Application of the one-tube PCR technique in combination with a fast DNA extraction procedure for detecting Phytophthora infestans in infected potato tubers

Microbiological Research

Volumn 152, Issue 4, 1997, Pages 345-351

  Niepold, F ^a   Schober Butin B ^a  

^a INSTITUTE FOR EPIDEMIOLOGY AND PATHOGEN DIAGNOSTICS   (Germany)

Author keywords

Fast DNA extraction procedure; One tube PCR detection; Phytophthora infestans

Indexed keywords

BACTERIA (MICROORGANISMS); BOVINAE; FUNGI; PHYTOPHTHORA INFESTANS; SOLANUM TUBEROSUM;

EID: 2642612807     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0944-5013(97)80050-X     Document Type: Article

References (16)

1. Dellaporta, S. L., Wood, J. B. (1983): A plant DNA minipreparation: Version II. Plant Molecular Biology Reporter 1, 19-21.
2. Erwin, D. C., Ribeiro, O. K. (1996): Phytophthora diseases worldwide. APS Press, St. Paul, Minnesota, 562 pp.
3. Galindo, J., Hohl, H. R. (1985): Phytophthora mirabilis, a new species of Phytophthora. Sydowia 38, 87-96.
4. Henninger, H. (1959): Versuche zur Kultur verschiedener Rassen von Phytophthora infestans (Mont.) de Bary auf künstlichem Nährboden. Phytopathol. Zeitschrift 34, 285-306.
5. Henson, I. M., French, R. (1993): The polymerase chain reaction and plant disease diagnosis. Annu. Rev. Plant Pathol. 31, 81-109.
6. Knapova, G., Schöber-Butin, B., Fehrmann, H. (1992): Nachweis von P. infestans (Mont.) de Bary mittels ELISA. Mitt. Biol. Bundesanst. 283, 328
7. Knapova, G., Schöber-Butin, B., Fehrmann, H. (1993): Nachweis von P. infestans im Gewebe. Abstract 12th Triennial Conference of the European Association for Potato Research, Paris 467-468.
8. Möller, E. M. (1989): Repetitive DNA-Sequenzen von Phytophthora infestans (Montagne) de Bary: ihre Charakterisierung und Verwendung zur Konstruktion von DNA-Sonden für diagnostische und taxonomische Zwecke. Dissertation, Göttingen.
9. Niepold, F. (1994): Anwendung der Polymerase-Kettenreaktion (PCR) zum Nachweis von Erwinia carotovora ssp. atroseptica, dem Erreger der Schwarzbeinigkeit und Naßfäule bei Kartoffeln. Nachichtenbl. Deut. Pflanzenschutz. 46, 243-246.
10. Niepold, F., Schöber-Butin, B. (1995): Application of the PCR technique to detect Phytophthora infestans in potato tubers and leaves. Microbiol. Res. 150, 379-385.
11. Rhodes, M. E. (1959): The characterisation of Pseudomonas flourescens. J. Gen. Microbiol. 21, 221-263.
12. Rollo, F., Amici, A., Foresi, F., di Silvestro, I. (1987): Construction and characterization of a cloned probe for the detection of Phoma tracheiphila in plant tissue. Appl. Microbiol. Biotechnol. 26, 352-357.
13. Schesser, K., Luder, A., Henson, J. M. (1991): Use of Polymerase Chain Reaction to detect the take-all fungus, Gaeumannomyces graminis, in infected wheat plants. Appl. Environ. Microbiol. 57, 553-556.
14. Schöber, B., Höppner, E. (1972): Zur Methodik der Resistenzprüfung von Kartoffelknollen gegen den Erreger der Braunfäule, Phytophthora infestans (Mont.) de Bary. Potato Res. 15, 378-383.
15. Tooley, P. W., Bunyard, B. A., Carras, M. M., Hatziloukas, E. (1997): Development of PCR Primers from Internal Transcribed Spacer Region 2 for Detection of Phytophthora Species Infecting Potatoes. Appl. Environ. Microbiol. 63, 1467-1475.
16. Wang, H., Qi, M., Cutler, A. J. (1993): A simple method of preparing plant samples for PCR. Nucleic Acids Res. 21, 4153-4154.