Characterization of the differentiated phenotype of an organotypic model of skin derived from human keratinocytes and dried porcine dermis

Folia Biologica

Volumn 44, Issue 2, 1998, Pages 59-66

  Matouskova E ^a   McKay, I ^b   Povysil C ^c   Konigova R ^c   Chaloupkova A ^a   Vesely P ^a  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

^b QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^c CHARLES UNIVERSITY   (Czech Republic)

Author keywords

Differentiated epidermis; Keratinocytes; Organotypic culture; Skin model

Indexed keywords

INTEGRIN; INVOLUCRIN; KERATIN; MONOCLONAL ANTIBODY; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE;

ANIMAL TISSUE; ANTIGEN EXPRESSION; ARTICLE; CELL STRAIN 3T3; DERMIS; EPIDERMIS; EXPERIMENTAL MODEL; HUMAN; HUMAN CELL; KERATINOCYTE; NONHUMAN; ORGAN CULTURE; SKIN; STRATUM CORNEUM; SWINE; TISSUE DIFFERENTIATION;

EID: 2642607040     PISSN: 00155500     EISSN: 25337602     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Akiyama, S. K., Yamada, S. S., Chen, W. T., Yamada, K. M. (1989) Analysis of fibronectin receptor function with monoclonal antibodies: roles in cell adhesion, migration, matrix assembly and cytoskeletal organization. J. Cell Biol. 109, 863-875.
2. Asselineau, D., Pruniéras, M. (1984) Reconstruction of 'simplified' skin: control of fabrication. Br. J. Dermatol. 111, 219-222.
3. Asselineau, D., Bernard, A. B., Bailly, C., Darmon, M., Pruniéras, M. (1986) Human epidermis reconstructed by culture: is it "normal"? J. Invest. Dermatol. 86, 181-186.
4. Bártek, J., Vojtěšek, B., Stašková, Z., Bártková, J., Kerekes, Z., Rejtar, A., Kovařík, J. (1991) A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J. Pathol. 164, 215-224.
5. Bell, E., Ehrlich, H. P., Buttle, D. J., Nakatsuji, T. (1981) Living tissue formed "in vitro" and accepted as skin equivalent tissue of full thickness. Science 211, 1052-1054.
6. Bell, E., Parenteau, N., Gay, R., Nolte, C., Kemp, P., Bilbo, P., Ekstein, B., Johnson, E. (1991) The living skin equivalent, its manufacture, its organotypic properties and its responses to irritants. Toxic in vitro 5, 591-96.
7. Bessou, S., Surlève-Bazeille, J. E., Pain, C., Donatien, P., Taieb, A. (1996) Ex vivo study of skin phototypes. J. Invest. Dermatol. 107, 684-688.
8. Contard, P., Bartel, R. L., Jacobs, L., Perlish, J. S., MacDonald, E. D., Handler, L., Cone, D., Fleischmajer, R. (1993) Culturing keratinocytes and fibroblasts in a three-dimensional mesh results in epidermal differentiation and formation of a basal lamina-anchoring zone. J. Invest. Dermatol. 100, 35-39.
9. Dollard, C. D., Wilson, J. R., Demeter, L. M., Bonnez, W. (1992) Production of human papillomavirus and modulation of the infectious program in epithelial raft cultures. Genes Devel. 6, 1131-1142.
10. Fartasch, M., Ponec, M. (1994) Improved barrier structure formation in air-exposed human keratinocyte culture systems. J. Invest. Dermatol. 102, 366-374.
11. Fusening, N. E. (1994) Epithelial-mesenchymal interactions regulate keratinocyte growth and differentiation in vitro. In: The Keratinocyte Handbook, eds. Leigh, I. M., Lane, E. B., Watt, F. M., pp.71-94. Cambridge University Press, Cambridge.
12. Gay, R., Swiderek, M., Nelson, D., Ernesti, A. (1992) The living skin equivalent as a model in vitro for ranking the toxic potential of dermal irritants. Toxic in vitro 6, 305-315.
13. Hansbrough, J. F., Boyce, S. T., Cooper, M. L., Foreman, T. J. (1989) Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate. JAMA 262, 2125-2130.
14. Holden, C. A., MacDonald, D. M. (1983) Immunoperoxidase techniques in dermatopathology. Clin. Exp. Dermatol. 8, 433-457.
