Visualization of binding and transcytosis of botulinum toxin by human intestinal epithelial cells

Journal of Pharmacology and Experimental Therapeutics

Volumn 315, Issue 3, 2005, Pages 1028-1035

  Ahsan, Chowdhury R ^a   Hajnóczky, György ^a   Maksymowych, Andrew B ^a   Simpson, Lance L ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOTULINUM TOXIN A; NEUROTOXIN;

ANIMAL TISSUE; APICAL MEMBRANE; ARTICLE; BASOLATERAL MEMBRANE; BINDING SITE; CELL NUCLEUS; CELL SURFACE; CONTROLLED STUDY; ENDOCYTOSIS; FEMALE; HUMAN; HUMAN CELL; INTERNALIZATION; INTESTINE EPITHELIUM CELL; MOUSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RECEPTOR BINDING; TRANSCYTOSIS; TRANSPORT VESICLE;

ANIMALS; BIOLOGICAL TRANSPORT; BOTULINUM TOXIN TYPE A; CELL CULTURE TECHNIQUES; CELL LINE, TRANSFORMED; CELL POLARITY; EPITHELIAL CELLS; HUMANS; INTESTINES; KINETICS; MICE; MICE, INBRED STRAINS; MODELS, MOLECULAR; PROTEIN BINDING; TOXICITY TESTS;

EID: 27744456489     PISSN: 00223565     EISSN: 15210103     Source Type: Journal    
DOI: 10.1124/jpet.105.092213     Document Type: Article

References (21)

1. 125I-labeled botulinum neurotoxins with nerve terminals: I. Ultrastructural autoradiographic localization and quantitation of distinct membrane acceptors for types A and B on motor nerves. J Cell Biol 103:521-534.
2. 125I-labeled botulinum neurotoxins with nerve terminals: II. Autoradiographic evidence for its uptake into motor nerves by acceptor-mediated endocytosis. J Cell Biol 103:535-544.
3. Bonventre PF (1979) Absorption of botulinal toxin from the gastrointestinal tract. Rev Infect Dis 1:663-667.
4. DasGupta BR and Sathyamoorthy V (1984) Purification and amino acid composition of type A botulinum neurotoxin. Toxicon 22:415-424.
5. Habermann E and Dreyer F (1986) Clostridial neurotoxins: handling and action at the cellular and molecular level. Curr Top Micro Immun 129:94-179.
6. Halpern JL and Neale EA (1995) Neurospecific binding, internalization and retrograde axonal transport. Curr Top Microbiol Immun 195:221-241.
7. Herreros J, Lalli G, Montecucco C, and Schiavo G (1999) Pathophysiological properties of clostridial neurotoxins, in The Comprehensive Sourcebook of Bacterial Protein Toxins (Alouf J and Freer J eds) pp 202-228, Academic Press, New York.
8. Humeau Y, Doussau F, Grant NJ, and Poulain B (2000) How botulinum and tetanus neurotoxins block neurotransmitter release. Biochimie 82:427-446.
9. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227:680-685.
10. Lalli G, Herreros J, Osborne SL, Montecucco C, Rossetto O, and Schiavo G (1999) Functional characterization of tetanus and botulinum neurotoxin binding domains. J Cell Sci 112:2715-2724.
11. Madara JL, Stafford J, Dharmsathaphorn K, and Carlson S (1987) Structural analysis of a human intestinal epithelial cell line. Gastroenterology 92:1133-1145.
12. Maksymowych AB, Reinhard M, Malizio CJ, Goodnough MC, Johnson EA, and Simpson LL (1999) Pure botulinum neurotoxin is absorbed from the stomach and small intestine and produces peripheral neuromuscular blockade. Infect Immun 67:4708-4712.
13. Maksymowych AB and Simpson LL (1998) Binding and transcytosis of botulinum neurotoxin by polarized human colon carcinoma cells. J Biol Chem 273:21950-21957.
14. Maksymowych AB and Simpson LL (2004) Structural features of the binding neurotoxin molecule that govern binding and transcytosis across polarized human intestinal epithelial cells. J Pharmacol Exp Ther 310:1-9.
15. Sakaguchi G (1983) Clostridium botulinum toxins. Pharmacol Ther 19:165-194.
16. Schiavo G, Matteoli M, and Montecucco C (2000) Neurotoxins affecting neuroexocytosis. Physiol Rev 80:717-766.
17. Simpson LL (2004) Identification of the major steps in botulinum toxin action. Annu Rev Pharmacol Toxicol 44:167-193.
18. Simpson LL, Maksymowych AB, and Hao S (2001) The role of zinc binding in the biological activity of botulinum toxin. J Biol Chem 276:27034-27041.
19. Simpson LL, Maksymowych AB, and Kiyatkin N (1999) Botulinum toxin as a carrier for oral vaccines. Cell Mol Life Sci 56:47-61.
20. Simpson LL, Schmidt JJ, and Middlebrook JL (1988) Methods Enzymology (Harshman S ed) pp 76-85, Academic Press, New York.
21. Wellhöner HH (1992) Tetanus and botulinum neurotoxins, in Handbook of Experimental Pharmacology, Volume 102, Selective Neurotoxicity (Herken H and Hucho F eds) pp 357-417, Springer-Verlag, Berlin, Germany.