Artifact occurring during combined in situ hybridization and immunocytochemistry analysis because of positive chemography

BioTechniques

Volumn 24, Issue 5, 1998, Pages 730-732

  Häkkinen, Tomi ^a   Ylä Herttuala, Seppo ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

APOLIPOPROTEIN E;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL STRUCTURE; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; NONHUMAN; RABBIT; THORACIC AORTA;

EID: 2642600055     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98245bm06     Document Type: Article

References (5)

1. Griffin, W.S.T and A.T. Haase. 1987. What is the nature of some of the common artifacts encountered in doing in situ hybridizations? p. 221. In K.L. Valentine, J.H. Eberwine and J.D. Barchas (Eds.), In Situ Hybridization: Applications to Neurobiology. Oxford University Press, New York.
2. Rosenfeld, M.E., S. Butler, V.A. Ord, B.A. Lipton, C.A. Dyer, L.K. Curtiss, W. Palinski and J.L. Witztum. 1993. Abundant expression of apoprotein E by macrophages in human and rabbit atherosclerotic lesions. Arterioscler. Thromb. 13:1382-1389.
3. Ylä-Herttuala, S., M.E. Rosenfeld, S. Parthasarathy, C.K. Glass, E. Sigal, J.L. Witztum and D. Steinberg. 1990. Colocalization of 15-lipoxygenase mRNA and protein with epitopes of oxidized low density lipoprotein in macrophage-rich areas of atherosclerotic lesions. Proc. Natl. Acad. Sci. USA 87:6959-6963.
4. Young, W.S., III and A.C. Hsu. 1991. Observations on the simultaneous use of digoxigenin- and radiolabeled oligodeoxyribonucleotide probes for hybridization histochemistry. Neuropeptides 18:15.
5. Zannis, V.I., J. McPherson, G. Goldberger, S.K. Karathanasis and J.L. Breslow. 1984. Synthesis, intracellular processing, and signal peptide of human apolipoprotein E. J. Biol. Chem. 259:5495-5499.