A Bacillus thuringiensis isolation method utilizing a novel stain, low selection and high throughput produced atypical results

BMC Microbiology

Volumn 5, Issue , 2005, Pages

  Rampersad, Joanne ^a   Ammons, David ^a  

^a UNIVERSITY OF THE WEST INDIES   (Trinidad and Tobago)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACILLUS THURINGIENSIS; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BIOASSAY; CRYSTAL STRUCTURE; DNA LIBRARY; DNA SEQUENCE; ELECTRON MICROSCOPY; ENVIRONMENT; GENETIC SCREENING; HIGH THROUGHPUT SCREENING; HYBRIDIZATION; ISOLATION PROCEDURE; MICROSCOPY; MOLECULAR PROBE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; STAINING;

ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; BASE SEQUENCE; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION PROFILING; LARVA; SPODOPTERA;

BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS);

EID: 26844444250     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-5-52     Document Type: Article

References (30)

1. Bernhard K, Jarrett P, Meadows M, Butt J, Ellis DJ, Roberts GM, Pauli S, Rodgers P, Burges HG: Natural isolates of Bacillus thuringiensis: World wide Distribution, Characterization, and Activity against Insect Pests. J Invertebr Pathol 1997, 70:59-68.
2. Vilas-Boas GT, Lemos MVF: Diversity of cry genes and genetic characterization of Bacillus thuringiensis isolated from Brazil. Can J Microbiol 2004, 50:605-613.
3. Martin PAW, Travers RS: Worldwide abundance and distribution of Bacillus thuringiensis isolates. Appl Environ Microbiol 1989, 55:2437-2442.
4. Johnson C, Bishop AH: A technique for the effective enrichment and isolation of Bacillus thuringiensis. FEMS Microbiology Letters 1996, 142:173-177.
5. Chak KF, Chao DC, Tseng MY, Kao SS, Tuan SJ, Feng TY: Determination and Distribution of cry-Type genes of Bacillus thuringiensis isolates from Taiwan. Appl Environ Microbiol 1994, 60:2415-2420.
6. Ben Dov E, Zaritsky A, Dahan E, Barak Z, Sinai R, Manasherob R, Khamrev A, Troitskaya E, Dubitsky A, Berezina N, Margalith Y: Extended Screening by PCR for seven cry group genes from field-collected strains of Bacillus thuringiensis. Appl Environ Microbiol 1997, 63:4883-4890.
7. Rampersad J, Khan A, Ammons D: Usefulness of Staining Parasporal Bodies when Screening for Bacillus thuringiensis. J Invertebr Pathol 2002, 79:203-4.
8. Rampersad J, Khan A, Ammons D: A Bacillus thuringiensis isolate possessing a spore-associated filament. Curr Microbiol 2003, 47:355-357.
9. Ohba M, Aizawa K: Serological identification of Bacillus thuringiensis and Related Bacteria isolated in Japan. J Invertebr Pathol 1978, 32:303-309.
10. Hastowo S, Lay BW, Ohba M: Naturally occurring Bacillus thuringiensis in Indonesia. J Appl Bacterio 1992, 73:108-113.
11. Aronson A: Sporulation and delta-endotoxin synthesis in Bacillus thuringiensis. Cell Mol Life Sci 2002, 59:417-425.
12. Hodgkiss W: Filamentous appendages on the spores and exosporium of certain Bacillus species. In Spore Research Edited by: Barker AN, Gould GW, Wolf J. Academic Press, London and New York; 1971:211-218.
13. Mizuki E, Ohba M, Ichimatsu T, Hwang SH, Higuchi K, Saitoh H, Akao T: Unique apppendages associated with spores of Bacillus cereus isolates. J Basic Microbiol 1998, 38:33-39.
14. Wei JZ, Hale K, Carta L, Platzer E, Wong C, Fang SC, Aroian RV: Bacillus thuringiensis crystal proteins that target nematodes. Proc Natl Acad Sci USA 2003, 100:2760-5.
15. Bravo A, Sarabia S, Lopez L, Ontiveros H, Abarca C, Ortiz A, Ortiz M, Lina L, Villalobos FJ, Pena G, Nunez-Valdez M, Soberon M, Quintero R: Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection. Appl Environ Microbiol 1998, 64:4965-4972.
16. Ohba M, Aizawa K: Distribution of Bacillus thuringiensis in Soils of Japan. J Invertebr Pathol 1986, 47:277-282.
17. Bel Y, Granero F, Alberola TM, Martinez-Sebastian MJ, Ferre J: Distribution, frequency and diversity of Bacillus thuringiensis in olive tree environments in Spain. System Appl Microbiol 1997, 20:652-658.
18. Meadows MP, Ellis DJ, Butt J, Jarrett P, Burges HD: Distribution, Frequency and diversity of Bacillus thuringiensis in an Animal Feed Mill. Appl Environ Microbiol 1992, 58:1344-1350.
19. Stewart GSAB, Johnstone K, Hagelberg E, Ellar DJ: Commitment of bacterial spores to germinate. Biochem J 1981, 198:101-106.
20. DeLucca AJ, Simonson JG, Larson AD: Bacillus thuringiensis distribution in soils of the United States. Can J Microbiol 1981, 27:865-870.
21. Smith RA, Couche GA: The phylloplane as a source of Bacillus thuringiensis variants. Appl Environ Microbiol 1991, 57:311-315.
22. Kaelin P, Morel P, Gadani F: Isolation of Bacillus thuringiensis from stored tobacco and Lasioderma serricorne (F). Appl Environ Microbiol 1994, 60:19-25.
23. Lamelli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
24. Sambrook J, Fitsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual Cold Spring Harbor, Cold Spring Harbor Press; 1989.
25. Dyar HG: The number of moults of lepidopterous larvae. Psyche 1890, 5:420-422.
26. Travers RS, Martin PAW, Reichelderfer CF: Selective Process for Efficient Isolation of Soil Bacillus spp. Appl Environ Microbiol 1987, 56:1263-1266.
27. Juarez-Perez VM, Ferrandis MD, Frutos R: PCR Based Approach for the Detection of novel Bacillus thuringiensis cry genes. Appl Environ Microbiol 1997, 63:2977-3002.
28. Masson L, Erlandson M, Puzstai-Carey M, Brousseau R, Juarez-Perez V, Frutos R: A Holistic Approach for determining the Entomopathogenic potential of Bacillus thuringiensis strains. Appl Environ Microbiol 1998, 64:4782-4788.
29. Ceron J, Ortiz A, Quintero R, Guereca L, Bravo A: Specific PCR primers directed to identify cry I and cry III genes within a Bacillus thuringiensis strain collection. Appl Environ Microbiol 1995, 61:3826-3831.
30. Siefert JL, Larios-Sanz M, Nakamura LK, Slepecky RA, Paul JH, Moore ERB, Fox GE, Jurtshuk P Jr: Phylogeny of Marine Bacillus Isolates from the Gulf of Mexico. Curr Microbiol 2000, 41:84-88.