Frozen storage of ram semen I. Processing, freezing, thawing and fertility after cervical insemination

Animal Reproduction Science

Volumn 37, Issue 3-4, 1995, Pages 185-249

  Salamon, S ^a   Maxwell, W M C ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Artificial insemination; Cold storage; Reproduction; Semen; Sheep male

Indexed keywords

EID: 27044443915     PISSN: 03784320     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-4320(94)01327-I     Document Type: Review

References (375)

1. Freezing of ram semen in straws
2. Artificial insemination of ewes using frozen semen
3. Studies on the presence and absence of glycerol in unfrozen and frozen semen: fertility trials and the effect of dilution methods on freezing ram semen in the absence of glycerol
4. Studies on the absence of glycerol in unfrozen and frozen ram semen
5. Criopreservación de semen ovino
6. Artificial insemination with frozen semen in sheep in Norway
7. Intra-uterine and deep cervical insemination with frozen semen in sheep
8. Fertility and fecundity of ewes inseminated with deep-frozen semen under the conditions of South-East Kazakhstan (in Russian)
9. Some advances in cryopreservation of ram semen (in Russian)
10. Untersuchungen zum Einfluss der Verdünnung, Äquilibrierung und des Auftauens von Schafbocksperma bei dessen Tiefgefrierkonservierung nach dem Pelletverfahren
11. Künstliche Besamung (KB) mit Gefriersperma beim Schaf-Inseminationspunkt und ein-oder zweimalige Besamung
12. The survival of frozen bovine spermatozoa following minimum exposure to glycerol
13. The freezability of spermatozoa after minimal pre-freezing exposure to glycerol or lactose
14. Effect of non-electrolytes on viability of spermatozoa (in Russian)
15. Deep freezing of semen from Askanian rams (in Russian)
16. Factors affecting the revival of bull and ram spermatozoa after freezing to −70°C
17. The effects of pH and the temperature of glycerolization on the revival of ram and bull spermatozoa after freezing to −79°C
18. The effects of milk diluents on the viability of ram spermatozoa and their revival after freezing
19. Survival of deep-frozen mammalian spermatozoa
20. Freezing ram semen in ampoules to −196°C (in Rumanian)
21. Freezing of ram semen at low temperatures. I. Current opinions on conservation at low temperatures
22. Freezing of ram semen at low temperatures. II. Diluent trials
23. Damage to spermatozoa at thawing and methods of avoiding it (in Russian)
24. Acción del sulfóxide de dimetilo y glicerol como agentes crioprotectores del acrosoma del espermatozoide de carnero durante la congelación
25. Inseminacion con semen congelado de carnero
26. Preservation of bull and ram semen after thawing, by redilution
27. A study on the freezing of sheep semen (in Mandarin with English summary)
28. Fertilité de brebis inséminées aves du sperme frais ou congelé après traitment progestatif, pendant la saison sexuelle
29. Effect of initial freezing temperature, addition of glycerol and dilution on the survival and fertilizing ability of deep-frozen ram semen
30. The use of progestagen SC9880 as an aid for artificial insemination in ewes
31. Fertility in the ewe after artificial insemination with fresh and frozen semen at the induced oestrus, and influence of the photoperiod on the semen quality of the ram
32. Fertilité des brebis traitées avec de l'acétate de fluorogestone et inséminées artificiellement avec du sperme congelé resultats préliminaires
33. Etude des principaux facteurs de survie et de fecondance du sperme de belier après congélation
34. Seasonal variations of the fertilizing capacity of deep-frozen ram semen
35. A new method for thawing frozen semen
36. L'insemination artificielle dans le plans d'intensification de la production ovine; état actuel et perspectives
37. Investigations on the effect of different cryobiological factors on the quality and fertilizing ability of frozen ram semen
38. Changes in the ultrastructure of ram spermatozoa due to freezing. 1. The effect of the Triladil and saccharose-based semen diluents
39. Quelques résultats sur l'insémination artificielle des brebis et des chèvres en France
40. Livability of bovine spermatozoa at 5 −25 and −85°C in Tris-buffered and citrate-buffered yolk-glycerol
41. Minimal time required in contact with glycerol for cryopreservation of ram semen
