Improved method for the isolation of RNA from (standing liquid cultures of) Streptomycetes

Journal of Microbiological Methods

Volumn 58, Issue 1, 2004, Pages 139-142

  Van Keulen, Geertje ^a   Siebring, Jeroen ^a   Rembacz, Krzysztof P ^a   Hoogeveen, Martin ^a   Tomczynska, Magdalena ^a   Dijkhuizen, Lubbert ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Efficient lysis; RNA isolation; Streptomyces

Indexed keywords

BACTERIAL RNA;

ARTICLE; BACTERIAL CELL; BACTERIUM CULTURE; BIOTECHNOLOGICAL PROCEDURES; CONTROLLED STUDY; CYTOLYSIS; LIFE CYCLE; LIQUID CULTURE; NONHUMAN; PRIORITY JOURNAL; RNA ISOLATION; STREPTOMYCES COELICOLOR; STREPTOMYCETACEAE;

BLOTTING, NORTHERN; DEOXYRIBONUCLEASE I; GUANIDINES; ISOTHIOCYANATES; PHENOL; RNA, BACTERIAL; SPORES, BACTERIAL; STREPTOMYCES;

STREPTOMYCES; STREPTOMYCINEAE;

EID: 2642567490     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2004.03.014     Document Type: Article

References (8)

1. Chater K.F., Horinouchi S. Signalling early development events in two highly diverged Streptomyces species. Mol. Microbiol. 48:2003;9-15
2. Church G.M., Gilbert W. Genomic sequencing. Proc. Natl. Acad. Sci. U. S. A. 81:1984;1991-1995
3. Hopwood D.A. Fourty years of genetics with Streptomyces: from in vivo through in vitro to in silico. Microbiology. 145:1999;2183-2202
4. Kieser T., Bibb M.J., Buttner M.J., Chater K.F., Hopwood D.A. Practical Streptomyces Genetics. 2000;John Innes Foundation, Norwich
5. Kormanec J., Farkašovský M. Isolation of total RNA from yeast and bacteria and detection of rRNA in northern blots. BioTechniques. 17:1994;839-842
6. La Farina M., Sollazzo M., Puglia A.M. A procedure to purify RNA and DNA molecules from Streptomyces coelicolor A3(2). Cell Biol. Int. Rep. 7:1983;311-316
7. Van Keulen G., Jonkers H.M., Claessen D., Dijkhuizen L., Wösten H.A.B. Differentiation and anaerobiosis in standing liquid cultures of Streptomyces coelicolor. J. Bacteriol. 185:2003;1455-1458
8. Veenendaal A.K.J., Wösten H.A.B. Total RNA extraction from the Gram-positive bacterium Streptomyces lividans using the SV Total RNA Isolation System. Promega Benelux News. 17:1998;7