Use of random and saturation mutageneses to improve the properties of Thermus aquaticus amylomaltase for efficient production of cycloamyloses

Applied and Environmental Microbiology

Volumn 71, Issue 10, 2005, Pages 5823-5827

  Fujii, Kazutoshi ^a   Minagawa, Hirotaka ^b   Terada, Yoshinobu ^a   Takaha, Takeshi ^a   Kuriki, Takashi ^a   Shimada, Jiro ^b   Kaneko, Hiroki ^c  

^a EZAKI GLICO CO LTD   (Japan)

^b NEC CORPORATION   (Japan)

^c NIHON UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CATALYSIS; HYDROLYSIS; MICROBIOLOGY; MUTAGENESIS; POLYMERIZATION;

AMYLOMALTASE; CYCLIZATION ACTIVITY; HYDROLYTIC ACTIVITY; INTRAMOLECULAR TRANSGLYCOSYLATION;

ENZYMES;

4ALPHA GLUCANOTRANSFERASE; CYCLODEXTRIN;

ENZYME;

ARTICLE; CHEMICAL REACTION; COMPLEX FORMATION; CYCLIZATION; ENZYME ACTIVITY; ENZYME STRUCTURE; GENE ORDER; GLYCOSYLATION; MOLECULAR INTERACTION; MUTAGENESIS; NONHUMAN; POLYMERIZATION; SITE DIRECTED MUTAGENESIS; THERMUS AQUATICUS; WILD TYPE;

AMINO ACID SUBSTITUTION; CYCLODEXTRINS; GLYCOGEN DEBRANCHING ENZYME SYSTEM; INDUSTRIAL MICROBIOLOGY; MUTAGENESIS; PROTEIN ENGINEERING; THERMUS;

THERMUS AQUATICUS;

EID: 26844434953     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.10.5823-5827.2005     Document Type: Article

References (26)

