Heterologous expression of biologically active porcine lactoferrin in Pichia pastoris yeast

Veterinary Research Communications

Volumn 29, Issue SUPPL. 2, 2005, Pages 379-382

  Pecorini, Chiara ^a   Savazzini, F ^b   Martino, P A ^a   Fusi, E ^a   Fogher, C ^c   Baldi, A ^a  

^a UNIVERSITY OF MILAN   (Italy)

^b RESEARCH AND INNOVATION CENTRE   (Italy)

^c University of Piacenza   (Italy)

Author keywords

Biological activity; Heterologous expression; Lactoferrin; Pichia pastoris; Piglets

Indexed keywords

COMPLEMENTARY DNA; GENOMIC DNA; LACTOFERRIN;

ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CONCENTRATION RESPONSE; CONTROLLED STUDY; HETEROLOGOUS EXPRESSION; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SWINE; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; DNA, COMPLEMENTARY; ESCHERICHIA COLI; FEMALE; LACTOFERRIN; MICROBIAL SENSITIVITY TESTS; PICHIA; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SWINE;

BACTERIA (MICROORGANISMS); PICHIA PASTORIS; SUIDAE; SUS SCROFA;

EID: 27744526854     PISSN: 01657380     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11259-005-0086-1     Document Type: Article

References (11)

1. Baldi, A., Bontempo, V., Dell'Orto, V., Cheli, F., Fantuz, F. and Savoini, G., 1999. Effects of dietary chromium-yeast in weaning-stressed piglets. Canadian Journal of Animal Science, 79, 369-374
2. Blum, J.W. and Baumrucker, C.R., 2002. Colostral and milk insulin-like growth factors and related substances: Mammary gland and neonatal (intestinal and systemic) targets. Domestic Animal Endocrinology, 23, 101-110 10.1016/S0739-7240(02)00149-2
3. Brock, J.H., 2002. The physiology of lactoferrin. Biochemistry and Cell Biology, 80, 1-6 10.1139/o01-212
4. Cereghino, G.P.L. and Cregg, J.M., 1999. Applications of yeast in biotechnology: Protein production and genetic analysis. Current Opinion in Biotechnology, 10, 422-427 10.1016/S0958-1669(99)00004-X
5. Chen, P.W., Ho, S.P., Shyu, C.L. and Mao, F.C., 2004. Effects of bovine lactoferrin hydrolysate on the in vitro antimicrobial susceptibility of Escherichia coli strains isolated from baby pigs. American Journal of Veterinary Research, 65, 131-137 14974567
6. Elliot, J.I., Senft, B., Erhardt, G. and Fraser, D., 1984. Isolation of lactoferrin and its concentration in sow's colostrum and milk during a 21-day lactation. Journal of Animal Science, 59, 1080-1084 6511679
7. Hoek, K.S., Milne, J.M., Grieve, P.A., Dionysius, D.A. and Smith, R., 1997. Antibacterial activity of bovine lactoferrin-derived peptides. Antimicrobial Agents and Chemotherapy, 41, 54-59 8980754
8. Kim, W.S., Ohashi, M., Tanaka, T., Kumura, H., Kim, G.Y., Kwon, I.K., Goh, J.S. and Shimazaki, K., 2004. Growth-promoting effects of lactoferrin on L. acidophilus and Bifidobacterium spp. Biometals, 17, 279-283 10.1023/B:BIOM.0000027705.57430.f1
9. Schanbacher, F.L., Talhouk, R.S. and Murray, F.A., 1997. Biology and origin of bioactive peptides in milk. Livestock Production Science, 50, 105-123 10.1016/S0301-6226(97)00082-1
10. Sherman, M.P., Bennett, S.H., Hwang, F.F.Y. and Yu, C., 2004. Neonatal small bowel epithelia: Enhancing anti-bacterial defense with lactoferrin and Lactobacillus GG. Biometals, 17, 285-289 10.1023/ B:BIOM.0000027706.51112.62
11. Wang, S.H., Yang, T.S., Lin, S.M., Tsai, M.S., Wu, S.C. and Mao, S.J.T., 2002. Expression, characterization and purification of recombinant porcine lactoferrin in Pichia pastoris. Protein Expression and Purification, 25, 41-49 10.1006/prep.2001.1607