Protein separation on a technical scale using a radial symmetric free flow zone electrophoresis cell

Chemical Engineering Science

Volumn 60, Issue 23, 2005, Pages 6574-6583

  Melin, Thomas ^a   Poggel, Martin ^a,b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b NONE   (Germany)

Author keywords

Electrophoresis: Preparative scale; Protein; Scale up: Downstream processing; Separation

Indexed keywords

BIOCHEMISTRY; ELECTRIC FIELDS; ELECTROPHORESIS; HEATING; PROTEINS; SEPARATION; TURBULENCE;

ELECTROPHORESIS: PREPARATIVE SCALE; FLOW PATTERNS; PROTEIN SEPARATION; SCALE-UP: DOWNSTREAM PROCESSING;

FLUID DYNAMICS;

ELECTROPHORESIS; FLOW PATTERN; PROTEIN; SEPARATION;

EID: 27444444280     PISSN: 00092509     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ces.2005.05.060     Document Type: Article

References (20)

1. AEA Technology, 1997. CFX 4.2-Solver Manual. Harvell.
2. Bailey, J.E., Ollis, D.F., 1986. Biochemical Engineering Fundamentals. second ed. McGraw-Hill Inc., New York. p. 746.
3. Baygents, J.C., Baldessari, F., 1998. Electrohydrodynamic instability in a thin layer with an electrical conductivity gradient. Physics of Fluids 10 (1), 301-311.
4. Clifton, M.J., Afonso, J.L., 1998. Numerical model of a mixed flow coupled with mass and heat transfer in continuous-flow electrophoresis. Computers and Chemical Engineering 22, 323.
5. Douglas, N.G., Humffray, A.A., Pratt, H.R.C., Stevens, G.W., 1995. Electrophoretic mobilities of proteins and protein mixtures. Chemical Engineering Science 50 (5), 743-754.
6. Freitag, R., Horváth, C., 1996. Chromatography in downstream processing of biotechnological products. In: Fiechter, A. (Ed.), Advances in Biochemical Engineering/Biotechnology, vol. 53. Springer, Berlin, Heidelberg, pp. 17-59.
7. Greenlee, R.D., Ivory, C.F., 1998. Protein focusing in a conductivity gradient. Biotechnology Progress 14, 300-309.
8. Hannig, K., Heidrich, H.G., 1990. Free-flow Electrophoresis. GIT Verlag, Darmstadt.
9. Heinrich, J., Wagner, H., 1993. Influence of the electrode compartment separating membranes on continuous flow electrophoresis. Electrophoresis 14, 99-107.
10. Huisman, I.H., Trägardh, G., 1999. Determining the zeta potential of ultrafiltration membranes using their salt retention. Colloid and Surfaces A 157, 261-268.
11. Mattock, P., Aitchison, G.F., Thomson, A.R., 1980. Velocity gradient stabilized, continuous free flow electrophoresis (a review). Separation Purification Methods 9 (1), 1-68.
12. Nath, S., Schuette, H., Hustedt, H., Deckwer, W.D., Weber, G., 1996. Separation of enzymes from microorganism crude extracts by free-flow zone electrophoresis. Biotechnology and Bioengineering 51, 15-22.
13. O'Connor, A.J., Pratt, H.R.C., Stevens, G.W., 1996. Electrophoretic mobilities of proteins and protein mixtures in porous membranes. Chemical Engineering Science 51 (13), 3459-3477.
14. Philpot, J.St.L., 1973. In: Reid, E. (Ed.), Methodological Developments in Biochemistry, vol. 2, Preparative Techniques, Longmans, England, pp. 81-85.
15. Poggel, M., 2004. Entwicklung kontinuierlich arbeitender Elektrophorese-Anlagen zur präparativen Proteinaufbereitung. Ph.D. Thesis, RWTH Aachen, Germany, Shaker Verlag, Aachen.
16. Poggel, M., Melin, T., 2001. Free-flow zone electrophoresis: a novel approach and scale-up for preparative protein separation. Electrophoresis 22, 1008-1015.
17. Poggel, M., Melin, T., 2002. Transport phenomena in free flow zone electrophoresis. Engineering Life Science 2 (10), 298-304.
18. Poggel, M., Melin, T., Treutlein, S., 2002. Band-broadening effects in preparative free flow zone electrophoresis. Electrophoresis 23, 2252-2258.
19. Rhodes, P.H., Snyder, R.S., Roberts, G.O., 1989. Electrohydrodynamic distortion of sample streams in continuous flow electrophoresis. Journal of Colloid and Interface Science 129 (1), 78-90.
20. Szymczyk, A., Fievet, P., Mullet, M., Reggiani, J.C., Pagetti, J., 1998. Study of electrokinetic properties of plate ceramic membranes by electroosmosis and streaming potential. Desalination 119, 309-314.