High-throughput sequencing of high copy number plasmids from bacterial cultures by heat lysis

BioTechniques

Volumn 39, Issue 3, 2005, Pages 304-308

  Ganguly, Tapan ^a   Chen, Peiqin ^a   Teetsel, Rebecca ^a   Zhang, Lan Ping ^a   Papaioannou, Elias ^a   Cianciarulo, Joseph ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; PLASMID DNA; BACTERIAL DNA;

ARTICLE; BACTERIUM CULTURE; DNA LIBRARY; DNA SEQUENCE; ELECTROPHORESIS; LYSIS; POLYMERASE CHAIN REACTION; SCREENING; CELL FRACTIONATION; CULTURE TECHNIQUE; ESCHERICHIA COLI; GENE DOSAGE; GENETICS; HEAT; METHODOLOGY; PLASMID;

BACTERIA (MICROORGANISMS);

CELL CULTURE TECHNIQUES; CELL FRACTIONATION; DNA, BACTERIAL; ESCHERICHIA COLI; GENE DOSAGE; HEAT; PLASMIDS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 27744504012     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05393BM02     Document Type: Article

References (4)

1. Gladden, J.G., R. Ray, R.D. Gietz, and M.R. Mowat. 2000. Rapid screening of plasmid DNA by direct sequencing from bacterial colonies. BioTechniques 29:436-437.
2. Kilger, C., M. Krings, H. Poinar, and S. Paabo. 1997. "Colony sequencing": direct sequencing of plasmid DNA from bacterial colonies. BioTechniques 22:412-418.
3. Krishnan, B.R., R.W. Blakesley, and D.E. Berg. 1991. Linear amplification DNA sequencing directly from single phage plaques and bacterial colonies. Nucleic Acids Res. 19:1153.
4. Chen, Q., C. Neville, A. MacKenzie, and R.G. Korneluk. 1996. Automated DNA sequencing requiring no DNA template purification. BioTechniques 21:453-457.