42. Recherches sur la vitalité des spermatozoides de quelques poissons d'eau douce
43. Comparison between natural service and artificial insemination with fresh or frozen sperm at an appointed time in the ewe
44. A diluent for freezing of ram semen
45. The low temperature storage of ram, bull and rabbit spermatozoa
46. The fertility of frozen ram and bull semen
47. Artificial insemination of sheep
48. Effects of composition of diluent, method of addition of glycerol, freezing rate, and storage temperature on the revival of ram spermatozoa after deep-freezing
49. Freezing ram semen in a diluent containing dried egg yolk
50. Use of sulphagone at insemination of ewes
51. Peroxidation of lipids in ram semen
52. The relevance of cryoprotectant toxicity to cryobiology
53. Mineral changes in ram spermatozoa as affected by freezing and various extenders
54. Effects of interaction between cooling and rewarming conditions on survival of cells
55. Studies on conservation of ram semen by freezing to −79°C (in Rumanian)
56. Efecto del tipo de diluyente y método de congelación sobre la fertilidad del semen ovino
57. The influence of glycerol and various diluents on low temperature survival of ram spermatozoa
58. The motility decline of frozen ram semen during prolonged −79°C storage and the effect of glycerol equilibration on motility of thawed frozen ram semen incubated at 39°C and 5°C
59. The addition of egg yolk to a milk dilutor and its effect on glycerol requirement for freezing ram semen
60. The frozen storage of ram semen
61. Fertility of frozen and unfrozen ram semen
62. Computerized videomicrography applied to deep-frozen ram semen, with reference to osmolarity of the diluent and morphology of the spermatozoal head cap
63. New extenders for freezing ram semen
64. Effect of equilibration time at 5°C and photoperiod on survival of ram spermatozoa frozen in straws
65. The effect of glycerol concentration and cooling velocity on cryosurvival of ram spermatozoa frozen in straws
66. The effects of rapid cooling, cold shock of ram semen, photoperiod, and egg yolk in diluents on the survival of spermatozoa before and after freezing
67. The effect of glycerol-related osmotic changes on post-thaw motility and acrosomal integrity of ram spermatozoa
68. Effect of osmolarity of skim-milk diluents and thawing rate on cryosurvival of ram spermatozoa
69. Cryosurvival of ram spermatozoa in hypertonic and isotonic diluents
70. Evaluation of a new diluent and different processing procedures for cryopreservation of ram semen
71. Effect of long-term storage diluents on the acrosomes of ram spermatozoa
72. Progress in the artificial insemination of sheep with frozen semen
73. Effect of different diluents thawing temperatures and materials of thawing containers on survival of ram spermatozoa frozen by the pellet method
74. The storage of ram semen at low temperatures
75. Physiologische Untersuchungen an Spermatozoen und Eiern
76. Changes of some biochemical and biological characteristics of ram spermatozoa during freezing in different media
77. Hydrogen ion buffers for biological research
78. The fertility of frozen ram semen stored at −79°C (in Portugese)
79. Current status of semen preservation in the ram, boar and stallion
80. The effect of antioxidants on frozen ram semen (in Russian)
81. Deep freezing of ram semen (in Russian)
82. Artificial insemination with frozen ram semen in Norway
83. Influence of some cryoprotectors on the ATP concentration in ram spermatozoa at different stages of cryoconservation
84. 4 A 61 D 7/02
85. Contribution to the deep freezing-preservation of goat-buck and ram semen
86. Field evaluation of a technique for transcervical intrauterine insemination of ewes
87. Use of spin labels and electron spin resonance spectroscopy to characterize membranes of bovine sperm: effect of butylated hydroxytoluene and cold shock
88. Use of spin labels to evaluate effects of cold shock and osmolarity on sperm
89. Effect of glycerol equilibration time, glycerol level, and rate of temperature descent on the freezing of ram spermatozoa
90. Effects of diluent, washing and equilibration time on progressive motility and glutamic oxaloacetic transaminase levels for frozen ram semen
91. Revival of mammalian sperm after immersion in liquid nitrogen
