Comparative analysis of the DNA staining efficiencies of different fluorescent dyes in preparative agarose gel electrophoresis

Clinical Chemistry and Laboratory Medicine

Volumn 43, Issue 8, 2005, Pages 841-842

  Huang, Qing ^a   Fu, Wei Ling ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Agarose gel electrophoresis; Ethidium bromide; Fluorescent dyes; GoldView ; Methods; SYBR Gold; SYBR Green

Indexed keywords

DNA; FLUORESCENT DYE;

AGAR GEL ELECTROPHORESIS; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DNA DETERMINATION; ELECTROPHORETIC MOBILITY; FLUORESCENCE ANALYSIS; PRIORITY JOURNAL; SAFETY; SENSITIVITY ANALYSIS;

COST-BENEFIT ANALYSIS; DNA; ELECTROPHORESIS, AGAR GEL; ETHIDIUM; FLUORESCENT DYES; ORGANIC CHEMICALS; SAFETY; STAINING AND LABELING;

EID: 26844450211     PISSN: 14346621     EISSN: 14346621     Source Type: Journal    
DOI: 10.1515/CCLM.2005.141     Document Type: Article

References (6)

1. Bensaude O. Ethidium bromide and safety - readers suggest alternative solutions. Trends Genet 1988;4:89-90.
2. Lunn G, Sansone EB. Ethidium bromide: destruction and decontamination of solutions. Anal Biochem 1987; 162:453-8.
3. Lunn G. Decontamination of ethidium bromide spills. Trends Genet 1990;6:31.
4. Singer VL, Lawlor TE, Yue S. Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test). Mutat Res 1999;439:37-47.
5. Tuma RS, Beaudet MP, Jin X, Jones LJ, Cheung CY, Yue S, et al. Characterization of SYBR Gold nucleic acid gel stain: a dye optimized for use with 300-nm ultraviolet transilluminators. Anal Biochem 1999;268:278-88.
6. Miller SE, Taillon-Miller P, Kwok PY. Cost-effective staining of DNA with SYBR green in preparative agarose gel electrophoresis. Biotechniques 1999;27:34-6.