Analysis of a heme-dependent signal transduction system in Corynebactenum diphtheriae: Deletion of the chrAS genes results in heme sensitivity and diminished heme-dependent activation of the hmuO promoter

Infection and Immunity

Volumn 73, Issue 11, 2005, Pages 7406-7412

  Bibb, Lori A ^a   King, Natalie D ^a,c   Kunkle, Carey A ^a   Schmitt, Michael P ^a,b  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^c UNIVERSITY AT BUFFALO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; HEME; HEME OXYGENASE; HEMOGLOBIN; IRON; PHOSPHOTRANSFERASE;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIUM CULTURE; CELL VIABILITY; CHRA GENE; CHRS GENE; CLONE; CORYNEBACTERIUM DIPHTHERIAE; DTXR GENE; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION; GENE FUSION; GENE MUTATION; GENE REPRESSION; HMUO GENE; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PRIORITY JOURNAL; PROMOTER REGION; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; TRANSCRIPTION INITIATION;

BACTERIAL PROTEINS; CORYNEBACTERIUM DIPHTHERIAE; DNA, INTERGENIC; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; HEME; HEME OXYGENASE (DECYCLIZING); MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); SIGNAL TRANSDUCTION; TRANSCRIPTION, GENETIC;

EID: 27744497470     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.73.11.7406-7412.2005     Document Type: Article

References (36)

