Phospho-eNOS Ser-114 in human mesenchymal stem cells: Constitutive phosphorylation, nuclear localization and upregulation during mitosis

European Journal of Cell Biology

Volumn 84, Issue 10, 2005, Pages 809-818

  Klinz, Franz Josef ^a   Schmidt, Annette ^b   Schinköthe, Timo ^a   Arnhold, Stefan ^a   Desai, Biren ^a   Popken, Frank ^a   Brixius, Klara ^a   Schwinger, Robert ^a   Mehlhorn, Uwe ^a   Staib, Peter ^a   Addicks, Klaus ^a   Bloch, Wilhelm ^b  

^a UNIVERSITY OF COLOGNE   (Germany)

^b GERMAN SPORT UNIVERSITY COLOGNE   (Germany)

Author keywords

8 Hydroxyguanosine; Bone marrow; Caveolin 1; Cell division; Nitric oxide synthase; Nucleus; Stromal cells; Superoxide anion

Indexed keywords

8 HYDROXYDEOXYGUANOSINE; CAVEOLIN 1; ENDOTHELIAL NITRIC OXIDE SYNTHASE; SERINE; SUPEROXIDE;

ARTICLE; BONE MARROW; CELL COMPARTMENTALIZATION; CELL NUCLEUS; CELL PROLIFERATION; CELLULAR DISTRIBUTION; CYTOSOL; ENZYME ACTIVITY; ENZYME LOCALIZATION; ENZYME PHOSPHORYLATION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IMMUNOREACTIVITY; MESENCHYMAL STEM CELL; MITOSIS; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; UPREGULATION;

EID: 26644445689     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejcb.2005.06.003     Document Type: Article

References (31)

