Extracellular production of human parathyroid hormone as a thioredoxin fusion form in Escherichia coli by chemical permeabilization combined with heat treatment

Biotechnology Progress

Volumn 21, Issue 5, 2005, Pages 1429-1435

  Fu, Xiang Yang ^a   Tong, Wang Yu ^a   Wei, Dong Zhi ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; ESCHERICHIA COLI; FERMENTATION; GENES; HEAT TREATMENT; OPTIMIZATION; PROTEINS;

CELL GROWTH; DENATURATION; PROTEIN RELEASE; VOLUMETRIC YIELD;

HORMONES;

HYBRID PROTEIN; PARATHYROID HORMONE; THIOREDOXIN;

ARTICLE; BIOREACTOR; BIOSYNTHESIS; CULTURE TECHNIQUE; ESCHERICHIA COLI; EXTRACELLULAR FLUID; FRACTIONATION; GENETICS; HEAT; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; PERMEABILITY; PROTEIN ENGINEERING;

BIOREACTORS; CELL CULTURE TECHNIQUES; CHEMICAL FRACTIONATION; ESCHERICHIA COLI; EXTRACELLULAR FLUID; HEAT; HUMANS; PARATHYROID HORMONE; PERMEABILITY; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; THIOREDOXIN;

ESCHERICHIA COLI;

EID: 26844559002     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp050137z     Document Type: Article

References (20)

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