Expression and characterization of recombinant β-secretase from Trichoplusia ni BTI Tn5B1-4 cells transformed with cDNAs encoding human β1,4-galactosyltransferase and Gal β1,4-GlcNAc α 2,6-sialytransferase

Protein Expression and Purification

Volumn 44, Issue 2, 2005, Pages 87-93

  Kyung, Hwa Chang ^a   Nam, In Baek ^a   Jai, Myung Yang ^b   Jong, Min Lee ^a   Jeon, Hwang Bo ^a   Chung, In Sik ^a  

^a Kyung Hee University   (South Korea)

^b Sogang University   (South Korea)

Author keywords

Secretase; Activity; Gal 1,4 GlcNAc 2,6 sialytransferase; Human 1,4 galactosyltransferase; Purification; Trichoplusia ni BTI Tn5B1 4 cells

Indexed keywords

AMYLOID BETA PROTEIN PRECURSOR INHIBITOR; AMYLOID BETA-PROTEIN PRECURSOR INHIBITOR; ASPARTIC PROTEINASE; BACE1 PROTEIN, HUMAN; BETA D GALACTOSIDE ALPHA 2 6 SIALYLTRANSFERASE; BETA-D-GALACTOSIDE ALPHA 2-6-SIALYLTRANSFERASE; ENZYME INHIBITOR; FLUORESCENT DYE; N ACETYLLACTOSAMINE SYNTHASE; PLANT LECTIN; PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; RICINUS COMMUNIS AGGLUTININ 1; RICINUS COMMUNIS AGGLUTININ-1; SAMBUCUS NIGRA LECTINS; SECRETASE; SIALYLTRANSFERASE;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL LINE; CHEMISTRY; ENZYMOLOGY; GENETIC TRANSFECTION; GENETICS; GLYCOSYLATION; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR WEIGHT; MOTH; TWO DIMENSIONAL GEL ELECTROPHORESIS;

AMYLOID PRECURSOR PROTEIN SECRETASES; ANIMALS; ASPARTIC ENDOPEPTIDASES; CELL LINE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENDOPEPTIDASES; ENZYME INHIBITORS; FLUORESCENT DYES; GLYCOSYLATION; HUMANS; MOLECULAR WEIGHT; MOTHS; N-ACETYLLACTOSAMINE SYNTHASE; PLANT LECTINS; PROTEINS; RECOMBINANT PROTEINS; SIALYLTRANSFERASES; TRANSFECTION;

TRICHOPLUSIA NI;

EID: 27644516583     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.08.010     Document Type: Article

References (24)

