SYBR Green I-induced fluorescence in cultured immune cells: A comparison with Acridine Orange

Acta Histochemica

Volumn 107, Issue 4, 2005, Pages 301-312

  Briggs, Candida ^a   Jones, Mark ^a  

^a UNIVERSITY OF WESTERN SYDNEY   (Australia)

Author keywords

Acridine Orange; Fluorescence microscopy; L23 immune cells; Living cells; SYBR Green I; Uptake

Indexed keywords

BODY FLUIDS; DNA; FLUORESCENCE; IMMUNOLOGY; MICROSCOPIC EXAMINATION; RNA;

ACRIDINE ORANGE; FLUORESCENCE MICROSCOPY; L23 IMMUNE CELLS; SYBR GREEN I; UPTAKE;

CELLS;

ACRIDINE ORANGE; CELL NUCLEUS DNA; DOUBLE STRANDED DNA; DOUBLE STRANDED RNA; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; MITOCHONDRIAL DNA; NUCLEIC ACID; SYBR GREEN I; TRYPAN BLUE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL NUCLEUS; COMPARATIVE STUDY; CONTROLLED STUDY; DNA BINDING; EUCHROMATIN; FLUORESCENCE MICROSCOPY; HETEROCHROMATIN; IMMUNOCOMPETENT CELL; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE; NONHUMAN; RNA BINDING; SENSITIVITY ANALYSIS; STATISTICAL ANALYSIS; TEMPERATURE DEPENDENCE; TRANSPORT KINETICS;

ANIMALIA;

EID: 27644562012     PISSN: 00651281     EISSN: 16180372     Source Type: Journal    
DOI: 10.1016/j.acthis.2005.06.010     Document Type: Article

References (18)

1. M. Capelson, and V.G. Corces Boundary elements and nuclear organization Biol Cell 94 2004 617 629
2. T. Cremer, K. Kupper, S. Dietzel, and S. Fakan Higher order chromatin architecture in the cell nucleus: on the way from structure to function Biol Cell 96 2004 555 567
3. N. Dillon Heterochromatin structure and function Biol Cell 96 2004 631 637
4. J. Dobrucki, and Z. Darzynkiewicz Chromatin condensation and sensitivity of DNA in situ to denaturation during cell cycle and apoptosis - a confocal microscope study Micron 32 2001 645 652
5. R.P. Haugland Handbook of fluorescent probes and research chemicals 9th ed 2002 Molecular Probes Inc. Eugene, OR, USA p. 274, 279, 280, 311-313
6. S. Konnai, T. Usui, K. Ohashi, and M. Onuma The rapid quantitative analysis of bovine cytokine genes by real-time RT-PCR Vet Micro 92 2003 283 294
7. H. Lodish, A. Berk, S.L. Zipursky, P. Matsudaira, D. Baltimore, and J. Darnell Molecular cell biology 4th ed 2000 W.H Freeman and Company New York, USA p. 127, 329
8. M. Lundgren, M. Chow C-, P. Sabbattini, A. Georgiou, S. Minaee, and N. Dillon Transcription factor dosage affects changes in higher order chromatin structure associated with activation of a heterochromatic gene Cell 103 2000 733 743
9. M.B. Lyles, and I.L. Cameron Interactions of the DNA intercalator Acridine Orange, with itself, with caffeine and with double stranded DNA Biophys Chem 96 2002 53 76
10. E. Milot, J. Strouboulis, T. Trimborn, M. Wijgerde, E. De Boer, and A. Langeveld Heterochromatin effects on the frequency and duration of LCR-mediated gene transcription Cell 87 1996 105 114
11. M.A. Myers Direct measurement of cell numbers in microtitre plate cultures using the fluorescent dye SYBR green I J Immuno Methods 212 1998 99 103
12. H.J. Ricketts, A.J. Morgan, D.J. Spurgeon, and P. Kille Measurement of annetocin gene expression: a new reproductive biomarker in earthworm ecotoxicology Ecotoxicol Environ Saf 57 2004 4 10
13. J.M. Rinta-Kanto, M.J. Lehtola, T. Vartiainen, and P.J. Martikainen Rapid enumeration of virus-like particles in drinking water samples using SYBR green I-staining Water Res 38 2004 2614 2618
14. J. Skeidsvoll, and P.M. Ueland Analysis of double-stranded DNA by capillary electrophoresis with laser-induced fluorescence detection using the monomeric dye SYBR Green 1 Anal Biochem 231 1995 359 365
15. T. Suzuki, K. Fujikura, T. Higashiyama, and K. Takata DNA staining for fluorescence and laser confocal microscopy J Histochem Cytochem 45 1997 49 53
16. F. Vitzthum, G. Geiger, H. Bisswanger, H. Brunner, and J. Bernhagen A quantitative fluorescence-based microplate assay for the determination of double-stranded DNA using SYBR Green I and a standard ultraviolet transilluminator gel imaging system Anal Biochem 276 1999 59 64
17. J.L. Zabzdyr, and S.J. Lillard UV- and visible-excited fluorescence of nuclei acids separated by capillary electrophoresis J Chromatogr A 911 2001 269 276
18. H. Zipper, H. Brunner, J. Bernhagen, and F. Vitzthum Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications Nucl Acids Res 32 2004 e103