Dose dependency of FISH-detected translocations in stable and unstable cells after 137Cs γ irradiation of human lymphocytes in vitro

Cytogenetic and Genome Research

Volumn 104, Issue 1-4, 2004, Pages 162-167

  Romm, H ^a   Stephan, G ^a  

^a BUNDESAMT FÜR STRAHLENSCHUTZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CESIUM 137;

CELLULAR DISTRIBUTION; CHROMOSOME ANALYSIS; CHROMOSOME TRANSLOCATION; CONFERENCE PAPER; CONTROLLED STUDY; DOSE RESPONSE; FLUORESCENCE IN SITU HYBRIDIZATION; GAMMA IRRADIATION; HUMAN; HUMAN CELL; LYMPHOCYTE; MATHEMATICAL MODEL; POISSON DISTRIBUTION; PRIORITY JOURNAL; RADIATION DOSE; RADIATION DOSE DISTRIBUTION; RADIATION EXPOSURE; RADIATION RESPONSE;

ALGORITHMS; CALIBRATION; CELLS, CULTURED; CESIUM RADIOISOTOPES; CHROMOSOMAL INSTABILITY; CHROMOSOME ABERRATIONS; CHROMOSOME BREAKAGE; CHROMOSOME PAINTING; CHROMOSOMES, HUMAN; CHROMOSOMES, HUMAN, PAIR 2; CHROMOSOMES, HUMAN, PAIR 4; CHROMOSOMES, HUMAN, PAIR 8; DOSE-RESPONSE RELATIONSHIP, RADIATION; GAMMA RAYS; HUMANS; LINEAR ENERGY TRANSFER; LYMPHOCYTES; MALE; MIDDLE AGED; RADIATION TOLERANCE; TRANSLOCATION, GENETIC;

EID: 2642552226     PISSN: 14248581     EISSN: None     Source Type: Journal    
DOI: 10.1159/000077482     Document Type: Conference Paper

References (22)

1. Bauchinger M, Schmid E, Zitzelsberger H, Braselmann H, Nahrstedt U: Radiation-induced chromosome aberrations analysed by two-colour fluorescence in situ hybridization with composite whole chromosome-specific DNA probes and a pancentromeric DNA probe. Int J Radiat Biol 64:179-184 (1993).
2. Buckton KE, Pike MC: Time in culture - an important variable in studying in vivo radiation-induced chromosome damage in man. Int J Radiat Biol 8:439-452 (1964).
3. 60Co gamma ray dose-response for chromosomal aberrations in human lymphocytes analysed by FISH; applicability to biological dosimetry. Int J Radiat Biol 75:1215-1222 (1999).
4. Guerrero-Carbajal YC, Moquet JE, Edwards AA, Lloyd DC: The persistence of FISH translocations for retrospective biological dosimetry after simulated whole or partial body irradiation. Radiat Prot Dosim 76:159-168 (1998).
5. 60Co gamma rays at body temperature. Int J Radiat Biol 75:435-439 (1999).
6. Kulka U, Huber R, Muller P, Knehr S, Bauchinger M: Combined FISH painting and harlequin staining for cell cycle-controlled chromosome analysis in human lymphocytes. Int J Radiat Biol 68:25-27 (1995).
7. Lindholm C, Luomahaara S, Koivistoinen A, Ilus T, Edwards AA, Salomaa S: Comparison of doseresponse curves for chromosomal aberrations established by chromosome painting and conventional analysis. Int J Radiat Biol 74:27-34 (1998).
8. Lindholm C, Romm H, Stephan G, Schmid E, Moquet J, Edwards A: Intercomparison of translocation and dicentric frequencies between laboratories in a follow-up of the radiological accident in Estonia. Int J Radiat Biol 78:883-890 (2002).
9. Lucas JN: Dose reconstruction for individuals exposed to ionizing radiation using chromosome painting. Radiat Res 148:S33-38 (1997).
10. Lucas JN, Awa A, Straume T, Poggensee M, Kodama Y, Nakamo M, Ohtaki K, Weier HU, Pinkel D, Gray J, Littlefield G: Rapid translocation frequency analysis in humans decades after exposure to ionizing radiation. Int J Radiat Biol 62:53-63 (1992).
11. 60Co gamma rays. Health Phys 68:761-765 (1995).
12. Lucas JN, Chen AM, Sachs RK: Theoretical predictions on the equality of radiation-produced dicentrics and translocations detected by chromosome painting. Int J Radiat Biol 69:145-153 (1996).
13. 137Cs gamma rays. Radiat Prot Dosim 71:279-282 (1997).
14. Matsumoto K, Ramsey MJ, Nelson DO, Tucker JD: Persistence of radiation-induced translocations in human peripheral blood determined by chromosome painting. Radiat Res 149:602-613 (1998).
15. Morton NE: Parameters of the human genome. Proc natl Acad Sci, USA 88:7474-7476 (1991).
16. 137Cesium-induced chromosome aberrations analyzed by fluorescence in situ hybridization: eight years follow-up of the Goiania radiation accident victims. Mutat Res 400:299-312 (1998).
17. Papworth DG: in Appendix to paper by J.R.K. Savage, Sites of radiation-induced chromosome exchanges. Current Topics Radiat Res 6:129-194 (1970).
18. Pressl S, Edwards A, Stephan G: The influence of age, sex and smoking habits on the background level of FISH-detected translocations. Mutat Res 442:89-95 (1999).
19. Savage JRK, Simpson PJ: FISH "painting" patterns resulting from complex exchanges. Mutat Res 312:51-60 (1994).
20. Stephan G, Pressl S: Chromosome aberrations in human lymphocytes analysed by fluorescence in situ hybridization after in vitro irradiation, and in radiation workers, 11 years after an accidental radiation exposure. Int J Radiat Biol 71:293-299 (1997).
21. Tucker JD, Morgan WF, Awa AA, Bauchinger M, Blakey D, Cornforth MN, Littlefield LG, Natarajan AT, Shasserre C: PAINT: a proposed nomenclature for structural aberrations detected by whole chromosome painting. Mutat Res 347:21-24 (1995).
22. Tucker JD, Ramsey MJ, Lee DA, Minkler JL: Validation of chromosome painting as a biodosimeter in human peripheral lymphocytes following acute exposure to ionizing radiation in vitro. Int J Radiat Biol 64:27-37 (1993).