1. G.G. Glenner, and C.W. Wong Alzheimer's disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein Biochem. Biophys. Res. Commun. 120 1984 885 890
2. D.J. Selkoe Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease Annu. Rev. Cell Biol. 10 1994 373 403
3. C. Haass, A.Y. Hung, D.J. Selkoe, and D.B. Teplow Mutations associated with a locus for familial Alzheimer's disease result in alternative processing of amyloid beta-protein precursor J. Biol. Chem. 269 1994 17741 17748
4. J. Kang, H.G. Lemaire, A. Unterbeck, J.M. Salsaum, C.L. Masters, K.H. Grzeschik, G. Multhaup, K. Beyreuther, and B. Muller-hill The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor Nature 325 1987 733 736
5. M. Haniu, P. Denis, Y. Young, E.A. Mendiaz, J. Fuller, J.O. Hui, B.D. Bennett, S. Khan, S. Ross, T. Burgess, V. Katta, R. Gorges, R. Vassar, and M. Citron Characterization of Alzheimer's β-secretase protein BACE J. Biol. Chem. 275 2000 21099 21110
6. A. Varki Biological roles of oligosaccharides: all of the theories are correct Glycobiology 3 1994 7 130
7. J. Charlwood, C. Dingwall, R. Matico, L. Hussain, K. Johanson, S. Moore, D.J. Powell, J.M. Skehel, S. Ratcliffe, B. Clarke, J. Trill, S. Sweitzer, and P. Camilleri Characterization of the glycosylation profiles of Alzheimer's beta-secretase protein Asp-2 expressed in a variety of cell lines J. Biol. Chem. 276 2001 16739 16748
8. W. Bruinzeel, J. Yon, S. Giovannelli, and S. Masure Recombinant insect cell expression and purification of human β-secretase (BACE-1) for X-ray crystallography Protein Expr. Purif. 26 2002 139 148
9. W.D. Mallender, D. Yager, L. Onstead, M.R. Nichol, C. Eckman, K. Sambamurti, L.M. Kopcho, J. Marcinkeviciene, R.A. Copeland, and T.L. Rosenberry Characterization of recombinant, soluble beta-secretase from an insect cell expression system Mol. Pharmacol. 59 2001 619 626
10. E. Ailor, N. Takahashi, Y. Tsukamoto, K. Masuda, A.A. Rahman, D.L. Jarvis, Y.C. Lee, and M.J. Betenbaugh N-glycan patterns of human transferrin produced in Trichoplusia ni insect cells: effects of mammalian galactosyltransferase Glycobiology 10 2000 837 847
11. J. Hollister, and D.L. Jarvis Engineering lepidopteran insect cells for sialoglycoprotein production by genetic transformation with mammalian β1,4-galactosyltransferase and α 2,6-sialyltransferase genes Glycobiology 11 2001 1 9
12. K. Breitbach, and D.L. Jarvis Improved glycosylation of a foreign protein by Tn-5B1-4 cells engineered to express mammalian glycosyltransferases Biotechnol. Bioeng. 74 2001 230 239
13. N.-S. Seo, J.R. Hollister, and D.L. Jarvis Mammalian glycosyltransferase expression allows sialoprotein production by baculovirus-infected insect cells Protein Expr. Purif. 22 2001 234 241
14. H.G. Kim, S.M. Yang, Y.C. Lee, S.I. Do, I.S. Chung, and J.M. Yang High-level expression of human glycosyltransferases in insect cells as biochemically active form Biochem. Biophys. Res. Commun. 305 2003 488 493
15. K.H. Chang, J.M. Lee, H.K. Jeon, and I.S. Chung Improved production of recombinant tumstatin in stably transformed Trichoplusia ni BTI Tn 5B1-4 cells Protein Expr. Purif. 35 2004 69 75
16. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 1998 Cold Spring Harbour Laboratory Press New York
17. U.K. Laemmli Cleavage of structural proteins during the assembly of head of bacteriophage Nature 227 1970 680 685
18. D.L. Jarvis, and E.E. Finn Modifying the insect cell N-glycosylation pathway with immediate early baculovirus expression vectors Nat. Biotechnol. 14 1996 1288 1292
19. 2 reduction activity of CO dehydrogenase from Rhodospirillum rubrum Biochemistry 40 2001 7604 7611
20. E. Lullau, A. Kanttinen, J. Hassel, M. Berg, A. Haag-Alvarsson, K. Cederbrant, B. Greenberg, C. Fenge, and F. Schweikart Comparison of batch and perfusion culture in combination with pilot-scale expanded bed purification for the production of soluble recombinant β-secretase Biotechnol. Prog. 19 2003 37 44
21. T.A. Hsu, N. Takahashi, Y. Tsukamoto, K. Kato, I. Shimada, K. Masuda, E. Whiteley, J.-Q. Fan, Y.C. Lee, and M.J. Betenbaugh Differential N-glycan patterns of secreted and intracellular IgG produced in Trichoplusia ni cells J. Biol. Chem. 272 1997 9062 9070
22. B. Abdul-Rahman, E. Ailor, D.L. Jarvis, M.J. Betenbaugh, and Y.C. Lee Beta(1-4)-galactosyltransferase activity in native and engineered insect cells measured with time-resolved europium fluorescence Carbohydr. Res. 337 2002 2181 2186
23. K. Shimomura, and R.D. Bremel Characterization of bovine placental lactogen as a glycoprotein with N-linked and O-linked carbohydrate side chain Mol. Endocrinol. 2 1988 845 853
24. S.J. Pirruccello, and T.W. Lebien Monoclonal antibody BA-1 recognizes a novel human leukocyte cell surface sialoglycoprotein complex J. Immunol. 134 1985 3962 3968