Optimal conditions for the papain digestion of polyclonal ovine IgG for the production of biotherapeutic Fab fragments

Biotechnology and Applied Biochemistry

Volumn 42, Issue 2, 2005, Pages 163-168

  Cresswell, Chrissie ^a   Newcombe, Anthony R ^a   Davies, Susannah ^a   Macpherson, Ian ^b   Nelson, Paul ^b   O'Donovan, Kieran ^a   Francis, Richard ^a  

^a BIOCOMPATIBLES UK LTD   (United Kingdom)

^b PRISM Training and Consultancy Ltd   (United Kingdom)

Author keywords

Biotherapeutic; Fab fragment; Ovine IgG; Papain; Polyclonal; Proteolytic digestion

Indexed keywords

ANTIBODIES; BIOTECHNOLOGY; CELL CULTURE; DRUG PRODUCTS; MATHEMATICAL MODELS; PRECIPITATION (CHEMICAL); SODIUM COMPOUNDS; TIME AND MOTION STUDY;

BIOTHERAPEUTICS; FAB FRAGMENTS; OVINE IGG; PAPAIN; POLYCLONAL; PROTEOLYTIC DIGESTION;

PROTEINS;

IMMUNOGLOBULIN F(AB) FRAGMENT; PAPAIN; POLYCLONAL ANTIBODY; POLYCLONAL ANTIBODY OVINE IGG; SODIUM SULFATE; UNCLASSIFIED DRUG;

ABSORPTION; ARTICLE; IMMUNOGLOBULIN PRODUCTION; MATHEMATICAL MODEL; PH; PROTEIN ANALYSIS; PROTEIN PURIFICATION; TEMPERATURE SENSITIVITY; TIME;

ANIMALS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN G; KINETICS; PAPAIN; SHEEP; TEMPERATURE; TIME FACTORS;

MAMMALIA; OVIS;

EID: 26844558068     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20050020     Document Type: Article

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