Purification of an antifungal PR-5 protein from flower buds of Brassica campestris and cloning of its gene

Physiologia Plantarum

Volumn 101, Issue 3, 1997, Pages 583-590

  Cheong, Na Eun ^a   Choi, Yeon Ok ^b   Kim, Woe Yeon ^a   Kim, Sun Chang ^c   Cho, Moo Je ^a,b   Lee, Sang Yeol ^a,b  

^a Gyeongsang National University   (South Korea)

^b Plant Molecular Biology   (South Korea)

^c Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Antifungal protein; Brassica campestris; Characterization; Gene cloning; PR 5 cDNA; Purification

Indexed keywords

BRASSICA CAMPESTRIS;

EID: 2642639644     PISSN: 00319317     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3054.1997.1010319.x     Document Type: Article

References (36)

1. Batalia, M. A., Monzingo, A. F., Ernst, S., Roberts, W. & Robertus, J. D. 1996. The crystal structure of the antifungal protein zeamatin, a member of the thaumatin-like, PR5 protein family. - Nat. Struct. Biol. 3: 19-23.
2. Bhakdi, S. & Tranum-Jensen, J. 1987. Damage to mammalian cells by proteins that form transmembrane pores. - Rev. Physiol. Biochem. Pharmacol. 107: 147-223.
3. Bohlmann, H. & Apel, K. 1991. Thionins. - Annu. Rev. Plant Physiol. Plant Mol. Biol. 42: 227-240.
4. Bowles, D. J. 1990. Defense-related proteins in higher plants. -Annu. Rev. Biochem. 59: 873-907.
5. Bradley, D. J., Kjellbom, P. & Lamb, C. J. 1992. Elicitor and wound induced oxidative cross-linking of a proline rich plant cell wall protein; A novel, rapid defense response. -Cell 70: 21-30.
6. Chomczynski, P. & Sacchi, N. 1987. Single step method of RNA isolation by acid guanidium thiocyanate phenol chloroform extraction. - Anal. Biochem. 162: 156-159.
7. Cornelissen, B. J. C., Hooft van Huijsduijnen, R. A. M. & Bol, J. F. 1986. A tobacco mosaic virus-induced tobacco protein is homologous to the sweet tasting protein thaumatin. - Nature 321: 531-532.
8. Dellaporta, S. L., Wood, J. & Hicks, J. B. 1983. A plant DNA minipreparation. Version II. - Plant Mol. Biol. Rep. 1: 19-22.
9. Edens, L., Hselinga, L., Klok, R., Ledeboer, A. M., Maat, J., Toonen, M. Y. Visser, C. & Verrips, C. 1982. Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in E. cali. - Gene 18: 1-12.
10. Eldik, G. J., Wingens, M., Ruiter, R. K., van Herpen, M. M. A., Schrauwen, J. A. M. & Wullems, G. J. 1996. Molecular analysis of a pistil-specific gene expressed in the stigma and stylar cortex of Solanum tuberosum. - Plant Mol. Biol. 30: 171-176.
11. Fraser, R. S. S. 1981. Evidence for the occurrence of the pathogenesis related proteins in leaves of healthy tobacco plants during flowering, - Physiol. Plant Pathol. 19: 69-76.
12. Hejgaard, J., Jacobsen, S. & Svendsen, I. 1991. Two antifungal thaumatin-like proteins from barely grain. - FEBS Lett. 291: 127-131.
13. Hewick, R. M., Hunkapiller, M. W., Hood, L. E. & Dreyer, W. J. 1981. A gas-liquid solid phase peptide and protein sequenator. - J. Biol. Chem. 256: 7990-7997.
14. Jacobsen, S., Mikkelsen, J. D. & Hejgaard, J. 1990. Characterization of two antifungal endochitinases from barley grain. -Physiol. Plant. 79: 554-562.
15. King, G. J., Turner, V. A., Hussey, C. E., Syrkin, W. E. & Lee, S. M. 1988. Isolation and characterization of a tomato cDNA clone which codes for a salt-induced protein. - Plant Mol. Biol. 10: 401-112.
16. Lagrimini, L. M., Burkhart, W., Moyer, M. & Rothstein, S. 1987. Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue specific expression. - Proc. Natl. Acad. Sci. USA 84: 7542-7546.
17. Liu, D., Raghothama, K. G., Hasegawa, P. M. & Bressan, R. A. 1994. Osmotin overexpression in potato delays development of disease symptoms. - Proc. Natl. Acad. Sci. USA 91: 1888-1892.