1. Endo, T., M. Zheng, and W. Zimmermann. 2002. Enzymatic synthesis and analysis of large-ring cyclodextrins. Aust. J. Chem. 55:39-48.
2. Frömming, K.-H., and J. Szejtli. 1994. Cyclodextrins in pharmacy. Kluwer Academic Publishers, Dordrecht, The Netherlands.
3. Fujii, K., H. Takata, M. Yanase, Y. Terada, K. Ohdan, T. Takaha, S. Okada, and K. Kuriki. 2003. Bioengineering and application of novel glucose polymer. Biocatal. Biotransform. 21:167-172.
4. Gessler, K., I. Uson, T. Takaha, N. Krauss, S. M. Smith, S. Okada, G. M. Sheldrick, and W. Saenger. 1999. V-amylose at atomic resolution: X-ray structure of a cycloamylose with 26 glucoses. Proc. Natl. Acad. Sci. USA 96:4246-4251.
5. Hedges, A. R. 1998. Industrial applications of cyclodextrins. Chem. Rev. 98:2035-2044.
6. Imamura, K., T. Matsuura, M. Ye, T. Takaha, K. Fujii, M. Kusunoki, and Y. Nitta. 2005. Crystallization and preliminary X-ray crystallographic study of disproportionating enzyme from potato. Acta Crystallogr. 61:109-111.
7. Kitamura, S., K. Nakatani, T. Takaha, and S. Okada. 1999. Complex formation of large-ring cyclodextrins with iodine in aqueous solution as revealed by isothermal titration calorimetry. Macromol. Rapid Commun. 20:612-615.
8. Leemhuis, H., B. W. Dijkstra, and L. Dijkhuizen. 2002. Mutations converting cyclodextrin glycosyltransferase from a transglycosylase into a starch hydrolase. FEES Lett. 514:189-192.
9. Machida, S., S. Ogawa, S. Xiaohua, T. Takaha, K. Fujii, and K. Hayashi. 2000. Cycloamylose as an efficient artificial chaperone for protein refolding. FEBS Lett. 486:131-135.
10. Miwa, I., J. Okuda, K. Maeda, and G. Okuda. 1972. Mutarotase effect on colorimetric determination of blood glucose with β-D-glucose oxidase. Clin. Chim. Acta 37:538-540.
11. Nakamura, A., K. Haga, and K. Yamane. 1994. Four aromatic resides in the active center of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011: effects of replacements on substrate binding and cyclization characteristics. Biochemistry 33:9929-9936.
12. Penninga, D., B. Strokopytov, H. J. Rozeboom, C. L. Lawson, B. W. Dijkstra, J. Bergsma, and L. Dijkhuizen. 1995. Site-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulons strain 251 affect activity and product specificity. Biochemistry 34:3368-3376.
13. Przylas, I., K. Tomoo, Y. Terada, T. Takaha, K. Fujii, W. Saenger, and N. Sträter. 2000. Crystal structure of amylomaltase from Thermus aquaticus, a glycosyltransferase catalyzing the production of large cyclic glucans. J. Mol. Biol. 296:873-886.
14. Przylas, I., Y. Terada, K. Fujii, T. Takaha, W. Saenger, and N. Sträter. 2000. 2000. X-ray structure of acarbose bound to amylomaltase from Thermus aquaticus. Eur. J. Biochem. 267:6903-6913.
15. Szejtli, J. 1998. Cyclodextrin technology, Kluwer Academic Publishers, Dordrecht, The Netherlands.
16. Szejtli, J. 1998. Introduction and general overview of cyclodextrin chemistry. Chem. Rev. 98:1743-1753.
17. Takaha, T., and S. M. Smith. 1999. The function of 4-α- glucanotransferases and their use for the production of cyclic glucans. Biotechnol. Genet. Eng. Rev. 16:257-280.
18. Takaha, T., M. Yanase, H. Takata, S. Okada, and S. M. Smith. 1996. Potato D-enzyme catalyzes the cyclization reaction of amylose to produce cycloamylose, a novel cyclic glucan. J. Biol. Chem. 271:2902-2908.
19. Takeda, Y., H.-P. Guan, and J. Preiss. 1993. Branching of amylose by the branching isoenzymes of maize endosperm. Carbohydr. Res. 240:1600-1606.
20. Terada, Y., K. Fujii, T. Takaha, and S. Okada. 1999. Thermus aquaticus ATCC 33923 amylomaltase gene cloning and expression and enzyme characterization: production of cycloamylose. Appl. Environ. Microbiol. 65:910-915.
21. Terada, Y., M. Yanase, H. Takata, T. Takaha, and S. Okada. 1997. Cyclodextrins are not the major cyclic a-1,4-glucans produced by the initial action of cyclodextrin glucanotransferase on amylose. J. Biol. Chem. 272:15729-15733.
22. Uitdehaag, J. C. M., B. A., van der Veen, L. Dijkhuizen, and B. W. Dykstra. 2002. Catalytic mechanism and product specificity of cyclodextrin glycosyl-transferase, a prototypical transglycosylase from the α-amylase family. Enzyme Microb. Technol. 30:295-304.
23. van der Veen, B. A., J. C. M. Uitdehaag, B. W. Dijkstra, and L. Dijkhuizen. 2000. The role of arginine 47 in the cyclization and coupling reactions of cyclodextrin glycosyltransferase from Bacillus circulons strain 251: implications for product inhibition and product specificity. Eur. J. Biochem. 267:3432-3441.
24. van der Veen, B. A., J. C. M. Uitdehaag, B. W. Dijkstra, and L. Dijkhuizen. 2000. Engineering of cyclodextrin glycosyltransferase reaction and product specificity. Biochim. Biophys. Acta 1543:336-360.
25. van der Veen, B. A., J. C. M. Uitdehaag, D. Penninga, G. J. van Alebeek, L. M. Smit, B. W. Dijkstra, and L. Dijkhuizen. 2000. Rational design of cyclodextrin glycosyltransferase from Bacillus circulons strain 251 to increase alpha-cyclodextrin production. J. Mol. Biol. 296:1027-1038.
26. Yanase, M., H. Takata, T. Takaha, T. Kuriki, S. M. Smith, and S. Okada. 2002. Cyclization reaction catalyzed by glycogen debranching enzyme (EC 2.4.1.25/EC 3.2.1.33) and its potential for cycloamylose production. Appl. Environ. Microbiol. 68:4233-4239.