BCECF in single cultured cells: Inhomogeneous distribution but homogeneous response

Journal of Experimental Biology

Volumn 201, Issue 1, 1998, Pages 57-62

  Weinlich, Michael ^a,b   Theiß, Christiane ^a   Lin, Chiann Tso ^a   Kinne, Rolf K H ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

Author keywords

(2',7' bis 2 carboxyethyl) 5 (and 6)carboxyfluorcscein; Cellular compartments; Confocal laser scanning microscopy; Cytoplasmic pH; Hydrogen ion concentration; Mitochondria; Nucleus

Indexed keywords

2',7' BIS(CARBOXYETHYL) 5(6) CARBOXYFLUORESCEIN; 2',7'-BIS(CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN; AMMONIUM CHLORIDE; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; NIGERICIN;

ANIMAL; ARTICLE; CELL LINE; CELL NUCLEUS; CHEMISTRY; CONFOCAL MICROSCOPY; CYTOPLASM; HENLE LOOP; LASER; METABOLISM; MITOCHONDRION; PH; RABBIT; STAINING;

AMMONIUM CHLORIDE; ANIMALS; CELL LINE, TRANSFORMED; CELL NUCLEUS; CYTOPLASM; FLUORESCEINS; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; LASERS; LOOP OF HENLE; MICROSCOPY, CONFOCAL; MITOCHONDRIA; NIGERICIN; RABBITS; STAINING AND LABELING;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 2642708355     PISSN: 00220949     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. ALLEN, C. N., HARPUR, E. S., GRAY, T. J. B., SIMMONS, N. L. AND HIRST, B. H. (1990). Efflux of bis-carboxyethyl-carboxyfluorescein (BCECF) by a novel ATP-dependent transport mechanism in epithelial cells. Biochem. biophys. Res. Commun. 172, 262-267.
2. BRIGHT, G. R., WHITAKER, J. E., HAUGLAND, R. P. AND TAYLOR, D. L. (1989). Heterogeneity of the changes in cytoplasmic pH upon serum stimulation of quiescent fibroblasts. J. cell. Physiol. 141, 410-419.
3. 4 cells from intracellular alkalinization. Biochim. biophys. Acta 931, 326-334.
4. JOHNSON, L. V., WALSH, M. L. AND CHEN, L. B. (1980). Localisation of mitochondria in living cells with rhodamine 123. Proc. natn. Acad. Sci. U.S.A. 77, 990-994.
5. LIPSKY, N. G. AND PAGANO, R. E. (1985). A vital stain for the Golgi apparatus. Science 228, 745-747.
6. 2+ in monolayers and suspensions of various types of animal cells. J. Cell Biol. 105, 2145-2155.
7. MOLENAAR, D., BOLHUIS, H., ABEE, T., POOLMAN, B. AND KONINGS, W. N. (1992). The efflux of fluorescent probe is catalysed by an ATP-driven extrusion system in Lactococcus lactis. J. Bacteriol. 174, 3118-3124.
8. OPITZ, N., WEINLICH, M., MOOREN, F., KEINEMANN, F. K., WEIDEMANN, G. AND KINNE, R. K. H. (1991). Dual wavelength excitation in con focal laser scanning microscopy: Intracellular pH measurements with a fluorescent indicator. Biomed. Technik. 36, 132-133.
9. OWEN, C. S. (1992). Comparison of spectrum-shifting intracellular pH probes 5′(and 6′)-carboxy-10-dimethylamino-3-hydroxyspiro[7H-benco|c]xanthene-7, 1′(3′H)-isobenzofuran]-3′-one and 2′,7′-biscarboxyethyl-5(and 6)-carboxyfluorescein. Analyt. Biochem. 204, 65-71.
10. -) exchange in gastric glands. Am. J. Physiol. 250, G524-G534.
11. ROOS, A. AND BORON, W. F. (1981). Intracellular pH. Physiol. Rev. 61, 296-434.
12. + exchanger is basolateral. Am. J. Physiol. 260, C868-C876.
13. SCHOOLWERTH, A. C., GESEK, F. A. AND CULPEPPER, R. M. (1989). Proton compartmentation in rat renal cortical tubules. Am. J. Physiol. 256, F986-F993.
14. SCOTT, D. M., MACDONALD, C., BRZESKI, H. AND KINNE, R. (1986). Maintenance of expression of differentiation function of kidney cells following transformation of SV40 early region DNA. Exp. Cell Res. 166, 391-398.
15. SLAYMAN, C. L. AND MOUSSATOS, V. V. (1994). Endosomal accumulation of pH indicator dyes delivered as acetoxymethyl esters. J. exp. Biol. 196, 419-438.
16. STEINBERG, S. F., BILEZIKIAN, J. P. AND AL.-AWQATI, Q. (1987). Fura-2 fluorescence is localised to mitochondria in endothelial cells. Am. J. Physiol. 253, C744-C747.
17. TERASAKI, M., SONG, J., WONG, J. R., WEISS, M. J. AND CHEN, L. B. (1984). Localisation of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes. Cell 38, 101-108.
18. THOMAS, J. A., KOLBECK, P. C. AND LANGWORTHY, T. A. (1982). Spectrophotometric determination of cytoplasmic and mitochondrial pH transitions using trapped pH indicators. In Intracellular pH: Its Measurement, Regulation and Utilisation in Cellular Functions (ed. R. Nucitelli and D. W. Deamer), pp. 105-123. New York: Alan R. Liss.
19. TSIEN, R. Y. (1989). Fluorescent indicators of ion concentrations. Meth. Cell Biol. 30, 127-156.
20. 31P-NMR. Biochim. biophys. Acta 1136, 129-135.
21. 2+ gradients with subcellular organelles. J. cell. Physiol. 150, 593-609.
22. +/base transport in principal cells characterised by confocal fluorescence imaging. Am. J. Physiol. 259, C365-C373.
23. WEINLICH, M., CAPASSO, G. AND KINNE, R. K. H. (1993). Intracellular pH in renal tubules in situ: Single cell measurements by confocal laser scanning microscopy. Pflügers Arch. 422, 523-529.
24. WEINLICH, M., CAPASSO, G. AND KINNE, R. K. H. (1994). Confocal microscopy. In Methods in Membrane and Transporter Research (ed. J. A. Schafer, G. Giebisch, P. Kristensen and H. H. Ussing). pp. 215-272. Austin: R. G. Landes.