The regulator of nitrate assimilation in ascomycetes is a dimer which binds a nonrepeated, asymmetrical sequence

Molecular and Cellular Biology

Volumn 18, Issue 3, 1998, Pages 1339-1348

  Strauss, Joseph ^a,b   Isabel Muro Pastor, M ^a   Scazzocchio, Claudio ^a  

^a UNIVERSITÉ PARIS SACLAY   (France)

^b VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; DNA; LEUCINE; NITRATE; TRANSCRIPTION FACTOR; ZINC;

ARTICLE; ASCOMYCETES; ASPERGILLUS NIDULANS; BINDING AFFINITY; CONTROLLED STUDY; DIMERIZATION; DNA PROTEIN COMPLEX; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING;

EID: 2642611985     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.18.3.1339     Document Type: Article

References (38)

1. Alber, T. 1992. Structure of the leucine zipper. Curr. Opin. Genet. Dev. 2:205-210.
2. Bailone, A., and F. Galimbert. 1980. Nucleotide sequence of the operators of λ ultravirulent mutants. Nucleic Acids Res. 8:2147-2164.
3. Battaglia, P. A., F. Longo, C. Ciotta, M. F. Del Grosso, E. Ambrosini, and F. Gigliani. 1994. Genetic tests to reveal Tat homodimer formation and select Tat homodimer inhibitor. Biochem. Biophys. Res. Commun. 201:701-708.
4. Burger, G., J. Tilburn, and C. Scazzocchio. 1991. Molecular cloning and functional characterization of the pathway-specific regulatory gene nirA, which controls nitrate assimilation in Aspergillus nidulans. Mol. Cell. Biol. 11:795-802.
5. Burger, G., J. Strauss, C. Scazzocchio, and B. F. Lang. 1991. nirA, the pathway-specific regulatory gene of nitrate assimilation in Aspergillus nidulans, encodes a putative GAL4-type zinc finger protein and contains four introns in highly conserved regions. Mol. Cell. Biol. 11:5746-5755.
6. Carey, M., H. Kakidani, J. Leatherwood, F. Mostashari, and M. Ptashne. 1989. An amino terminal fragment of GAL4 binds DNA as a dimer. J. Mol. Biol. 209:423-432.
7. Castagnoli, L., C. Vetriani, and G. Cesareni. 1994. Linking an easily detectable phenotype to the folding of a common structural motif. Selection of rare turn mutations that prevent the folding of Rop. J. Mol. Biol. 237:378-387.
8. Cerdan, R., D. Collin, F. Lenouvel, B. Felenbok, and E. Guittet. 1997. The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study. FEBS Lett. 408:235-240.
9. Cove, D. J., and J. A. Pateman. 1963. Independently segregating genetic loci concerned with nitrate reductase activity in Aspergillus nidulans. Nature 198:262-263.
10. Cubero, B., and C. Scazzocchio. 1994. Two different adjacent and divergent zinc-finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans. EMBO J. 13:407-415.
11. Ellenberger, T., D. Fass, M. Arnaud, and S. C. Harrison. 1994. Crystal structure of transcription factor E47: E-box recognition by a basic region helix-loop-helix dimer. Genes Dev. 8:970-980.
12. Fu, Y. H., B. Feng, S. Evans, and G. A. Marzluf. 1995. Sequence-specific DNA binding by NIT4, the pathway-specific regulatory protein that mediates nitrate induction in Neurospora. Mol. Microbiol. 15:935-942.
13. Halvorsen, Y. D. C., K. Nanadabalan, and R. C. Dickson. 1991. Identification of base and backbone contacts used for DNA sequence recognition and high-affinity binding by LAC9, a transcription activator containing a C6 zinc finger. Mol. Cell. Biol. 11:1777-1784.
14. Hellauer, K., M. H. Rochon, and B. Turcotte. 1996. A novel DNA binding motif for yeast zinc cluster proteins: the Leu3p and Pdr3p transcriptional activators recognize everted repeats. Mol. Cell. Biol. 16:6096-6102.
15. Hu, J. C., E. K. O'Shea, P. S. Kim, and R. T. Sauer. 1990. Sequence requirements for coiled-coils: analysis with X repressor-GCN4 leucine zipper fusions. Science 250:1400-1403.
16. Kopka, M. L., C. Yoon, D. Goodsell, P. Pjura, and R. Dickerson. 1985. The molecular origin of DNA-drug specificity in netropsin and distamycin. Proc. Natl. Acad. Sci. USA 82:1376-1380.
