolesion: the spore photoproduct. Organic Lett 1:1065-1066
9. Miller J (1972) Experiments in molecular genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press
10. Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P (2000) Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol Biol Rev 64:548-572
11. Nicholson WL, Setlow P (1990) Sporulation, germination, and outgrowth. In: Harwood CR, Cutting SM (eds) Molecular biological methods for Bacillus. Sussex, England: John Wiley & Sons, pp 391-450
12. Nolling J, Breton G, Omelchenko MV, Markarova KS, Zeng Q, et al. (2001) Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J Bacteriol 183:4823-4838
13. Rasko DA, Ravel J, Okstad OA, Helgason E, Cer RZ, et al. (2004) The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1. Nucleic Acids Res 32:977-988
14. Read TD, Salzberg SL, Pop M, Shumway M, Umayam L, et al. (2002) Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis. Science 296:2028-2033
15. Rebeil R, Nicholson WL (2001) The subunit structure and catalytic mechanism of the Bacillus subtilis DNA repair enzyme spore photoproduct lyase. Proc Natl Acad Sci USA 98:9038-9043
16. Rebeil R, Sun Y, Chooback L, Pedraza-Reyes M, Kinsland C, Begley TP, Nicholson WL (1998) Spore photoproduct lyase from Bacillus subtilis spores is a novel iron-sulfur DNA repair enzyme which shares features with proteins such as class III anaerobic ribonucleotide reductases and pyruvate-formate lyases. J Bacteriol 180:4879-4885
17. Rey MW, Ramaiya P, Nelson BA, Brody-Karpin SD, Zaretsky EJ, et al. (2004) Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species. Genome Biol 5:R77
18. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edition. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press
19. Schaeffer P, Millet J, Aubert J-P (1965) Catabolic repression of bacterial sporulation. Proc Natl Acad Sci USA 54:704-711
20. Setlow P (1988) Small, acid-soluble spore proteins of Bacillus species: structure, synthesis, genetics, function, and degradation. Ann Rev Microbiol 42:319-338
21. Setlow P (1995) Mechanisms for the prevention of damage to DNA in spores of Bacillus species. Ann Rev Microbiol 49:29-54
22. Setlow P (2001) Resistance of spores of Bacillus species to ultraviolet light. Environ Mol Mutagen 38:97-104
23. Shimizu T, Ohtani K, Hirakawa H, Ohshima K, Yamashita A, Shiba T, Ogasawara N, Hattori M, Kuhara S, Hayashi H (2002) Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater. Proc Natl Acad Sci USA 99:996-1001
24. Slieman TA, Rebeil R, Nicholson WL (2000) Spore photoproduct (SP) lyase from Bacillus subtilis specifically binds to and cleaves SP (5-thyminyl-5,6- dihydrothymine) but not cyclobutane pyrimidine dimers in UV-irradiated DNA. J Bacteriol 182:6412-6417
25. Sofia HJ, Chen G, Hetzler BG, Reyes-Spindola JF, Miller NE (2001) Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods. Nucleic Acids Res 29:1097-1106
26. Takami H, Nakasone K, Takaki Y, Maeno G, Sasaki Y, et al. (2000) Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis. Nucleic Acids Res 28:4317-4331
27. Takami H, Takaki Y, Uchiyama I (2002) Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments. Nucleic Acids Res 30:3927-3935
28. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119