92. Observations on some factors influencing vitality of ram semen during its freezing and thawing
93. Pregnancy rates following intra-uterine insemination with pellet or straw-frozen ram semen
94. Frozen storage of ram spermatozoa in straws
95. Pregnancy rates following intra-uterine insemination with pellet or straw-frozen ram semen
96. Intra-uterine insemination with pellet or straw-frozen ram semen
97. The use of liposomes to stabilise sperm membranes during freezing
98. The effect of electrolytes in sugar-egg yolk media on the quality of frozen ram semen
99. Arch. Sci. Biol. Inst. Imp. Med. Exp. St. Pétersbourg
100. Über die Wiederstandsfähigkeit von menschlichen Spermatozoen gegenüber starker
101. Optima of glycerol, tris and thaw rate in freezing ram semen
102. The use of dimethyl sulphoxide, glycerol, and reconstituted skim milk for the preservation of ram spermatozoa. II. The influence of diluent composition and processing time during freezing to −79°C with dimethyl sulphoxide or glycerol or both compounds
103. Studies of the suitability of preparations of ewe and cow milk for storing ram spermatozoa at 37, 5 and −79°C
104. Influence of diluents and processing times after ejaculation on the survival of deep-frozen ram spermatozoa
105. Lipid peroxides in spermatozoa; formation, role of plasmalogen, and physiological significance
106. Toxicity of exogenous fatty acid peroxides towards spermatozoa
107. Damage to ram spermatozoa by peroxidation of endogenous phospholipids
108. Deep-freezing ram spermatozoa the effects of milk yolk-citrate and synthetic diluents containing sugar
109. The effects of dilution, egg yolk and cooling to 5°C on the ultrastructure of ram spermatozoa
110. The effect of thawing regime on the motility of frozen spermatozoa (in Russian)
111. All-year-round use of Karakul rams
112. Sur la méthode de congélation profonde du sperme du taureau, de bélier et de bouc
113. Insemination of ewes with deep frozen semen
114. Insemination of ewes with semen frozen in different media
115. Recent research on freezing ram semen
116. Insemination of ewes with frozen semen
117. The freezing of ram semen at low temperatures. III. The fertility of ewes after insemination with frozen semen
118. Fertility of frozen ram semen diluted in citrate buffer with or without added bull seminal plasma
119. Fertility and conception rate of sheep inseminated with deep-frozen semen
120. A progressive method of storing semen
121. Fertilising capacity of deep-frozen ram spermatozoa
122. Freezing semen at −21°C
123. Evaluation of butylated hydroxytoluene as a cryopreservative added to whole or skim milk diluent for bull semen
124. Freezing and use of ram semen
125. Storage of ram semen at −78° temperature
126. Tiefgefrierung von Schafbocksperma in Pelletform mit unterschieldlichen Abkühlverfahren vor dem Einfrieren
127. Observations on the viability of spermatozoa of animals
128. Preservation of ram semen at a temperature of −79°
129. Survival of ram spermatozoa frozen in pellets, straws and minitubes
130. A comparison of fresh and frozen semen in the insemination of confined sheep
131. Influence of estradiol 17-β on fertility in confined sheep inseminated with frozen semen
132. Preservation of spermatozoa
133. Measurements of cooling rates and cell survival at different points within a sphere
134. Freezing of ram semen by the pellet method. II. The effect of method of dilution, dilution rate, glycerol concentration, and duration of storage at 5°C prior to freezing on survival of spermatozoa
135. Freezing of ram semen by the pellet method. III. The effect of pellet volume, composition of the thawing solution, and reconcentration of the thawed semen on survival of spermatozoa
136. Fertility of ram spermatozoa frozen by the pellet method. II. The effects of method of insemination on fertilization and embryonic mortality
137. Lambing rates after single inseminations of ewes with liquid or deep-frozen semen
138. Field trials with frozen semen
139. Experiments on freezing ram semen
140. Causes of low fertility of ewes inseminated with frozen semen (in Russian)
141. Improvement of storage technique for ram semen and methods of increasing the fertility of ewes
142. Experimental test of different methods of freezing ram semen (in Russian)
143. Evaluation of different methods of freezing semen (in Russian)