17. Kulmburg, P., T. Prangé, M. Mathieu, D. Sequeval, C. Scazzocchio, and B. Felenbok. 1991. Correct intron splicing generates a new type of a putative zinc-binding domain in a transcriptional activator of Aspergillus nidulans. FEBS Lett. 280:11-16.
18. Kulmburg, P., D. Sequeval, F. Lenouvel, M. Mathieu, and B. Felenbok. 1992. Identification of the promoter region involved in the autoregulation of the transcriptional activator ALCR in Aspergillus nidulans. Mol. Cell. Biol. 12:1932-1939.
19. Kulmburg, P., N. Judewicz, M. Mathieu, F. Lenouvel, D. Sequeval, and B. Felenbok. 1992. Specific binding sites for the activator protein ALCR, in the alcA promoter of the ethanol regulon of Aspergillus nidulans. J. Biol. Chem. 267:21146-21153.
20. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
21. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
22. Lenouvel, F., I. Nikolaev, and B. Felenbok. 1997. In vitro recognition of specific DNA targets by AlcR, a zinc binuclear cluster activator different from the other proteins of this class. J. Biol. Chem. 272:15521-15526.
23. 6 binuclear cluster proteins recognizes DNA. Mol. Cell. Biol. 16:3773-3780.
24. Marmorstein, R., M. Carey, M. Ptashne, and S. C. Harrison. 1992. DNA recognition by GAL4: structure of a protein-DNA complex. Nature (London) 356:408-415.
25. 6 binuclear cluster. Genes Dev. 8:2504-2512.
26. Maxam, A. M., and W. Gilbert. 1980. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 65:497-559.
27. Panozzo, C., V. Capuano, S. Fillinger, and B. Felenbok. 1997. The zinc binuclear cluster activator AlcR is able to bind to single sites, but requires multiple repeated sites for synergistic activation of the alcA gene in Aspergillus nidulans. J. Biol. Chem. 272:22859-22865.
28. Punt, P. J., J. Strauss, R. Smit, J. R. Kinghorn, C. A. M. J. J. van den Hondel, and C. Scazzocchio. 1995. The intergenic region between the divergently transcribed niiA and niaD genes of Aspergillus nidulans contains multiple NirA binding sites which act bidirectionally. Mol. Cell. Biol. 15:5688-5699.
29. 6 zinc cluster proteins. Science 261:909-911.
30. Schjerling, P., and S. Holmberg. 1996. Comparative amino acid sequence analysis of the C6 zinc cluster family of transcriptional regulators. Nucleic Acids Res. 24:4599-4607.
31. Strauss, J. 1993. Bases moléculaires de la régulation des gènes de la voie d'assimilation du nitrate chez Aspergillus nidulans. Ph.D. thesis. Université Paris Sud, Centre d'Orsay, Orsay, France.
32. Suárez, T., M. Vieira de Queiroz, N. Oestreicher, and C. Scazzocchio. 1995. The sequence and binding specificity of UaY, the specific regulator of the purine utilization pathway in Aspergillus nidulans, suggest an evolutionary relationship with the PPR1 protein of Saccharomyces cerevisiae. EMBO J. 14:1453-1467.
33. 6 hinuclear cluster. Nat. Struct. Biol. 4:751-759.
34. Unkles, S. E., K. L. Hawker, C. Grieve, E. I. Campbell, P. Montague, and J. R. Kinghorn. 1991. crnA encodes a nitrate transporter in Aspergillus nidulans. Proc. Natl. Acad. Sci. USA 88:204-208.
35. Wolschek, M., F. Narendja, J. Karlseder, B. Cubero, C. Scazzocchio, C. P. Kubicek, and J. Strauss. Unpublished data.
36. Yuan, G. F., Y. H. Fu, and G. A. Marzluf. 1991. nit4, a pathway-specific regulatory gene of Neurospora crassa, encodes a protein with a putative binuclear zinc DNA-binding domain. Mol. Cell. Biol. 11:5735-5745.
37. Zhang, L., and L. Guarente. 1994. The yeast activator HAP1 - a GALA family member - binds DNA in a directly repeated orientation. Genes Dev. 8:2110-2119.
38. Zhang, L., and L. Guarente. 1996. The C6 zinc cluster dictates asymmetric binding by HAP1. EMBO J. 15:4676-4681.