A lead compound for the development of ABA 8′-hydroxylase inhibitors

Bioorganic and Medicinal Chemistry Letters

Volumn 15, Issue 23, 2005, Pages 5226-5229

  Ueno, Kotomi ^a   Yoneyama, Hidetaka ^b   Saito, Shigeki ^c   Mizutani, Masaharu ^c   Sakata, Kanzo ^c   Hirai, Nobuhiro ^c   Todoroki, Yasushi ^b  

^a GIFU UNIVERSITY   (Japan)

^b SHIZUOKA UNIVERSITY   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

ABA; ABA 8 hydroxylase; Abscisic acid; Cytochrome P450; Inhibitor

Indexed keywords

6 NOR 2',3' DIHYDRO 4' DEOXOABSCISIC ACID; ABSCISIC ACID; ABSCISIC ACID 8' HYDROXYLASE; ENZYME INHIBITOR; OXYGENASE; UNCLASSIFIED DRUG;

ARTICLE; COMPETITIVE INHIBITION; DRUG DESIGN; DRUG EFFICACY; DRUG HYDROXYLATION; DRUG MECHANISM; DRUG POTENCY; DRUG SPECIFICITY; DRUG STRUCTURE; DRUG SYNTHESIS; ENZYME SUBSTRATE; INHIBITION KINETICS;

ABSCISIC ACID; CYTOCHROME P-450 ENZYME SYSTEM; DRUG DESIGN; ENZYME INHIBITORS; MIXED FUNCTION OXYGENASES;

EID: 26844503964     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2005.08.042     Document Type: Article

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15. Lettuce seed germination assay. Twenty-five seeds of lettuce (Lactuca sativa L. cv. Grand Rapids) were placed on two sheets of Advantec No. 2 filter paper soaked in 2 mL of a test solution in a polystyrene dish (60 mm i.d.) and allowed to germinate under illumination (6000 lux) at 25°C. After 48 h, the inhibition ratio was defined as [(A - B)/A] × 100, where A is the number of seeds that germinated when water was used, and B is the number of seeds that germinated when a test compound was used. Rice seedling elongation assay. Seeds of rice (Oryza sativa L. cv. Nipponbare) were sterilized with EtOH for 5 min and washed with running tap water. The sterilized seeds were soaked in water to germinate for 3 days at 25°C. The seeds were then placed in a glass tube (40 mm i.d.) containing 2 mL of a test solution and grown with the tube sealed with a plastic cap under continuous light (6000 lux) at 25°C. When the seedlings were 7 days old, the length of the second leaf sheath was measured, and the inhibition ratio was calculated. The inhibition ratio is defined as [(A - B)/A] × 100, where A is the mean length of the second leaf sheath when water was used, and B is the mean length of the second leaf sheath when a test compound was used.
16. -1; detection, 254 nm.