18. Lotan, T., Ori, N. & Fluhr, R. 1989. Pathogenesis-related proteins are developmentally regulated in tobacco flowers. -Plant Cell 1: 881-887.
19. Mackler, B. F. & Kreil, G. 1977. Honey bee venom melittin. -Inflammation 2: 55-65.
20. Melchers, L. S., Sela-Burrlage, M. B., Vloemans, S. A., Woloshuk, C. P., van Roekel, J. S. C., Pen, J., van den Elzen, P. J. M. & Cornelissen, B. J. C. 1993. Extracellular targeting of the vacuolar tobacco protein, AP24, chitinase and β-1,3 glucanase in transgenic plants. - Plant Mol. Biol. 21: 583-593.
21. Memelink, J., Hoge, J. H. C. & Schilperoort, R. A. 1987. Cyto- kinin stress changes the developmental regulation of several defence-related genes in tobacco. - EMBO J. 6: 3579-3583.
22. Nielsen, K. K., Mikkelsen, J. D., Kragh, K. M. & Bojsen, K. 1993. An acidic class III chitinase in sugar beet: Induction by Cercospora beticola, characterization, and expression in transgenic plants. - Mol. Plant-Microb. Interact. 6: 495-506.
23. Rebmann, G., Mauch, F. & Dudler, R. 1991. Sequence of a wheat cDNA encoding a pathogen induced thaumatin like protein. - Plant Mol. Biol. 17: 283-285.
24. Regalado, A. P. & Ricardo, C. P. P. 1996. Study of the intracellular fluid of healthy Lupinus albus organs. - Plant Physiol. 110: 227-232.
25. Richard, L., Arro, M., Hoebeke, J., Meeks-Wagner, D. R. & Tran Thanh Van, K. 1992. Immunological evidence of thaumatin-like proteins during tobacco floral differentiation. -Plant Physiol. 98: 337-342.
26. Richardson, M., Valdes-Rodriguez, S. & Blanco-Labra, A. 1987. A possible function for thaumatin and a TMV-induced protein suggested by homology to a maize inhibitor. - Nature 327: 432-434.
27. Roberts, W. K. & Selitrennikoff, C. P. 1990. Zeamatin, an antifungal protein from maize with membrane-permeabilizing activity. -J. Gen. Microbiol. 136: 1771-1778.
28. Rodrigo, I., Vera, P. Frank, R. & Conejero, V. 1991. Identification of the viroid-induccd tomato PR protein p23 as the thaumatin-like protein NP24 associated with osmotic stress. - Plant Mol. Biol. 16: 931-934.
29. Singh, N. K., Bracker, C. A., Hasegawa, P. M., Handa, A. K., Buckel, S., Hermodson, M. A., Pfankoch, E., Regnier, F. E. & Bressan, R. A. 1987. Characterization of osmotin, a thaumatin-like protein associated with osmotic adaption in plant cells. - Plant Physiol. 85: 529-536.
30. Takeda, S., Sato, F., Ida, K. & Yamada, Y. 1991. Nuclcotide sequence of a cDNA for osmotin-like protein from cultured tobacco cells. - Plant Physiol. 97: 844-846.
31. Towbin, H., Staehelin, T. & Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. - Proc. Natl. Acad. Sci. USA 76: 4350-4354.
32. Van Loon, L. C., Pierpoint, W. S., Boller, T. & Conjero, V. 1994. Recommendations for naming plant pathogenesis related pretcins. - Plant Mol. Biol. Rep. 12: 245-264.
33. Vlasak, R., Unger-Ullmann, C., Kreil, O. & Frischauf, A. M. 1983. Nuclcotide sequence of cloned cDNA coding for honeybee prepromelittin. - Eur. J. Biochem. 135: 123-126.
34. Vu, L. & Huynh, Q. K. 1994. Isolation and characterization of a 27 kD antifungal protein from the fruits of Diospyros texana. - Biochem. Biophys. Res. Commun. 202: 666-672.
35. Wang, X., Zafian, P., Choudhary, M. & Lawton, M. 1996. The PR5K receptor protein kinase from Arahidopsis thaliana is structurally related to a family of plant defense proteins. Proc. Natl. Acad. Sci. USA 93: 2598-2602.
36. Zasloff, M. 1987. Magainins, a class of antimicrobial peptides from Xenopus skin: Isolation, characterization of two active forms, and partial cDNA sequence of a precursor. - Proc. Natl. Acad. Sci. USA 84: 5449-5453.