1. L. Airas J. Hellman M. Salmi P. Bono T. Puurunen D.J. Smith S. Jalkanen CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73 J. Exp. Med. 182 1995 1603-1608
2. W.K. Alderton C.E. Coope R.G. Knowles Nitric oxide synthases: Structure, function and inhibition Biochem. J. 357 2001 593-615
3. F.P. Barry R.E. Boynton S. Haynesworth J.M. Murphy J. Zaia The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105) Biochem. Biophys. Res. Commun. 265 1999 134-139
4. P.M. Bauer D. Fulton Y.C. Boo G.P. Sorescu B.E. Kemp H. Jo W.C. Sessa Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase J. Biol. Chem. 278 2003 14841-14849
5. W. Bloch U. Mehlhorn A. Krahwinkel M. Reiner M. Dittrich A. Schmidt K. Addicks Ischemia increases detectable endothelial nitric oxide synthase in rat and human myocardium Nitric Oxide 5 2001 317-333
6. T. Bohle M.C. Brandt N. Henn A. Schmidt W. Bloch D.J. Beuckelmann Identification of the cardiac ryanodine receptor channel in membrane blebs of sarcoplasmic reticulum FEBS Lett. 505 2001 419-425
7. V.I. Bruskov A.I. Gaziev L.V. Malakhova I.A. Mantsygin O.S. Morenkov Monoclonal antibodies to 8-oxo-2′-deoxyguanosine (8-hydroxyguanosine). Characteristics and use for determining DNA damage by active forms of oxygen Biokhimiia 61 1996 737-744
8. E. Clarke S.R. McCann Age dependent in vitro stromal growth Bone Marrow Transplant. 4 1989 596-597
9. D.C. Colter R. Class C.M. DiGirolamo D.J. Prockop Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow Proc. Natl. Acad. Sci. USA 97 2000 3213-3218
10. P. Degan M.K. Shigenaga E.M. Park P.E. Alperin B.N. Ames Immunoaffinity isolation of urinary 8-hydroxy-2′-deoxyguanosine and 8-hydroxyguanine and quantitation of 8-hydroxy-2′-deoxyguanosine in DNA by polyclonal antibodies Carcinogenesis 12 1991 865-871
11. F. Deryckere F. Gannon A one-hour minipreparation technique for extraction of DNA-binding proteins from animal tissues Biotechniques 16 1994 405
12. S. Dimmeler E. Dernbach A.M. Zeiher Phosphorylation of the endothelial nitric oxide synthase at Ser-1177 is required for VEGF-induced endothelial cell migration FEBS Lett. 477 2000 258-262
13. L. Elzaouk S. Laufs D. Heerklotz W. Leimbacher N. Blau A. Resibois B. Thony Nuclear localization of tetrahydrobiopterin biosynthetic enzymes Biochim. Biophys. Acta 1670 2004 56-68
14. Y. Feng V.J. Venema R.C. Venema N. Tsai R.B. Caldwell VEGF induces nuclear translocation of Flk-1/KDR, endothelial nitric oxide synthase, and caveolin-1 in vascular endothelial cells Biochem. Biophys. Res. Commun. 256 1999 192-197
15. I. Fleming R. Busse Molecular mechanisms involved in the regulation of the endothelial nitric oxide synthase Am. J. Physiol. Regul. Integr. Comp. Physiol. 284 2003 R1-R12
16. A.J. Friedenstein J.F. Gorskaja N.N. Kulagina Fibroblast precursors in normal and irradiated mouse hematopoietic organs Exp. Hematol. 4 1976 267-274
17. D. Fulton J.P. Gratton W.C. Sessa Post-translational control of endothelial nitric oxide synthase: Why isn't calcium/calmodulin enough? J. Pharmacol. Exp. Ther. 299 2001 818-824
18. D. Fulton J. Fontana G. Sowa J.P. Gratton M. Lin K.X. Li B. Michell B.E. Kemp D. Rodman W.C. Sessa Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme J. Biol. Chem. 277 2002 4277-4284
19. J.Z. Gasiorowski D.A. Dean Mechanisms of nuclear transport and interventions Adv. Drug Deliv. Rev. 55 2003 703-716
20. M. Gilchrist S.D. McCauley A.D. Befus Expression, localization, and regulation of NOS in human mast cell lines: Effects on leukotriene production Blood 104 2004 462-469
21. A. Giordano C. Tonello A. Bulbarelli V. Cozzi S. Cinti M.O. Carruba E. Nisoli Evidence for a functional nitric oxide synthase system in brown adipocyte nucleus FEBS Lett. 514 2002 135-140
22. A. Gougos M. Letarte Identification of a human endothelial cell antigen with monoclonal antibody 44G4 produced against a pre-B leukemic cell line J. Immunol. 141 1988 1925-1933
23. M.H. Helfrich D.E. Evans P.S. Grabowski J.S. Pollock H. Ohshima S.H. Ralston Expression of nitric oxide synthase isoforms in bone and bone cell cultures J. Bone Miner. Res. 12 1997 1108-1115
24. R. Kou D. Greif T. Michel Dephosphorylation of endothelial nitric-oxide synthase by vascular endothelial growth factor. Implications for the vascular responses to cyclosporin A J. Biol. Chem. 277 2002 29669-29673
25. A.K. Majors C.A. Boehm H. Nitto R.J. Midura G.F. Muschler Characterization of human bone marrow stromal cells with respect to osteoblastic differentiation J. Orthop. Res. 15 1997 546-557
26. J.B. Michel O. Feron D. Sacks T. Michel Reciprocal regulation of endothelial nitric-oxide synthase by Ca2+-calmodulin and caveolin J. Biol. Chem. 272 1997 15583-15586
27. L. Osborn C. Hession R. Tizard C. Vassallo S. Luhowskyj G. Chi-Rosso R. Lobb Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes Cell 59 1989 1203-1211
28. M.F. Pittenger A.M. Mackay S.C. Beck R.K. Jaiswal R. Douglas J.D. Mosca M.A. Moorman D.W. Simonetti S. Craig D.R. Marshak Multilineage potential of adult human mesenchymal stem cells Science 284 1999 143-147
29. M. Reiner W. Bloch K. Addicks Functional interaction of caveolin-1 and eNOS in myocardial capillary endothelium revealed by immunoelectron microscopy J. Histochem. Cytochem. 49 2001 1605-1610
30. R.H. Schwinger M. Bohm U. Schmidt P. Karczewski U. Bavendiek M. Flesch E.G. Krause E. Erdmann Unchanged protein levels of SERCA II and phospholamban but reduced Ca2+ uptake and Ca2+ -ATPase activity of cardiac sarcoplasmic reticulum from dilated cardiomyopathy patients compared with patients with nonfailing hearts Circulation 92 1995 3220-3228
31. R.S. Tuan G. Boland R. Tuli Adult mesenchymal stem cells and cell-based tissue engineering Arthritis Res. Ther. 5 2003 32-45