Development of a sandwich-type ELISA for measuring Pla a 1, the major allergen of Platanus acerifolia pollen

International Archives of Allergy and Immunology

Volumn 138, Issue 2, 2005, Pages 127-133

  Arilla, M C ^a   Ibarrola, I ^a   Mir, A ^b,c   Monteseirin J ^d,e   Conde, J ^d   Martinez A ^a   Asturias, J A ^a  

^a Research and Development Department   (Spain)

^b Allergy Service   (Spain)

^c UNIVERSITY OF VALENCIA   (Spain)

^d HOSPITAL UNIVERSITARIO VIRGEN MACARENA   (Spain)

^e Sagrado Corazón Clinic   (Spain)

Author keywords

Pla a 1; Planetree; Platanus acerifolia; Two site sandwich ELISA

Indexed keywords

BIOTIN; FOOD ALLERGEN; GRASS POLLEN EXTRACT; HOUSE DUST ALLERGEN; IMMUNOGLOBULIN E ANTIBODY; MONOCLONAL ANTIBODY; POLLEN ANTIGEN; POLYCLONAL ANTIBODY;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY LABELING; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ANTIGEN DETECTION; ANTIGEN PURIFICATION; ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; DRUG PURIFICATION; DRUG SENSITIVITY; DRUG SPECIFICITY; DRUG SYNTHESIS; DRUG USE; ENZYME IMMUNOASSAY; FEMALE; IMMUNIZATION; ION EXCHANGE CHROMATOGRAPHY; LINEAR SYSTEM; MOULD; MOUSE; NONHUMAN; PLATANUS ACERIFOLIA; POLLEN ALLERGY; PRIORITY JOURNAL; PROTEIN CONTENT; QUANTITATIVE ANALYSIS; RABBIT; STANDARDIZATION; TREE;

ALLERGENS; ANGIOSPERMS; ANIMALS; ANTIBODIES, MONOCLONAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN E; PLANT EXTRACTS; POLLEN; RABBITS;

EID: 27544485366     PISSN: 10182438     EISSN: None     Source Type: Journal    
DOI: 10.1159/000088434     Document Type: Article

References (23)

1. Subiza J, Cabrera M, Valdivieso R, Subiza JL, Jerez M, Jiménez JA, Narganes MJ, Subiza E: Seasonal asthma caused by airborne Platanus pollen. Clin Exp Allergy 1994;24:1123-1129.
2. Valero AL, Rosell E, Amat P, Sancho J, Roig J, Piulats J, Malet A: Hipersensibilidad a polen de Platanus acerifolia: detección de las fracciones alergénicas. Alergol Inmunol Clin 1999;14:220-226.
3. Subiza J: Pólenes alergénicos en España. Allergol Immunopathol 2004;32:121-124.
4. Enrique E, Cisteró-Bahíma A, Bartolomé B, Alonso R, San Miguel-Moncín MM, Bartra J, Martínez A: Platanus acerifolia pollinosis and food allergy. Allergy 2002;57:351-356.
5. Miralles JC, Caravaca F, Guillén F, Lombardero M, Negro JM: Cross-reactivity between Platanus pollen and vegetables. Allergy 2002;57:146-149.
6. Enrique E, Alonso R, Bartolomé B, San Miguel-Moncín MM, Bartra J, Fernández-Parra B, Tella R, Asturias JA, Ibarrola I, Martínez A, Cisteró-Bahíma A: IgE reactivity to profilin in Platanus acerifolia pollen-sensitized subjects with plant-derived food allergy. J Invest Allergol Clin Immunol 2004;14:335-342.
7. Asturias JA, Ibarrola I, Bartolomé B, Ojeda I, Malet A, Martínez A: Purification and characterization of Pla a 1, a major allergen from Platanus acerifolia pollen. Allergy 2002;57:221-227.
8. Asturias JA, Ibarrola I, Eraso E, Arilla MC, Martínez A: The major Platanus acerifolia pollen allergen Pla a 1 has sequence homology to invertase inhibitors. Clin Exp Allergy 2003;33:978-985.
9. Ibarrola I, Arilla MC, Martínez A, Asturias JA: Identification of a polygalacturonase as a major allergen (Pla a 2) from Platanus acerifolia pollen. J Allergy Clin Immunol 2004;113:1185-1191.
10. van Ree R, Kurth R, Haustein D (eds): Regulatory Control and Standardization of Allergenic Extracts. A New Start for Allergen References and Standardization Based on Purified (Natural or Recombinant) Allergens and Monoclonal and Monospecific Polyclonal Antibodies. Darmstadt, GIT Verlag, 2000, pp 87-92.
11. García Ortega P, Martínez J, Martínez A, Palacios R, Belmonte J, Richart C: Mercurialis annua pollen: a new source of allergic sensitization and respiratory disease. J Allergy Clin Immunol 1992;89:987-993.
12. American Society for Testing and Materials (ASTM): Standard Test Method for the analysis of aqueous extractable protein in natural rubber and its products using the modified Lowry method (ASTH DS712-99). Annual Book of ASTM Standards, 1999, vol 09.02.
13. Ceska M, Lundkvist U: A new and simple radioimmunoassay method for the determination of IgE. Immunochemistry 1972;9:1021-1030.
14. Arilla MC, Asturias JA, Gómez-Bayón N, Martínez A, Martínez J, Palacios R: Production and characterization of profilin monoclonal antibodies. Allergol Immunopathol 1997;25:145-151.
15. Köhler G, Milstein C: Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol 1976;6:511-519.
16. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;277:680-685.
17. van Ree R: The create project: EU support for the improvement of allergen standardization in Europe. Allergy 2004;59:571-574.
18. Schmidt M, Hoffman DR: Expression systems for production of recombinant allergens. Int Arch Allergy Immunol 2002;128:264-270.
19. Varela S, Subiza J, Subiza JL, Rodríguez R, García B, Jerez M, Jiménez JA, Panzani R: Platanus pollen as an important cause of pollinosis. J Allergy Clin Immunol 1997;100:748-754.
20. Bousquet J, Lockey R, Malling HJ: Allergen immunotherapy: therapeutic vaccines for allergic diseases. Allergy 1998;53:1-42.
21. Durham SR, Walker SM, Varga EM, Jacobson MR, O'Brien F, Noble W, Till SJ, Hamid QA, Nouri-Aria KT: Long-term clinical efficacy of grass-pollen immunotherapy. N Engl J Med 1999;341:468-475.
22. Arilla MC, Ibarrola I, García R, de la Hoz B, Martínez A, Asturias JA: Quantification of the major allergen from cypress (Cupressus arizonica) pollen, Cup a 1, by monoclonal antibody-based ELISA. Int Arch Allergy Immunol 2004;134:10-16.
23. Jimeno L, Duffort O, Serrano C, Barber D, Polo F: Monoclonal antibody-based ELISA to quantify the major allergen of Artemisia vulgaris pollen, Art v 1. Allergy 2004;59:995-1001.