Immunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of Groundnut bud necrosis virus

Journal of Virological Methods

Volumn 130, Issue 1-2, 2005, Pages 162-164

  Jain, R K ^a   Pandey, Amar N ^a   Krishnareddy, M ^b   Mandal, Bikash ^a  

^a INDIAN AGRICULTURAL RESEARCH INSTITUTE   (India)

^b INDIAN INSTITUTE OF HORTICULTURAL RESEARCH   (India)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; HISTIDINE; HYBRID PROTEIN; NUCLEOCAPSID PROTEIN; POLYCLONAL ANTISERUM; RECOMBINANT PROTEIN; RESIN;

ARTICLE; BACTERIAL CELL; CLONING VECTOR; COWPEA; DIAGNOSTIC PROCEDURE; DILUTION; ESCHERICHIA COLI; GENE EXPRESSION; GROUNDNUT BUD NECROSIS VIRUS; HOST; IN VITRO STUDY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SERODIAGNOSIS; SOLUBILITY; TOSPOVIRUS; VIRAL GENETICS; VIRUS ISOLATION; WATERMELON BUD NECROSIS VIRUS;

AGRICULTURE; ANIMALS; ARACHIS HYPOGAEA; CITRULLUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNE SERA; NUCLEOCAPSID PROTEINS; PLANT DISEASES; RABBITS; TOSPOVIRUS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GROUNDNUT BUD NECROSIS VIRUS; TOSPOVIRUS; WATERMELON BUD NECROSIS VIRUS;

EID: 27744545042     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2005.06.018     Document Type: Article

References (15)

1. Anonymous, 1997. The QIAexpressionist. A handbook for high level expression and purification of 6x His-tagged proteins.
2. A.I. Bhat, R.K. Jain, A. Varma, and S.K. Lal Nucleocapsid protein gene sequence studies suggest that soybean bud blight is caused by a strain of Groundnut bud necrosis virus Curr. Sci. 82 2002 1389 1392
3. M.F. Clark, and M. Bar-Joseph Enzyme immunosorbent assays in plant virology K. Maramorsch H. Koprowski Methods in Virology vol. II 1984 Academic Press New York 51 85
4. M. Hema, N. Kirthi, P. Sreenivasulu, and H.S. Savithri Development of recombinant coat protein antibody based IC-RT-PCR for detection and discrimination of sugarcane streak mosaic virus isolates from Southern India Arch. Virol. 148 2003 1185 1193
5. R.K. Jain, H.R. Pappu, S.S. Pappu, and M. Krishna Reddy Watermelon bud necrosis tospovirus is a distinct virus species belonging to serogroup IV Arch. Virol. 143 1998 1637 1644
6. R.K. Jain, S.M. Paul Khurana, A.I. Bhat, and V. Chaudhary Nucleocapsid protein gene sequence studies confirm that potato stem necrosis is caused by a strain of Groundnut bud necrosis virus Indian Phytopath. 57 2004 169 173
7. 4 Nature 227 1970 680 685
8. B. Mandal, R.K. Jain, V. Choudhary, and A. Varma First report of natural infection of Luffa acutangula by Watermelon bud necrosis virus in India Plant Dis. 87 2003 598
9. J.W. Moyer Tospoviruses (Bunyaviridae) Webster Granoff Encyclopedia of Virology 1999 Academic Press New York 1803 1807
10. I.J. O' Donell, D.D. Shukla, and K.H. Gough Electro-blot immunoassay of virus infected plant sap-A powerful technique for detecting plant viruses J. Virol. Methods 4 1982 19 26
11. M. Reddy, D.V.R. Reddy, and A. Appa Rao A new record of virus disease on peanut Plant Dis. Rept. 52 1968 494 495
12. B.C. Rodoni, J.L. Dale, and R.M. Harding Characterization and expression of the coat protein-coding region of banana bract mosaic potyvirus, development of diagnostic assaya and detection of the virus in banana plants from five countries in southeast Asia Arch. Virol. 144 1999 1725 1737
13. J. Sambrook, and D.W. Russell Molecular Cloning: A laboratory manual 2001 Cold Spring Harbour Laboratory Press New York
14. Ho. Thien Xuan, A.I. Bhat, and R.K. Jain Mungbean necrosis disease caused by a strain of Groundnut bud necrosis tospovirus Indian Phytopath. 56 2003 54 60
15. K. Umamaheswaran, R.K. Jain, A.I. Bhat, and Y.S. Ahlawat Biological and molecular charecterization of a tospovirus isolate from tomato and its relationship with other tospoviruses Indian Phytopath. 56 2003 168 173