144. Viability of frozen ram spermatozoa in different parts of the genital tract
145. Storage of semen at −183° temperature
146. Method of freezing ram semen at temperatures of −183 or −196°
147. Laboratory and field investigations of the method of freezing ram semen to −196°
148. Possibility of freezing semen without glycerol
149. A method of freezing animal semen in media without glycerol and egg yolk
150. Life is preserved at −196° below zero temperature
151. Method of freezing ram semen
152. Quelques aspects concernant la congélation du sperme de belier
153. Factors in the revival of Anguillula aceti after its solidification in liquid air
154. Revival of frog's spermatozoa vitrified in liquid air
155. Storage and use of frozen bull and ram semen
156. Use of a complex medium with echinochrome for insemination of ewes (in Russian)
157. Fisiologia sullo sperma umano
158. Acrosomal proteolytic activity as a method for evaluation of the cryoprotective action of diluents for freezing of ram semen
159. Semen diluents for freezing ram spermatozoa (in Bulgarian)
160. Investigation on the effect of diluents containing antioxidants on the freezability of ram spermatozoa (in Bulgarian)
161. Effect of freezing regime on viability of ram spermatozoa (in Bulgarian)
162. Examination of the effect of some factors on freezing ram semen (in Bulgarian)
163. Some data on preservation and freezing of ram semen
164. The viability of diluted ram spermatozoa at 37°C, 5°C and −79°C
165. Artificial insemination of sheep: effects on fertility of number of spermatozoa inseminated and of storage of diluted semen for up to 18 hours at 5°C
166. Pellet freezing of ram semen
167. Cryopreservation of ram semen in egg yolk lactose raffinose citrate glycerol extender
168. Effect of incorporation of tris in “EYLRCG” extender for straw freezing of ram spermatozoa
169. Effect of various cryobiological factors on the quality and fertility of frozen sheep semen
170. Einfluss verschiedener kryobiologischer Faktoren auf die Qualität und Befruchtungsfähigkeit von tiefgefrorenem Schafsperma
171. Contribution to the problem of freezing plants
172. Fertility of ram semen frozen in autumn and spring
173. Liquid storage of ram semen
174. Fertility of ewes following artificial insemination with semen frozen in pellets or straws a preliminary report
175. Causes of injury in frozen and thawed cells
176. The role of intracellular freezing in the death of cells cooled at supraoptimal rates
177. Basic concepts in freezing cells
178. Deep freezing and long-term storage of ram semen (in Russian)
179. Determinación de la temperatura y tiempo óptimos para la descongelación del semen de ovino
180. In-vitro und in-vivo-Untersuchungen zur Tieftemperatur Konservierung von Schafbocksperma
181. Die Kaltbesamung mit Kapselpellets praxisreif
182. Cryoprotective agents
183. Survival of ram spermatozoa using different diluents and deep freezing in liquid nitrogen
184. Preserving ram semen
185. Fertility of ewes inseminated with deep-frozen semen
186. Effect of special nutrition of rams on the lipid content of spermatozoa and on fertility results after insemination
187. Long-term storage of ram semen and new possibilities of large-scale selection in sheep breeding
188. The results and perspectives of scientific research
189. Cryogenic damage to ram spermatozoa
190. Lyzophosphatides and their significance in the preservation of bull and ram semen
191. Method of deep freezing of ram semen for long-term storage
192. Long-term storage and long-distance transport of ram semen
193. Chemical characteristics of the antioxidants and their effect during freezing of ram semen
194. Use of echinochrome in the artificial insemination of ewes
195. Medium for deep-freezing of semen
196. An investigation of cryoprotective diluents for pellet-freezing ram spermatozoa
197. Effect of mono- and di-saccharides in tris-based diluents on motility and fertility of pellet-frozen ram spermatozoa
198. Further evaluations of zwitterion buffers in freezing diluents for ram spermatozoa
199. The effect of low temperatures on structural characters of acrosomes of ram spermatozoa
200. Preservation of ram semen in a frozen state
201. Preservation of ram semen in a frozen state by using hypertonic diluents
202. Elaboration of the problems of preservation of semen of farm animals in a frozen state
