Crystal structure of the E230Q mutant of cAMP-dependent protein kinase reveals an unexpected apoenzyme conformation and an extended N-terminal A helix

Protein Science

Volumn 14, Issue 11, 2005, Pages 2871-2879

  Wu, Jian ^a   Yang, Jie ^a   Kannan, Natarajan ^a   Madhusudan, ^a   Xuong, Nguyen Huu ^a   Ten Eyck, Lynn F ^a,b   Taylor, Susan S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Apoenzyme; Crystal structure; E230q mutant; Electrostatic surface; Extended N terminal helix

Indexed keywords

ADENOSINE TRIPHOSPHATE MAGNESIUM; APOENZYME; CYCLIC AMP DEPENDENT PROTEIN KINASE; GLUTAMINE; GLYCINE; PROTEIN INHIBITOR; THREONINE;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ELECTRIC ACTIVITY; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SUBUNIT; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; WILD TYPE;

ADENOSINE TRIPHOSPHATE; AMINO ACID SUBSTITUTION; APOENZYMES; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; CYCLIC AMP-DEPENDENT PROTEIN KINASES; GLUTAMIC ACID; GLUTAMINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY;

EID: 27644435575     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.051715205     Document Type: Article

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