The ferredoxin-encoding fdxN gene of the filamentous cyanobacterium Anabaena variabilis ATCC 29413 is not essential for nitrogen fixation

New Phytologist

Volumn 136, Issue 3, 1997, Pages 419-423

  Masepohl, Bernd ^a   Görlitz, Kirsten ^a   Monnerjahn, Ursula ^a   Mosler, Britta ^a   Böhme, Herbert ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

55 kb fdxN DNA element; Anabaena sp.; Bacterial type ferredoxin; Heterocysts; nifB fdxN nifS nifU operon

Indexed keywords

EID: 2642628710     PISSN: 0028646X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1469-8137.1997.00771.x     Document Type: Article

References (26)

1. Carrasco CD, Ramaswamy KS, Ramasubramanian TS, Golden JW. 1994. Anabaena xisF gene encodes a developmentally regulated site-specific recombinase. Genes & Development 8: 74-83.
2. Deng WP, Nickoloff JA. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Analytical Biochemistry 200: 81-88.
3. Elhai J, Wolk CP. 1988a. Conjugal transfer of DNA to cyanobacteria. Methods in Enzymology 167: 747-754.
4. Elhai J, Wolk CP. 1988b. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68: 119-138.
5. Golden JW, Carrasco CD, Mulligan ME, Schneider GJ, Haselkorn R. 1988. Deletion of a 55-kilobase-pair DNA element from the chromosome during heterocyst differentiation of Anabaena sp. strain PCC 7120. Journal of Bacteriology 170: 5034-5041.
6. Haselkorn R, Buikema WJ. 1992. Nitrogen fixation in cyano-bacteria. In: Stacey G, Burris RH, Evans HJ, eds. Biological Nitrogen Fixation. New York, London: Chapman and Hall, 166-190.
7. Jacobson BL, Chae YK, Böhme H, Markley JL, Holden HM. 1992. Crystallization and preliminary analysis of oxidized, recombinant, heterocyst [2Fe-2S] ferredoxin from Anabaena 7120. Archives of Biochemistry and Biophysics 294: 279-281.
8. Jouanneau Y, Meyer C, Naud I, Klipp W. 1995. Characterization of an fdxN mutant of Rhodobacter capsulatus indicates that ferredoxin I serves as electron donor to nitrogenase. Biochimica et Biophysica Acta 1232: 33-42.
9. Lyons EM, Thiel T. 1995. Characterization of nifB, nifS, and nifU genes in the cyanobacterium Anabaena variabilis: NifB is required for the vanadium-dependent nitrogenase. Journal of Bacteriology 177: 1570-1575.
10. Masepohl B, Klipp W, Pühler A. 1988. Genetic characterization and sequence analysis of the duplicated nifA/nifB gene region of Rhodobacter capsulatus. Molecular ana General Genetics 212: 27-37.
11. Mulligan ME, Buikema WJ, Haselkorn R. 1988. Bacterial-type ferredoxin genes in the nitrogen fixation regions of the cyanobacterium Anabaena sp. strain PCC 7120 and Rhizobium meliloti. Journal of Bacteriology 170: 4406-4410.
12. Mulligan ME, Haselkorn R. 1989. Nitrogen fixation (nif) genes of the cyanobacterium Anabaena species strain PCC 7120. The nifB-fdxN-nifS-nifU operon. The Journal of Biological Chemistry 264: 19200-19207.
13. Riedel K-U, Jouanneau Y, Masepohl B, Pühler A, Klipp W. 1995. A Rhizobium meliloti ferredoxin (FdxN) purified from Escherichia coli donates electrons to Rhodobacter capsulatus nitrogenase. European Journal of Biochemistry 231: 742-746.
14. Rippka R, Deruelles J, Waterbury JB, Herman M, Stanier RY. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111: 1-61.
15. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning. A laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
16. Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Acadamy of Sciences USA 74: 5463-5467.
17. Schrautemeier B, Böhme H. 1985. A distinct ferredoxin for nitrogen fixation isolated from heterocysts of the cyanobacterium Anabaena variabilis. Federation of European Biochemical Societies Letters 184: 304-308.
18. Schrautemeier B, Cassing A, Böhme H. 1994. Characterization of the genome region encoding an FdxH-type ferredoxin and a new 2[4Fe-4S] ferredoxin from the non-heterocystous, nitrogen-fixing cyanobacterium Plectonema boryanum PCC 73110. Journal of Bacteriology 176: 1037-1046.
19. Schrautemeier B, Neveling U, Schmitz S. 1995. Distinct and differently regulated Mo-dependent nitrogen-fixing systems evolved for heterocysts and vegetative cells of Anabaena variabilis ATCC 29413: characterization of the fdxH1/2 gene regions as part of the nif1/2 gene clusters. Molecular Microbiology 18: 357-369.
20. Smoker JA, Barnum SR. 1988. Rapid small-scale DNA isolation from filamentous cyanobacteria. FEMS Microbiology Letters 56: 119-122.
21. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology 185: 60-89.
22. Thiel T. 1993. Characterization of genes for an alternative nitrogenase in the cyanobacterium Anabaena variabilis. Journal of Bacteriology 175: 6276-6286.
23. Thiel T, Lyons EM, Erker JC, Ernst A. 1995. A second nitrogenase in vegetative cells of a heterocyst-forming cyanobacterium. Proceedings of the National Acadamy of Sciences USA 92: 9358-9362.
24. Thomson AJ. 1991. Does ferredoxin 1 (Azotobacter) represent a novel class of DNA-binding proteins that regulate gene expression in response to cellular iron(II)? Federation of European Biochemical Societies Letters 285: 230-236.
25. Wolk CP, Ernst A, Elhai J. 1994. Heterocyst metabolism and development. In: Bryant DA, ed. The Molecular Biology of Cyanobacteria. Dordrecht, Boston, London: Kluwer Academic Publishers, 769-823.
26. Yakunin AF, Hallenbeck PC, Troshina OY, Gogotov IN. 1993. Purification and properties of a bacterial-type ferredoxin from the nitrogen-fixing cyanobacterium Anabaena variabilis ATCC 29413. Biochimica et Biophysica Acta 1163: 124-130.