mRNA translation is not a prerequisite for small interfering RNA-mediated mRNA cleavage

Differentiation

Volumn 73, Issue 6, 2005, Pages 287-293

  Sen, George L ^a   Wehrman, Tom S ^a   Blau, Helen M ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Gene regulation; RISC; RNAi; siRNA; Translation

Indexed keywords

CLOTRIMAZOLE; CYCLOHEXIMIDE; DEFEROXAMINE MESYLATE; FERRIC AMMONIUM CITRATE; IRON; RNA INDUCED SILENCING COMPLEX; SMALL INTERFERING RNA;

ARTICLE; CONTROLLED STUDY; DEGRADATION; DRUG ACTIVITY; DRUG INHIBITION; GENE CONTROL; GENE SILENCING; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA TRANSLATION;

EID: 27744497487     PISSN: 03014681     EISSN: 14320436     Source Type: Journal    
DOI: 10.1111/j.1432-0436.2005.00029.x     Document Type: Article

References (20)

1. Aktas, H., Fluckiger, R., Acosta, J.A., Savage, J.M., Palakurthi, S.S. and Halperin, J.A. (1998) Depletion of intracellular Ca2+ stores, phosphorylation of eIF2alpha, and sustained inhibition of translation initiation mediate the anticancer effects of clotrimazole. Proc Natl Acad Sci USA 95:8280-8285.
2. Carrasco, L., Fernandez-Puentes, C. and Vazquez, D. (1976) Antibiotics and compounds affecting tanslation by eukaryotic ribosomes. Specific enhancement of aminoacyl-tRNA binding by methylaxnthines. Mol Cell Biochem 10:97-122.
3. Fire, A., Xu., S., Montgomery, M.K., Kostas, S.A., Driver, S.E. and Mello, C.C. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811.
4. Giu, S. and Rossi, J.J. (2005) Uncoupling of RNAi from active translation in mammalian cells. RNA 11:38-44.
5. Goossen, B., Caughman, S.W., Harford, J.B., Klausner, R.D. and Hentze, M.W. (1990) Translational repression by a complex between the iron-responsive element of ferritin mRNA and its specific cytoplasmic binding protein is position-dependent in vivo. EMBO J 9:4127-4133.
6. Goossen, B. and Hentze, M.W. (1992) Position is the critical determinant for function of iron-responsive elements as translational regulators. Mol Cell Biol 12:1959-1966.
7. Hannon, G.J. (2002) RNA interference. Nature 418:244-251.
8. Hentze, M.W., Muckenthaler, M.U. and Andrews, N.C. (2004) Balancing acts: molecular control of mammalian iron metabolism. Cell 117:285-297.
9. Iwai, K., Klausner, R.D. and Rouault, T.A. (1995) Requirements for iron-regulated degradation of the RNA binding protein, iron regulatory protein 2. EMBO J 14:5350-5357.
10. Kennerdell, J.R., Yamaguchi, S. and Carthew, R.W. (2002) RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E. Genes Dev 16:1884-1889.
11. Liu, J., Carmell, M.A., Rivas, F.V., Marsden, C.G. and Hannon, G.J. (2004) Argonaute2 is the catalytic engine of mammalian RNAi. Science 305:1437-1441.
12. Macchi, P., Hemraj, I., Goetze, B., Grunewald, B., Mallardo, M. and Kiebler, M.A. (2003) A GFP-based system to uncouple mRNA transport from translation in a single living neuron. Mol Biol Cell 14:1570-1582.
13. Meister, G. and Tuschl, T. (2004) Mechanisms of gene silencing by double-stranded RNA. Nature 431:343-349.
14. Mourelatos, Z., Dostie, J., Paushkin, S., Sharma, A., Charroux, B., Abel, L., Rappsilber, J., Mann, M. and Dreyfuss, G. (2002) miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. Genes Dev 16:720-728.
15. Muckenthaler, M., Gray, N.K. and Hentze, M.W. (1998) IRP-1 binding to ferritin mRNA prevents the recruitment of the small ribosomal subunit by the cap-binding complex eIF4F. Mol Cell 2:383-388.
16. Pham, J.W., Pellino, J.L., Lee, Y.S., Carthew, R.W. and Sontheimer, E.J. (2004) A dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila. Cell 117:83-94.
17. Shi, Y. (2003) Mammalian RNAi for the masses. Trends Genet 19:9-12.
18. Sen, G., Wehrman, T.S., Myers, J.W. and Blau, H.M. (2004) Restriction enzyme-generated siRNA (REGS) vectors and libraries. Nat Genet 36:183-189.
19. Wang, Q. and Carmichael, G.G. (2004) Effects of length and location on the cellular response to double-stranded RNA. Microbiol Mol Biol Rev 68:432-452 (table of contents).
20. Zamore, P.D., Tuschl, T., Sharp, P.A. and Bartel, D.P. (2000) RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101: 25-33.