15. Hudson, D. L., Weiland, K. L., Dooley, T. P., Simon, M., Watt, F. M. (1992) Characterization of eight monoclonal antibodies to involucrin. Hybridoma 11, 367-379.
16. Kopan, R., Traska, G., Fuchs, E. (1987) Retinoids as important regulators of terminal differentiation: examining keratin expression in individual epidermal cells at various stages of keratinization. J. Cell Biol. 105, 427-440.
17. Lane, E. B., Bártek, J., Purkis P. E., Leigh, I. M. (1985) Keratin antigens in differentiating skin. Ann. N. Y. Acad. Sci. 455, 241-248.
18. Leigh, I. M., Watt, F. M. (1994) The culture of human epidermal keratinocytes. In: The Keratinocyte Handbook, eds. Leigh, I. M., Lane, E. B., Watt, F. M., pp.43-51. Cambridge University Press, Cambridge.
19. Leigh, I. M., Purkis, P. E., Bruckner-Tuderman, L. (1988) LH 7:2 monoclonal antibody detects type VII collagen in the basement membrane of ectodermally derived epithelia including skin. Epithelia 1, 17-29.
20. Leigh, I. M., Navsaria, H., Purkis, P. E., McKay, I. A., Bowden, P. E., Riddle, P. N. (1995) Keratins (K16 and K17) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro. Br. J. Dermatol. 133, 501-511.
21. Markey, A. C., Lane, E. B., MacDonald, D. M., Leigh, I. M. (1992) Keratin expression in basal cell carcinomas. Br. J. Dermatol. 126, 154-160.
22. Matoušková, E., Vogtová, D., Königová, R. (1993) A recombined skin composed of human keratinocytes cultured on cell-free pig dermis. Burns 19, 118-123.
23. Matoušková, E., Němcová, D., Dvořanková, B., Vogtová, D., Königová, R. (1994) Healing effect of the recombined human/pig skin. Ann. Mediter. Burns Club 7, 143-146.
24. Matoušková, E., Buček, S., Vogtová, D., Veselý, P., Chaloupková, A., Brož, L., Singerová, H., Pavlíková, L., Königová, R. (1997) Treatment of burns and donor sites with human allogeneic keratinocytes grown on acellular pig dermis. Br. J. Dermatol. 136, 901-907.
25. McGrath, J. A., Leigh, I. M., Eady, R. A. J. (1992) Intracellular expression of type VII collagen during wound healing in severe recessive dystrophic epidermolysis bullosa and normal human skin. Br. J. Dermatol. 127, 312-317.
26. Medalie, D. A., Eming, S. A., Tompkins, R. G., Yarmush, M. L., Krueger, G. G., Morgan, J. R. (1996) Evaluation of human skin reconstituted from composite grafts of cultured keratinocytes and human acellular dermis transplanted to athymic mice. J. Invest. Dermatol. 107, 121-127.
27. Ponec, M. (1991) Reconstruction of human epidermis on deepidermized dermis: expression of differentiation specific protein markers and lipid composition. Toxic in vitro 5, 597-606.
28. Pruniéras, M., Régnier, M., Woodley, D. (1983) Methods for cultivation of keratinocytes with an air-liquid interface. J. Invest. Dermatol. 81, 28s-33s.
29. Purkis, P. E., Steel, J. B., MacKenzie, I. C., Nathrath, W. B. J., Leigh, I. M., Lane, E. B. (1990) Antibody markers of basal cells in complex epithelia. J. Cell Sci. 97, 39-50.
30. Régnier, M., Pruniéras, M., Woodley, D. (1981) Growth and differentiation of adult human epidermal cells on dermal substrates. Front Matrix Biology 9, 4-35.
31. Rheinwald, J. G., Green, H. (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from the single cells. Cell 6, 331-344.
32. Slivka, S., Landeen, L. K., Zeigler, F., Zimber, M., Bartel, R. L. (1993) Characterization, barrier function, and drug metabolism of an in vitro skin model. J. Invest. Dermatol. 100, 40-46.
33. Stanley, M. A. (1994) Replication of human papillomaviruses in cell culture. Antiviral Res. 24, 1-15.
34. Thacher, S. M., Rice, R. H. (1985) Keratinocyte-specific transglutaminase of cultured human epidermal cells; relation to cross-linked envelope formation and terminal differentiation. Cell 40, 685-695.