203. Preservation of ram semen in a frozen state
204. Pelleted semen: comparison of different extenders and processes on fertility of bovine spermatozoa
205. Deep freezing of bull semen in concentrated pellet form. II. Protective action of sugars
206. Protective effects of sugars against freezing injury of bull spermatozoa
207. Introductory remarks
208. Effect of the processing technique on the permeability of sperm membranes and stability of protein-lipid complexes
209. Change in membrane permeability and resistance of protein-cholesterol complexes under the influence of technological processing of semen
210. Effectiveness of antioxidants in freezing of ram semen
211. Synthetic media for preservation of ram semen at − 196°C
212. Biochemical character of the low resistance of ram spermatozoa against deep freezing
213. Cryogenic changes in ram spermatozoa
214. Aerodynamical thawing of semen
215. Befruchtungsergebnisse beim Schaf nach Insemination gefrierkonservierten Spermas unter besonderer Berücksichtigung einer intrauterinen transmuralen Deponierung
216. Gefrierkonservierung von Schafbockspermien in Gegenwart oberflächenaktiver Substanzen
217. Probleme der Tieftemperaturkonservierung von Schafbockspermien. 1. Mitteilung: Der Einfluss verschiedener Kryoprotektiva auf die Spermien
218. Probleme der Tieftemperaturkonservierung von Schafbockspermien. 2. Mitteilung: Der Einfluss des Bockes und der Taugeschwindigkeit auf die Trächtigkeitsrate sowie Untersuchungen zur Fertilitätsprognose
219. Artificial insemination in sheep with particular reference to the deep freezing of semen
220. Intrauterine insemination in ewes with frozen semen
221. Insemination of sheep with frozen semen
222. The influence of β-glucuronidase on survival and fertilizing ability of frozen ram semen
223. Technique, organisation and results of artificial insemination of cows and ewes with frozen semen
224. The action of temperature shock on the quality of frozen semen
225. Technology of aseptic production and cryopreservation of sperm according to Kharkon method
226. Ram sperm freezing in film package
227. Preservation of human spermatozoa at low temperatures
228. Effects of cryopreservation procedures on sperm membranes
229. Effects of diluents, equilibration time and freezing rates on the storage of ram semen
230. The role of antifreeze proteins in the freezing of ram spermatozoa
231. The resistance of semen to freezing in rams of several breeds (in Russian)
232. The suitability of some semen diluents for deep freezing ram semen in minitubes
233. Fertility of frozen and fresh extended ram semen
234. Preservation of bull semen
235. Freezing of ram semen in lactose-yolk-ethylene glycol diluent
236. The problem of freezing ram semen
237. Effect of freezing on the adenosine polyphosphate level of semen
238. The theoretical and practical basis of freezing the semen of farm animals
239. Study on freezing ram semen
240. Cryopreservation of ram spermatozoa
241. The protective action of albumin in the freezing of ram spermatozoa
242. The Role of Egg Yolk and of Other Factors in Deep-freezing of Bull and Ram Semen
243. Technique of freezing ram semen
244. Freezing ram semen in diluents containing polysaccharides
245. Aims and prospects for freezing semen
246. An experiment on the use of deep frozen ram semen
247. The technology of long term storage of semen
248. Storage of semen at −21°C
249. Revival of spermatozoa after vitrification and dehydration at low temperatures
250. Effect of cryoprotective diluent and method of freeze-thawing on survival and acrosomal integrity of ram spermatozoa
251. Recherches sur les animalcules spermatiques
252. Comparative studies on the activity of phospholipase A in the plasma membrane of spermatozoa during cryoconservation
253. Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and a new thawing procedure
254. The effect of cold shock and deep-freezing on ram spermatozoa collected by electrical ejaculation and by artificial vagina
255. Deep freezing of ram semen
256. The artificial insemination of sheep in Finland
257. Studies on the action of liquid nitrogen on cultures in vitro of fibroblasts
258. The effect of freeze-thawing of goat and sheep semen at a high density of spermatozoa on cell viability and fertility after insemination
