Demonstration of protein-protein interaction specificity by NMR chemical shift mapping

Protein Science

Volumn 6, Issue 12, 1997, Pages 2624-2627

  Rajagopal, Ponni ^a   Bruce Waygood, E ^b   Reizer, Jonathan ^c   Saier Jr , Milton H ^c   Klevit, Rachel E ^a,d  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF SASKATCHEWAN   (Canada)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Binding specificity; Chemical shift mapping; NMR; Phosphoenolpyruvate:sugar phosphotransferase system; Protein protein interactions

Indexed keywords

BACTERIAL ENZYME; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE;

ARTICLE; BACILLUS SUBTILIS; ESCHERICHIA COLI; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 2642605367     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560061214     Document Type: Article

References (11)

1. Bax A, Ikwia M, Kay LE, Torchia DA, Tschudin R. 1990. Comparison of different modes of two-dimensional reverse-correlation NMR for the study of proteins. J Magn Reson 86:304-318.
2. Chen Y, Reizer J, Saier MH Jr, Fairbrother WJ, Wright PE. 1993. Mapping of the binding interfaces of the proteins of the bacterial phosphotransferase system. HPr and IIAglc. Biochemistry 32:32-37.
3. Hammen PK, Waygood EB, Klevit RE. 1991. Reexamination of the secondary and tertiary structure of histidine-containing protein from Escherichia coli by homonuclear and heteronuclear NMR spectroscopy. Biochemistry 30: 11842-11850.
4. Herzberg O, Klevit RE. 1994. Unraveling a bacterial hexose transport pathway. Curr Opin Struct Biol 4:814-822.
5. Jones BE, Rajagopal P, Klevit RE. 1997. Phosphorylation on histidine is accompanied by localized structural changes in the phosphocarrier protein from Bacillus subtilis, HPr. Protein Sci 6:2107-2119.
6. Meadow ND, Roseman S. 1982. Sugar transport by the bacterial phosphotransferase system. Isolation and characterization of a glucose-specific phosphocarrier protein (IIIGlc) from Salmonella typhimurium. J Biol Chem 257: 14526-14537.
7. Rajagopal P, Waygood EB, Klevit RE. 1994. Structural consequences of histidine phosphorylation: NMR characterization of the phosphohistidine form of histidine-containing protein from Bacillus subtilis and Escherichia coli. Biochemistry 33:15271-15282.
8. Reizer J, Sutrina SL, Wu LF, Deutscher J, Reddy P, Saier MH Jr. 1992. Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli. J Biol Chem 267:9158-9169.
9. Sutrina SL, Reddy P, Saier MH Jr, Reizer J. 1990. The glucose permease of Bacillus subtilis is a single polypeptide chain that functions to energize the sucrose permease. J Biol Chem 265:18581-18589.
10. van Nuland NA, Kroon GJ, Dijkstra K, Wolters GK, Scheek RM, Robillard GT. 1993. The NMR determination of the IIA(mtl) binding site on HPr of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system. FEBS Lett 315:11-15.
11. 1H NMR resonance assignments of Bacillus subtilis HPr: Use of spectra obtained from mutants to resolve spectral overlap. Biochemistry 29:7191-7200.