N-Glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation

Protein Engineering, Design and Selection

Volumn 18, Issue 10, 2005, Pages 497-501

  Kwan, Emily M ^a,b   Boraston, Alisdair B ^a,b,c   McLean, Bradley W ^a,b,d   Kilburn, Douglas G ^a,b   Warren, R Antony J ^a,b  

^a Networks of Centres of Excellence   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c UNIVERSITY OF VICTORIA   (Canada)

^d Twinstrand Therapeutics   (Canada)

Author keywords

Carbohydrate binding module; Fusion proteins; N glycosidase

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; ENZYME IMMOBILIZATION; ENZYMES;

CARBOHYDRATE-BINDING MOLECULE; FUSION PROTEINS; GLYCOPROTEINS; N-GLYCOSIDASE;

PROTEINS;

BLOOD CLOTTING FACTOR 10A; CARBOHYDRATE BINDING PROTEIN; CELLULOSE; GLYCOSIDASE; GLYCOSIDE; HISTIDINE; HYBRID PROTEIN; N ACETYL BETA GLUCOSAMINIDASE; THROMBIN;

ARTICLE; DENATURATION; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ESCHERICHIA COLI; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION;

CHROMATOGRAPHY, AFFINITY; CHRYSEOBACTERIUM; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; FACTOR X; GLYCOPROTEINS; GLYCOSYLATION; HISTIDINE; MANNOSYL-GLYCOPROTEIN ENDO-BETA-N-ACETYLGLUCOSAMINIDASE; OLIGOPEPTIDES; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; POLYSACCHARIDES; RECOMBINANT FUSION PROTEINS; RIBONUCLEASES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

CHRYSEOBACTERIUM MENINGOSEPTICUM; ESCHERICHIA COLI;

EID: 26444508894     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzi055     Document Type: Article

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