259. Influence of freeze rate, thaw rate and glycerol level on acrosomal retention and survival of bovine spermatozoa frozen in French straws
260. Use of aloe vera gel in deep-freezing of ram spermatozoa for ovine artificial insemination
261. Preservation of ram semen using aloe vera derivatives as a diluent component
262. Cervical versus intrauterine insemination of ewes using fresh or frozen semen diluted with aloe vera gel
263. Glycérine et résistance du sperme aux basses températures
264. Pellet freezing of ram semen
265. Results of introduction into practice of frozen ram semen
266. Freezing ram semen in Cassou straws
267. Observations on fertility of ram semen frozen by different methods
268. Deep freezing of ram semen: recovery of spermatozoa after pelleting and comparison with other methods
269. The survival of ram spermatozoa following pellet freezing below −79°C
270. Fertility of ram spermatozoa following pellet freezing on dry ice at −79°C and −140°C
271. Fertility of ram spermatozoa frozen-stored for three years
272. Fertility of ram and boar semen after long-term storage
273. Fertility following deposition of equal numbers of frozen-thawed ram spermatozoa by single and double insemination
274. Fertility of ram semen after long-term storage
275. Effect of composition of the thawing solution on survival of ram spermatozoa frozen by the pellet method
276. Freezing of ram spermatozoa by the pellet method. I. The effect of diluent composition on survival of spermatozoa
277. Fertility of ram spermatozoa frozen by the pellet method. III. The effects of insemination technique, oxytocin and relaxin on lambing
278. Effect of composition of tris-based diluent and of thawing solution on survival of ram spermatozoa frozen by the pellet method
279. Fertility of ram spermatozoa frozen-stored for 5 years
280. Frozen storage of ram semen II. Causes of low fertility after cervical insemination and methods of improvement
281. Ein Beitrag zur Tiefkühlkonservierung von Schaf- und Ziegenbocksamen mit Hilfe des Pellet-bzw
282. Fertility of ram spermatozoa pellet-frozen in novel diluents
283. Proline and glycine betaine in cryoprotective diluents for ram spermatozoa
284. Preservation of ram spermatozoa. I. Effects on motility, livability and morphology of ram spermatozoa in four semen diluents
285. Longevity of fowl spermatozoa in frozen condition
286. Viability of spermatozoa of the chicken under various environmental conditions
287. Über den Einfluss niederer Temperaturgrade auf einige Elementarorganismen
288. The effect of dialysis of extended ram semen prior to freezing on post-thaw survival and fertility
289. The effect of nonpenetrating cryoprotectants added to TEST-yolk-glycerol extender on the post-thawing motility of ram spermatozoa
290. Versuche über die Gefrierkonservierung von Bullen-, Ziegenbock- und Schafbock-sperma in Pelletform
291. The use of egg yolk lipoproteins of low molecular weight in diluents for frozen semen of rams
292. Comparison of some semen diluents for use in deep freezing of ram semen
293. The optimum regime for freezing ram semen
294. Befruchtungsergebnisse nach Samenübertragung mit tiefgefrorenem Schafbock-Sperma (Minitüb) unter Verwendung verschiedener Verdünner
295. Artificial insemination of ewes with deep-frozen semen
296. Devices for thawing ram semen
297. Questionable protection by intracellular glycerol during freezing and thawing
298. Effect of glycerol on the penetrability of fresh ram spermatozoa with zona-free hamster eggs
299. Storage of semen of farm animals by deep-freezing
300. Deep freezing of semen of farm animals
301. Preservation of bull semen at a temperature of minus 78° centigrade
302. More importance to be given to the problem of dilution and storage of ram semen
303. Osmotic pressure of semen and its importance in freezing of spermatozoa
304. The effect of some physical and biological factors on freezing of ram semen
305. Storage of bull spermatozoa at low temperatures
306. AI of sheep with frozen semen
307. Evaluation of the fertility of frozen ram semen based on the state of the acrosomes
308. Frozen semen can fertilize and result in normal progeny
309. New method of thawing deep-frozen semen of animals
310. Opuscoli di Fiscia Animale, e Vegetabile
311. Collective thawing of ram semen pellets frozen on liquid nitrogen