1. Altschul, S. F., G. Warren, W. Miller, E. W. Meyers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Barksdale, W. L., and A. M. Pappenheimer, Jr. 1954. Phage-host relationships in nontoxigenic and toxigenic diphtheria bacilli. J. Bacteriol. 67:220-232.
3. Boyd, J. M., O. N. Manish, and J. R. Murphy. 1990. Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proc. Natl. Acad. Sci. USA 87: 5968-5972.
4. Cerdeno-Tarraga, A. M., A. Efstratiou, L. G. Dover, M. T. Holden, M. Pallen, S. D. Bentley, G. S. Besra, C. Churcher, K. D. James, A. De Zoysa, T. Chillingworth, A. Cronin, L. Dowd, T. Feltwell, N. Hamlin, S. Holroyd, K. Jagels, S. Moule, M. A. Quail, E. Rabbinowitsch, K. M. Rutherford, N. R. Thomson, L. Unwin, S. Whitehead, B. G. Barrell, and J. Parkhill. 2003. The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129. Nucleic Acids Res. 31:6516-6523.
5. Drazek, E. S., C. A. Hammack, and M. P. Schmitt. 2000. Corynebacterium diphtheriae genes required for acquisition of iron from hemin and hemoglobin are homologous to ABC hemin transporters. Mol. Microbiol. 36:68-84.
6. Everse, J., and H. Nelson. 1997. The toxicities of native and modified hemoglobins. Free Radical Biol. Med. 22:1075-1099.
7. Feese, M. D., E. Pohl, R. K. Holmes, and W. G. J. Hol. 2001. Iron-dependent regulators, p. 850-863. In A. Messerschmidt, R. Huber, T. Poulos, and K. Wieghardt (ed.), Handbook of metalloproteins. John Wiley & Sons, Ltd., Chichester, United Kingdom.
8. Hantke, K. 2005. Bacterial zinc uptake and regulators. Curr. Opin. Microbiol. 8:196-202.
9. Kirby, A. E., D. J. Metzger, E. R. Murphy, and T. D. Connell. 2001. Heme utilization in Bordetella avium is regulated by RhuI, a heme responsive extracytoplasmic function sigma factor. Infect. Immun. 69:6951-6961.
10. Kunkle, C. A., and M. P. Schmitt. 2003. Analysis of the Corynebacterium diphtheriae DtxR regulon: identification of a putative siderophore synthesis and transport system that is similar to the Yersinia high-pathogenicity island-encoded yersiniabactin synthesis and uptake system. J. Bacteriol. 185:6826-6840.
11. Kunkle, C. A., and M. P. Schmitt. 2005. Analysis of a DtxR-regulated iron transport and siderophore biosynthesis gene cluster in Corynebacterium diphtheriae. J. Bacteriol. 187:422-433.
12. Lopez-Maury, L., M. Dominguez, F. J. Florencio, and J. C. Reyes. 2002. A two-component signal transduction system involved in nickel sensing in the cyanobacterium Synechocystis sp. PCC 6803. Mol. Microbiol. 43:247-256.
13. Maines, M. D. (ed.). 1992. Heme oxygenase: clinical applications and functions, p. 109-144. CRC Press, Boca Raton, Fla.
14. Mietzner, T. A., and S. A. Morse. 1994. The role of iron-binding proteins in the survival of pathogenic bacteria. Annu. Rev. Nutr. 14:471-493.
15. Munson, G. P., D. L. Lam, F. W. Outten, and T. V. O'Halloran. 2000. Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12. J. Bacteriol. 182:5864-5871.
16. Ochsner, U. A., and M. L. Vasil. 1996. Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron regulated genes. Proc. Natl. Acad. Sci. USA 93:4409-4414.
17. Otto, B. R., V.-V. Vught, A. M. Verweij-van Vught, and D. M. MacLaren. 1992. Transferrins and heme compounds as iron sources for pathogenic bacteria. Crit. Rev. Microbiol. 18:217-233.
18. Pallen, M. J., A. C. Lam, M. Antonio, and K. Dunbar. 2001. An embarrassment of sortases-a richness of substrates? Trends Microbiol. 9:97-102.
19. Parkinson, J. S., and E. C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26:71-112.
20. Qian, Y., J. H. Lee, and R. K. Holmes. 2002. Identification of a DtxR-regulated operon that is essential for siderophore-dependent iron uptake in Corynebacterium diphtheriae. J. Bacteriol. 184:4846-4856.
21. Ratliff, M., W. Zhu, R. Deshmukh, A. Wilks, and I. Stojilkovic. 2001. Homologues of neisserial heme oxygenases in gram-negative bacteria: degradation of heme by the product of the pigA gene of Pseudomonas aeruginosa. J. Bacteriol. 183:6394-6403.
22. Rossi, M.-S., A. Paquelin, J. M. Ghigo, and C. Wandersman. 2003. Haemophore-mediated signal transduction across the bacterial cell envelope in Serratia marcescens: the inducer and the transported substrate are different molecules. Mol. Microbiol. 48:1467-1480.
23. Schmitt, M. P. 1997. Utilization of host iron sources by Corynebacterium diphtheriae: identification of a gene whose product is homologous to eukaryotic heme oxygenaese and is required for acquisition of iron from heme and hemoglobin. J. Bacteriol. 179:838-845.
24. Schmitt, M. P. 1997. Transcription of the Corynebacterium diphtheriae hmuO gene is regulated by iron and heme. Infect. Immun. 65:4634-4641.
25. Schmitt, M. P. 1999. Identification of a two-component signal transduction system from Corynebacterium diphtheriae that activates gene expression in response to the presence of heme and hemoglobin. J. Bacteriol. 181:5330-5340.
26. Schmitt, M. P. 2004. Corynebacterium diphtheriae p. 344-359. In J. H. Crosa, A. R. Mey, and S. M. Payne (ed.), Iron transport in bacteria. ASM Press, Washington, D.C.
27. Schmitt, M. P., and E. S. Drazek. 2001. Construction and consequences of directed mutations affecting the hemin receptor in pathogenic Corynebacterium species. J. Bacteriol. 183:1476-1481.
28. Schmitt, M. P., and R. K. Holmes. 1991. Iron-dependent regulation of diphtheria toxin and siderophore expression by the cloned Corynebacterium diphtheriae repressor gene dtxR in C. diphtheriae C7 strains. Infect. Immun. 59:1899-1904.
29. Schmitt, M. P., and R. K. Holmes. 1991. Characterization of a defective diphtheria toxin repressor (dtxR) allele and analysis of dtxR transcription in wildtype and mutant strains of Corynebacterium diphtheriae. Infect. Immun. 59:3903-3908.
30. Stojiljkovic, I., and K. Hantke. 1994. Transport of haemin across the cytoplasmic membrane through a haemin-specific periplasmic binding-protein- dependent transport system in Yersinia enterocolitica. Mol. Microbiol. 13:719-732.
31. Stojiljkovic, I., and D. Perkins-Balding. 2002. Processing of heme and heme-containing proteins by bacteria. DNA Cell Biol. 21:281-295.
32. Vanderpool, C. K., and S. K. Armstrong. 2003. Heme-responsive transcriptional activation of Bordetella bhu genes. J. Bacteriol. 185:909-917.
33. Wilks, A. 2001. The ShuS protein of Shigella dysenteriae is a heme-sequestering protein that also binds DNA. Arch. Biochem. Biophys. 387:137-142.
34. Wilks, A. 2002. Heme oxygenase: evolution, structure, and mechanism. Antioxid. Redox Signal. 4:603-614.
35. Wilks, A., and M. P. Schmitt. 1998. Expression and characterization of a heme oxygenase (HmuO) from Corynebacterium diphtheriae. J. Biol. Chem. 273:837-841.
36. Zhu, W., D. J. Hunt, A. R. Richardson, and I. Stojilkovic. 2000. Use of heme compounds as iron sources by pathogenic Neisseria requires the product of the hemO gene. J. Bacteriol. 182:439-447.