EPR evidence for chromium(V) binding to phosphate and pyrophosphate: Implications for chromium(V)-DNA interactions

Inorganic Chemistry

Volumn 35, Issue 13, 1996, Pages 3727-3728

  Sugden, Kent D ^a   Wetterhahn, Karen E ^a  

^a Dartmouth College   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 2742554395     PISSN: 00201669     EISSN: None     Source Type: Journal    
DOI: 10.1021/ic960199+     Document Type: Article

References (20)

1. Liebross, R. H.; Wetterhahn, K. E. Chem. Res. Toxicol. 1990, 3, 401-403.
2. Sugiyama, M.; Tsuzuki, K., Hidaka, T.; Ogura, R.; Yamamoto, M. Biol. Trace Elem. Res. 1991, 30, 1-8.
3. Witmer, C.; Faria, E.; Park, H.-S.; Sadrieh, N.; Yurkow, E.; O'Connell, S.; Sirak, A.; Schleyer, H. Environ. Health Perpect. 1994, 102, 169-176.
4. Jiang, J.; Liu, K. J.; Shi, X.; Swartz, H. M. Arch. Biochem. Biophys. 1995, 319, 570-573.
5. Farrell, R P ; Judd, R. J.; Lay, P. A.; Dixon, N. E.; Baker, R. S. U., Bonin, A. M. Chem. Res. Toxicol. 1989, 2, 227-229.
6. Stearns, D. M.; Kennedy, L. J ; Courtney, K. D ; Giangrande, P. H.; Phieffer, L. S : Wetterhahn, K E. Biochemistry 1995, 34, 910-919.
7. Abdrakhmanov, R. S.; Garif' yanov, N. S.; Semenova, E. I. RUSS. J. Inorg. Chem. (Engl. Transl.) 1968, 13, 1260-1262.
8. Marov, I. N.; Abramova, T. I.; Ermakov, A. N. Russ. J. Inorg. Chem. (Engl. Transl.) 1969, 14, 1268-1270.
9. Synthesized by the method of: Krumpolc, M.; Rocek, J. J. Am. Chem. Soc. 1979, 101, 3206-3209.
10. 5 receiver gam, 2 mW microwave power attenuated at 20 dB, 3350-3450 G sweep width, and a 21 s scan time. RT parameters were identical except for a 9.769-9.772 GHz microwave frequency, and 3480-3580 G sweep width. All signals were averaged over nine scans. Measurements were done on ca. 100 μL volume samples drawn into a capillary tube that was sealed on one end with Dow-Corning high-vacuum grease. The g values were determined with respect to the 2,2-diphenyl-lpicrylhydrazyl radical (DPPH), g = 2.0036.
11. -.
12. Symons, M. Chemical and Biochemical Aspects of Electron-Spin Resonance Spectrascopy; John Wiley and Sons: New York, 1978.
13. Farrell, R. P.; Lay, P. A. Comments Inorg. Chem. 1992, 13, 133-175.
14. See: Holm, R H. Chem. Rev. 1987, 87, 1401-1449 and references therein.
15. Bramley, R.; Ji. J.-Y.; Lay, P A. Inorg. Chem. 1991, 30, 1557-1564.
16. Farrell, R. P.; Judd, R. J.; Lay, P. A.; Bramley, R.: Ji, J -Y. Inorg. Chem. 1989, 28, 3401-3403.
17. Bramley, R.; Farrell, R. P.; Ji, J.-Y : Lay, P. A. Inorg. Chem. 1990. 29, 3089-3094.
18. Several reviewers have questioned the biological relevance of the phosphate and pyrophosphate concentrations used in this study. Estimates of DNA - phosphate concentrations are 5-10 mM in eukaryotic cells (Lodish, H.; Baltimore, D.; Berk, A.; Zipursky, S. L ; Matsudaira. P.; Darnell, J. Molecular Cell Biology, 3rd ed.; W H. Freeman and Co.: New York, 1995), while the concentration of a single nucleotide, ATP. has been estimated at 1-5 mM in eukaryotic cells (Toy, A. D F.; Walsh, E. N. Phosphorus Chemistry in Everyday Living; American Chemical Society: Washington, DC. 1987). When the concentrating effects of cellular compartmentalization, e.g. DNA localization at the nucleus, are taken into account, the 100-500 mM phosphate and pyrophosphate concentrations used in this study are not particularly high.
19. Several reviewers have questioned the biological relevance of the phosphate and pyrophosphate concentrations used in this study. Estimates of DNA - phosphate concentrations are 5-10 mM in eukaryotic cells (Lodish, H.; Baltimore, D.; Berk, A.; Zipursky, S. L ; Matsudaira. P.; Darnell, J. Molecular Cell Biology, 3rd ed.; W H. Freeman and Co.: New York, 1995), while the concentration of a single nucleotide, ATP. has been estimated at 1-5 mM in eukaryotic cells (Toy, A. D F.; Walsh, E. N. Phosphorus Chemistry in Everyday Living; American Chemical Society: Washington, DC. 1987). When the concentrating effects of cellular compartmentalization, e.g. DNA localization at the nucleus, are taken into account, the 100-500 mM phosphate and pyrophosphate concentrations used in this study are not particularly high.
20. Bianchi, V; Celotti, L.; Lanfranchi, G.; Majone, F.; Marin, G. Montaldi, A.; Sponza, G.; Tamino, G.; Venier, P ; Zantedeschi, A ; Levis, A. G. Mutat. Res. 1983, 117, 279-300.