A method of mapping protein sumoylation sites by mass spectrometry using a modified small ubiquitin-like modifier 1 (SUMO-1) and a computational program

Molecular and Cellular Proteomics

Volumn 4, Issue 10, 2005, Pages 1626-1636

  Knuesel, Matthew ^a   Cheung, Hiu Tom ^a   Hamady, Micah ^b   Barthel, Kristen K B ^a,b   Liu, Xuedong ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b UCB 450   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; DIGLYCINE; LYSINE; SUMO 1 PROTEIN; UBIQUITIN;

AMINO ACID SUBSTITUTION; ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPUTER PROGRAM; CONTROLLED STUDY; HUMAN; IN VITRO STUDY; INTERNET; MAMMAL; MASS SPECTROMETRY; MUTAGENESIS; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN TARGETING; UBIQUITINATION;

AMINO ACID SEQUENCE; ANIMALS; ARGININE; COMPUTATIONAL BIOLOGY; DNA, COMPLEMENTARY; GTPASE-ACTIVATING PROTEINS; HUMANS; INTERNET; MASS SPECTROMETRY; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTATION; PEPTIDE MAPPING; RABBITS; RETICULOCYTES; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SOFTWARE; SUMO-1 PROTEIN; TIME FACTORS; UBIQUITIN; USER-COMPUTER INTERFACE;

MAMMALIA;

EID: 27644434674     PISSN: 15359476     EISSN: None     Source Type: Journal    
DOI: 10.1074/mcp.T500011-MCP200     Document Type: Article

References (19)

1. Melchior, F. (2000) SUMO - nonclassical ubiquitin. Annu. Rev. Cell Dev. Biol. 16, 591-626
2. Schwartz, D. C., and Hochstrasser, M. (2003) A superfamily of protein tags: ubiquitin, SUMO and related modifiers. Trends Biochem. Sci. 28, 321-328
3. Johnson, E. S. (2004) Protein modification by SUMO. Annu. Rev. Biochem. 73, 355-382
4. Muller, S., Hoege, C., Pyrowolakis, G., and Jentsch, S. (2001) SUMO, ubiquitin's mysterious cousin. Nat. Rev. Mol. Cell. Biol. 2, 202-210
5. Hochstrasser, M. (2000) Biochemistry. All in the ubiquitin family. Science 289, 563-564
6. Denison, C., Rudner, A. D., Gerber, S. A., Bakalarski, C. E., Moazed, D., and Gygi, S. P. (2005) A proteomic strategy for gaining insights into protein sumoylation in yeast. Mol. Cell. Proteomics 4, 246-254
7. Chung, T. L., Hsiao, H. H., Yeh, Y. Y., Shia, H. L., Chen, Y. L., Liang, P. H., Wang, A. H., Khoo, K. H., and Shoei-Lung Li, S. (2004) In vitro modification of human centromere protein CENP-C fragments by small ubiquitin-like modifier (SUMO) protein: definitive identification of the modification sites by tandem mass spectrometry analysis of the isopeptides. J. Biol. Chem. 279, 39653-39662
8. Peng, J., Schwartz, D., Elias, J. E., Thoreen, C. C., Cheng, D., Marsischky, G., Roelofs, J., Finley, D., and Gygi, S. P. (2003) A proteomics approach to understanding protein ubiquitination. Nat. Biotechnol. 21, 921-926
9. Mahajan, R., Gerace, L., and Melchior, F. (1998) Molecular characterization of the SUMO-1 modification of RanGAP1 and its role in nuclear envelope association. J. Cell Biol. 140, 259-270
10. Knuesel, M., Wan, Y., Xiao, Z., Holinger, E., Lowe, N., Wang, W., and Liu, X. (2003) Identification of novel protein-protein interactions using a versatile Mammalian tandem affinity purification expression system. Mol. Cell. Proteomics 2, 1225-1233
11. Pichler, A., Gast, A., Seeler, J. S., Dejean, A., and Melchior, F. (2002) The nucleoporin RanBP2 has SUMO1 E3 ligase activity. Cell 108, 109-120
12. Mahajan, R., Delphin, C., Guan, T., Gerace, L., and Melchior, F. (1997) A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2. Cell 88, 97-107
13. Saitoh, H., Pu, R. T., and Dasso, M. (1997) SUMO-1: wrestling with a new ubiquitin-related modifier. Trends Biochem. Sci 22, 374-376
14. Burch, T. J., and Haas, A. L. (1994) Site-directed mutagenesis of ubiquitin. Differential roles for arginine in the interaction with ubiquitin-activating enzyme. Biochemistry 33, 7300-7308
15. Lois, L. M., and Lima, C. D. (2005) Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1. EMBO J. 24, 439-451
16. Duerksen-Hughes, P. J., Xu, X. X., and Wilkinson, K. D. (1987) Structure and function of ubiquitin: evidence for differential interactions of arginine-74 with the activating enzyme and the proteases of ATP-dependent proteolysis. Biochemistry 26, 6980-6987
17. Walden, H., Podgorski, M. S., and Schulman, B. A. (2003) Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8. Nature 422, 330-334
18. Wohlschlegel, J. A., Johnson, E. S., Reed, S. I., and Yates, J. R., III (2004) Global analysis of protein sumoylation in Saccharomyces cerevisiae. J. Biol. Chem. 279, 45662-45668
19. Deleted in proof