312. Osmotic pressure and pH effects on survival of frozen bovine spermatozoa
313. An experiment on deep-cervical insemination of sheep with frozen semen
314. News on insemination of meat-wool producing sheep
315. Versuche zur Verlegung des Auftauvorganges von Tiefgefriersperma ins weibliche Genitale beim Rind
316. Lait écrémé en poudre pour dilution et la congélation du sperme du bélier
317. Insemination of sheep with frozen semen: effect of different diluents on motility, acrosome integrity and Sephadex-filtration of spermatozoa processed in mini-straws
318. Investigations on the freezing of ram semen using different diluents and in vitro evaluation procedures
319. Physiological and biochemical characteristics of semen should be considered in insemination with frozen semen
320. Biochemistry of ram semen and the development of an in vivo assay for sperm fertilising ability
321. Insemination of sheep with fresh or frozen semen
322. Congelación de semen de carnero en pastillas (pellets). 1. Efecto de la congelación sobre la motilidad progresiva, la morfología espermática y la fertilidad
323. Inseminación artificial en ovinos con semen congelado
324. Review of AI with frozen buck and ram semen in Greece
325. Introduction of new technology into the industry (in Russian)
326. The use of frozen semen
327. Possible cause of low fertilizing capacity of frozen semen
328. The effect of synthetic prostaglandins in frozen semen on conception rate of ewes (in Russian)
329. Some results of studies on freezing ram semen
330. Factors affecting the conception rate of ewes inseminated with frozen semen
331. Results of artificial insemination with frozen semen
332. Formulation of a protectant for deep freezing of ram semen
333. Improvement of the method of freezing semen
334. Deep freezing of ram semen
335. The effect of chelating agents and free radical scavangers on survival of bull spermatozoa at ambient temperature
336. Fertility of ram spermatozoa frozen in a tris-based diluent
337. Fertility following inseminations with frozen-thawed reconcentrated and unconcentrated ram semen
338. The effect of pellet volume, dilution rates prefreezing and at thawing and of thawing temperature on the survival and acrosome morphology of frozen ram spermatozoa
339. The effect of freezing method on the survival of ram spermatozoa
340. Freezing of ram semen by means of liquid nitrogen and results of its use compared with fresh semen
341. Conception in ewes inseminated with deep frozen semen
342. A criticism of the method of pellet freezing of semen
343. Technology of deep freezing of semen
344. Studies on the improvement of frozen ram semen
345. The interaction of egg yolk and ram spermatozoa studied with a fluorescent probe
346. Protection of ram and bull spermatozoa by the low density lipoprotein fraction of egg yolk during storage at 5°C and deep freezing
347. The preservation of semen in mammals
348. Influence of butylated hydroxytoluene, BHT on the viability of ram spermatozoa undergoing cold shock
349. A comparison of changes in the acrosomes of deep-frozen ram and bull spermatozoa
350. The response of ram spermatozoa to preparations of egg yolk in semen diluents during storage at 5 or − 196°C
351. Regions of the freezing curve causing changes in structure and viability of ram sperm
352. Effects of egg yolk, glycerol and the freezing rate on the viability and acrosomal structures of frozen ram spermatozoa
353. The influence of some fractions of egg yolk on the survival of ram spermatozoa at 5°C
354. Deep freezing of boar semen. II. Effects of method of dilution, glycerol concentration, and time of semen-glycerol contact on survival of spermatozoa
355. Procedures for freezing ram semen in straws
356. Diluents for freezing of ram semen
357. Results obtained in freezing ram semen
358. Results of artificial insemination in sheep using frozen semen (in Rumanian)
359. Damage to ram spermatozoa during the process of equilibration and freezing
360. Improvement of the methods for freezing ram semen
361. Freezing of ram semen in dextran containing diluents
362. The effect of the thawing regime on the quality and fertilizing ability of frozen ram semen
363. Fertilizing capacity of ram spermatozoa frozen in raffinose and lactose-gum arabic media
364. Effect of hypertonic media on spermatozoa