Transbilayer complementarity of phospholipids. Proof of principle

Langmuir

Volumn 21, Issue 20, 2005, Pages 8983-8986

  Zhang, Jianbing ^a   Jing, Bingwen ^a   Regen, Steven L ^a  

^a LEHIGH UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUID PHASE; NEAREST-NEIGHBOR RECOGNITION (NNR); TRANSBILAYERS;

DISTANCE MEASUREMENT; FLUIDS; MEMBRANES; MONOLAYERS; PATTERN RECOGNITION;

PHOSPHOLIPIDS;

MYRISTIC ACID DERIVATIVE; PHOSPHOLIPID; STEARIC ACID DERIVATIVE;

ARTICLE; CELL MEMBRANE; CHEMICAL MODEL; CHEMICAL PHENOMENA; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; LIPID BILAYER; MEMBRANE FLUIDITY; METABOLISM; PHYSIOLOGY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; HYDROPHOBICITY; LIPID BILAYERS; MEMBRANE FLUIDITY; MEMBRANE MICRODOMAINS; MODELS, CHEMICAL; MYRISTATES; PHOSPHOLIPIDS; STEARIC ACIDS;

EID: 26444476571     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la052031j     Document Type: Article

References (17)

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9. 9-11 In principle, in the liquid-crystalline state, one can also imagine a situation in which the longer chain folds into the space below the shorter chain. For example, a phospholipid bearing one 18-carbon chain and one 12-carbon chain would resemble a phospholipid having two 15-carbon chains by this "wrap-around" model. It should be noted, however, that such a lipid (i.e., C), when included in a bilayer containing phospholipids with two 18-carbon chains and phospholipids with two 12-carbon chains, would still favor another C as a nearest-neighbor across the bilayer on the basis of the principle of transbilayer complementarity.
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16. The main argument that was used in our previous work was that if nucleation effects were negligible then both A′A′ and B′B′ should generate the same degree of stabilization and, hence, identical K < 4 values. If nucleation effects were important, however, then the longer homodimer template would be expected to yield a lower value of K. This argument does not rule out the possibility that differences in the effectiveness of A′A′ and B′B′ may be nondetectable.
17. Although it is tempting to try to correlate equilibrium concentrations with the strength of the communication between the two monolayer leaflets (i.e., the perfectness of transbilayer complementarity), this is not possible. For example, a bilayer that contains 20 mol % C′C′ does not require an equilibrium concentration for CC equaling 20 mol % for perfect complementarity. In particular, some fraction of C′C′ is expected to complement itself in the adjoining monolayer. In addition, when two exchangeable C units "sit across" from one C′C′, they may be connected to one another through a disulfide bond, or they may be connected to other neighboring lipids in the same monolayer. Both situations would reduce the quantity of CC dimer below 20 mol %. The question of the strength of complementarity is an interesting one, and we are currently trying to devise experiments that would allow us to measure it.