Effect of polymer matrix and glycerol on rapid single-strand conformation polymorphism analysis by capillary and microchip electrophoresis for detection of mutations in K-ras gene

Electrophoresis

Volumn 26, Issue 17, 2005, Pages 3380-3386

  Endo, Yuki ^a,b   Zhang, Lihua ^a,c   Katashima, Rumi ^a,g,h   Itakura, Mitsuo ^a   Doherty, Erin A S ^d   Barron, Annelise E ^d   Baba, Yoshinobu ^a,b,e,f  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c FURUNO ELECTRIC CO LTD   (Japan)

^d Northwestern University   (United States)

^e NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^f NAGOYA UNIVERSITY   (Japan)

^g Koto ku   (Japan)

^h Applied Biosystems Japan   (Japan)

Author keywords

Capillary electrophoresis; Microchip electrophoresis; Mutation detection; Single strand conformation polymorphism

Indexed keywords

GENES; MICROPROCESSOR CHIPS; POLYMORPHISM;

K-RAS GENE; MICROCHIP ELECTROPHORESIS; MUTATION DETECTION; OPTIMAL CONDITIONS; POLYMER MATRICES; POLYMORPHISM ANALYSIS; SIEVING MATRIX; SINGLE-STRAND CONFORMATION POLYMORPHISM; STRAND CONFORMATION; WILD TYPES;

CAPILLARY ELECTROPHORESIS;

BUFFER; DNA FRAGMENT; GLYCEROL; K RAS PROTEIN; POLYMER; SINGLE STRANDED DNA; PRIMER DNA;

ACCURACY; ARTICLE; CAPILLARY ELECTROPHORESIS; ELECTRIC FIELD; GENE AMPLIFICATION; GENE MUTATION; HUMAN; MICROPROCESSOR; MUTATIONAL ANALYSIS; SINGLE STRAND CONFORMATION POLYMORPHISM; TIME; WILD TYPE; METHODOLOGY; MICROCHIP ELECTROPHORESIS; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; ONCOGENE RAS; POLYMERASE CHAIN REACTION;

BASE SEQUENCE; DNA PRIMERS; ELECTROPHORESIS, CAPILLARY; ELECTROPHORESIS, MICROCHIP; GENES, RAS; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL;

EID: 27244451339     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200500142     Document Type: Article

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