Structural arrangement of DNA constrained by a cross-linker

Organic and Biomolecular Chemistry

Volumn 3, Issue 19, 2005, Pages 3476-3478

  Endo, Masayuki ^a   Majima, Tetsuro ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CROSSLINKING; GELS; MELTING; NITROGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; STEREOCHEMISTRY; SYNTHESIS (CHEMICAL);

CIRCULAR DICHROISM SPECTRA; GEL MOBILITY; MELTING PROFILES;

DNA;

AMIDE; CROSS LINKING REAGENT; DISULFIDE; DNA; OLIGONUCLEOTIDE; PHOSPHORAMIDIC ACID; PHOSPHORIC ACID;

ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; CONFORMATION; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; STEREOISOMERISM; SYNTHESIS; TEMPERATURE;

AMIDES; BASE SEQUENCE; CIRCULAR DICHROISM; CROSS-LINKING REAGENTS; DISULFIDES; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; NUCLEIC ACID CONFORMATION; OLIGONUCLEOTIDES; PHOSPHORIC ACIDS; STEREOISOMERISM; TEMPERATURE;

EID: 27144464160     PISSN: 14770520     EISSN: None     Source Type: Journal    
DOI: 10.1039/b507126g     Document Type: Article

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25. p-configuration) via the minor groove.
26. Cleavage of the disulfide linkage of oligonucleotides was carried out in a solution containing 10 mM dithiothreitol (DTT) and 50 mM Tris-HCl (pH 8) at 50°C for 30 min. Thiol-tethered DNAs were purified by HPLC. Then, the thiol-tethered DNA was treated with an excess of 5,5′-bis(thio-2- nitrobenzoic acid) (DTNB) in 50 mM Tris-HCl (pH 8) for 2 h and purified by HPLC. An equal amount of the thiol-tethered DNA was added to the TNB-activated DNA in 50 mM Tris-HCl (pH 8) buffer at rt for 12 h, and the resulting cross-linked DNAs were purified by HPLC.
27. 2 6199.3 [M-H] ; found, 2A: 6199.4; 2B: 6199.4.
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31. -1. Measurements were carried out in a 1 mL solution containing 2.0 μM DNA. 10 mM Tris-HC1 (pH 7.6), and 1.0 M NaCl.