Trypsin is associated with the rotavirus capsid and is activated by solubilization of outer capsid proteins

Journal of General Virology

Volumn 86, Issue 11, 2005, Pages 3143-3151

  Benureau, Yann ^a   Huet, Jean Claude ^b   Charpilienne, Annie ^a   Poncet, Didier ^a   Cohen, Jean ^a  

^a CNRS   (France)

^b CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

APROTININ; CALCIUM CHELATING AGENT; CAPSID PROTEIN; MESSENGER RNA; PROTEINASE INHIBITOR; TRYPSIN; TRYPSIN INHIBITOR; VIRUS PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; IN VITRO STUDY; IN VIVO STUDY; MASS SPECTROMETRY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SYNTHESIS INHIBITION; PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; RNA SYNTHESIS; ROTAVIRUS; SOLUBILIZATION; VIRUS CAPSID; VIRUS CELL INTERACTION; VIRUS INFECTIVITY; VIRUS ISOLATION; VIRUS PARTICLE; VIRUS REPLICATION;

ANTIGENS, VIRAL; CAPSID PROTEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; MODELS, BIOLOGICAL; ROTAVIRUS; TRYPSIN; VIRION;

ANIMALIA; MIRIDAE; ROTAVIRUS;

EID: 27644563706     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/vir.0.81045-0     Document Type: Article

References (29)

