Screening for positive transfected clones with coexpressed green fluorescent protein

BioTechniques

Volumn 24, Issue 1, 1998, Pages 52-55

  Ngo, N ^a   Hou, C ^a   Cunningham, S ^a   Zhang, R B ^a   Li, Z ^a   Li, Xianqiang ^a   Huang, Chiao Chain ^a  

^a CLONTECH Laboratories Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA POLYMERASE; GREEN FLUORESCENT PROTEIN; LUCIFERASE; TETRACYCLINE;

ARTICLE; CLONE; DRUG RESISTANCE; FLUORESCENCE MICROSCOPY; GENE; GENE EXPRESSION; IMMUNOBLOTTING; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCREENING;

EID: 2642689684     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98241bm10     Document Type: Article

References (7)

1. Baron, U., S. Freundlich, M. Gossen and H. Bujard. 1995. Co-regulation of two gene activities by tetracycline via a bidirectional promoter. Nucleic Acids Res. 23:3605-3606.
2. Cormack, B.P., R.H. Valdivia and S. Falkow. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38.
3. Gossen, M. and B. Bujard. 1992. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89:5547-5551.
4. Jurman, M.E., L.M. Boland, Y. Liu and G. Yellen. 1994. Visual identification of individual transfected cells for electrophysiology using antibody-coated beads. BioTechniques 17:876-881.
5. Mosser, D.D., A.W. Caron, L. Bourget, P. Jolicoeur and B. Massie. 1997. Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products. BioTechniques 22:150-161.
6. Schenborn, E. and V. Goiffon. 1993. Promega notes 41:11.
7. Yang, T.T., L. Cheng and S.T. Kain. 1996. Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein. Nucleic Acids Res. 24:4592-4593.