pMECA: A cloning plasmid with 44 unique restriction sites that allows selection of recombinants based on colony size

BioTechniques

Volumn 24, Issue 6, 1998, Pages 922-928

  Michael Thomson, J ^a   Parrott, Wayne A ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID VECTOR;

BACTERIAL GENE; BACTERIUM COLONY; GENE MAPPING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; SHORT SURVEY;

BACTERIA (MICROORGANISMS);

EID: 2642647827     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98246bm04     Document Type: Short Survey

References (7)

1. Alexeyev, M.F. 1995. Three kanamycin resistance gene cassettes with different polylinkers. BioTechniques 18:52-56.
2. Chen, G., I. Choi, B. Ramachandran and J.E. Gouaux. 1994. Total gene synthesis: novel single-step and convergent strategies applied to the construction of a 779 base pair bacteriorhodopsin gene. J. Am. Chem. Soc. 116:8799-8800.
3. DilIon, P.J. and C.A. Rosen. 1990. A rapid method for the construction of synthetic genes using the polymerase chain reaction. BioTechniques 9:298-300.
4. Heyd, M.L. and J.D. Diefal, Jr. 1996. Recovering DNA from agarose gels with pumice. BioTechniques 20:394-398.
5. Noveen, A. and C.-M. Chuong. 1993. Design of compact multiple cloning sites. BioTechniques 75:208-214.
6. Stemmer, W.C., A. Crameri, K.D. Ha, T.M. Brennan and H.L. Heyneker. 1995. Single-step assembly of a gene and entire plasmid from large numbers of oligonucleotides. Gene 764:49-53.
7. Yanisch-Perron, C., J. Viera and J. Messing. 1985. Improved M13 phage cloning vectors and host stains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33:103- 119.