1. Charpilienne, A., Abad, M. J., Michelangeli, F., Alvarado, F., Vasseur, M., Cohen, J. & Ruiz, M. C. (1997). Solubilized and cleaved VP7, the outer glycoprotein of rotavirus, induces permeabilization of cell membrane vesicles. J Gen Virol 78, 1367-1371.
2. 2+ gradient during entry of rotavirus into MA104 cells. J Virol 76, 13083-13087.
3. Ciarlet, M., Hidalgo, M., Gorziglia, M. & Liprandi, F. (1994). Characterization of neutralization epitopes on the VP7 surface protein of serotype G11 porcine rotaviruses. J Gen Virol 75, 1867-1873.
4. Clark, S. M., Roth, J. R., Clark, M. L., Barnett, B. B. & Spendlove, R. S. (1981). Trypsin enhancement of rotavirus infectivity: mechanism of enhancement. J Virol 39, 816-822.
5. Cohen, J., Laporte, J., Charpilienne, A. & Scherrer, R. (1979). Activation of rotavirus RNA polymerase by calcium chelation. Arch Virol 60, 177-186.
6. Crawford, S. E., Mukherjee, S. K., Estes, M. K., Lawton, J. A., Shaw, A. L., Ramig, R. F. & Prasad, B. V. (2001). Trypsin cleavage stabilizes the rotavirus VP4 spike. J Virol 75, 6052-6061.
7. Denisova, E., Dowling, W., LaMonica, R., Shaw, R., Scarlata, S., Ruggeri, F. & Mackow, E. R. (1999). Rotavirus capsid protein VP5* permeabilizes membranes. J Virol 73, 3147-3153.
8. Dormitzer, P. R., Nason, E. B., Prasad, B. V. & Harrison, S. C. (2004). Structural rearrangements in the membrane penetration protein of a non-enveloped virus. Nature 430, 1053-1058.
9. Estes, M. K., Graham, D. Y., Smith, E. M. & Gerba, C. P. (1979). Rotavirus stability and inactivation. J Gen Virol 43, 403-409.
10. Kaljot, K. T., Shaw, R. D., Rubin, D. H. & Greenberg, H. B. (1988). Infectious rotavirus enters cells by direct cell membrane penetration, not by endocytosis. J Virol 62, 1136-1144.
11. Katyal, R., Rana, S. V., Ojha, S., Vaiphei, K., Singh, V. & Singh, K. (2001). Soybean trypsin inhibitor confers protection against rotavirus infection in infant mice. Trop Gastroenterol 22, 207-210.
12. Klenk, H. D., Rott, R., Orlich, M. & Blodorn, J. (1975). Activation of influenza A viruses by trypsin treatment. Virology 68, 426-439.
13. Liprandi, F., Moros, Z., Gerder, M., Ludert, J. E., Pujol, F. H., Ruiz, M. C., Michelangeli, F., Charpilienne, A. & Cohen, J. (1997). Productive penetration of rotavirus in cultured cells induces coentry of the translation inhibitor alpha-sarcin. Virology 237, 430-438.
14. Lopez, S. & Arias, C. F. (2004). Multistep entry of rotavirus into cells: a Versaillesque dance. Trends Microbiol 12, 271-278.
15. Ludert, J. E., Krishnaney, A. A., Burns, J. W., Vo, P. T. & Greenberg, H. B. (1996). Cleavage of rotavirus VP4 in vivo. J Gen Virol 77, 391-395.
16. Ludert, J. E., Ruiz, M. C., Hidalgo, C. & Liprandi, F. (2002). Antibodies to rotavirus outer capsid glycoprotein VP7 neutralize infectivity by inhibiting virion decapsidation. J Virol 76, 6643-6651.
17. Mendez, I. I., Hermann, L. L., Hazelton, P. R. & Coombs, K. M. (2000). A comparative analysis of freon substitutes in the purification of reovirus and calicivirus. J Virol Methods 90, 59-67.
18. Obert, G., Peiffer, I. & Servin, A. L. (2000). Rotavirus-induced structural and functional alterations in tight junctions of polarized intestinal Caco-2 cell monolayers. J Virol 74, 4645-4651.
19. Overbergh, L., Valckx, D., Waer, M. & Mathieu, C. (1999). Quantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR. Cytokine 11, 305-312.
20. Parashar, U. D., Hummelman, E. G., Bresee, J. S., Miller, M. A. & Glass, R. I. (2003). Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis 9, 565-572.
21. Poncet, D. & Cohen, J. (1989). A plaque hybridization assay for rotaviruses. J Virol Methods 26, 27-33.
22. Prasad, B. V., Crawford, S., Lawton, J. A., Pesavento, J., Hardy, M. & Estes, M. K. (2001). Structural studies on gastroenteritis viruses. Novartis Found Symp 238, 26-37.
23. Ruiz, M. C., Alonso-Torre, S. R., Charpilienne, A., Vasseur, M., Michelangeli, F., Cohen, J. & Alvarado, F. (1994). Rotavirus interaction with isolated membrane vesicles. J Virol 68, 4009-4016.
24. Ruiz, M. C., Abad, M. J., Charpilienne, A., Cohen, J. & Michelangeli, F. (1997). Cell lines susceptible to infection are permeabilized by cleaved and solubilized outer layer proteins of rotavirus. J Gen Virol 78, 2883-2893.
25. 2+ in the replication and pathogenesis of rotavirus and other viral infections. Cell Calcium 28, 137-149.
26. Schwartz-Cornil, I., Benureau, Y., Greenberg, H., Hendrickson, B. A. & Cohen, J. (2002). Heterologous protection induced by the inner capsid proteins of rotavirus requires transcytosis of mucosal immunoglobulins. J Virol 76, 8110-8117.
27. Shah, R. B., Palamakula, A. & Khan, M. A. (2004). Cytotoxicity evaluation of enzyme inhibitors and absorption enhancers in Caco-2 cells for oral delivery of salmon calcitonin. J Pharm Sci 93, 1070-1082.
28. Vonderfecht, S. L., Miskuff, R. L., Wee, S. B., Sato, S., Tidwell, R. R., Geratz, J. D. & Yolken, R. H. (1988). Protease inhibitors suppress the in vitro and in vivo replication of rotavirus. J Clin Invest 82, 2011-2016.
29. Ye, S. & Goldsmith, E. J. (2001). Serpins and other covalent protease inhibitors. Curr Opin Struct Biol 11, 740-745.