Co-ordinate expression of β- And δ-zeins in transgenic tobacco

Plant Science

Volumn 167, Issue 2, 2004, Pages 367-372

  Randall, Jennifer ^a   Sutton, Dennis ^a   Ghoshroy, Soumitra ^a   Bagga, Suman ^a   Kemp, John D ^a  

^a NEW MEXICO STATE UNIVERSITY   (United States)

Author keywords

2A polyprotein; Co ordinate expression; Protein bodies; Transgenic plants; Zein

Indexed keywords

ALCOHOLS; AMINO ACIDS; PLANTS (BOTANY); PROTEINS; SEED; SOLUBILITY; SULFUR; SYNTHESIS (CHEMICAL);

ENDOSPERM; SEED STORAGE PROTEINS;

TOBACCO;

NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; ZEA MAYS;

EID: 2642543397     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plantsci.2004.04.005     Document Type: Article

References (17)

1. Donnelly M.L., Luke G., Mehrotra A., Li X., Hughes L.E., Gani D., Ryan M.D. Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'. J. Gen. Virol. 82:2001;1013-1025
2. Gianazza E., Viglienghi V., Righetti P.B., Salamini F., Soave C. Amino acid composition of zein molecular components. Phytochemistry. 16:1977;315-317
3. Bagga S., Adams H., Kemp J.D., Sengupta-Gopalan C. Accumulation of 15-kilodalton zein in novel protein bodies in transgenic tobacco. Plant Physiol. 107:1995;13-23
4. Lending C.R., Larkins B.A. Changes in the zein composition of protein bodies during maize endosperm development. Plant Cell. 1:1989;1011-1023
5. Bagga S., Adams H.P., Rodriquez F.D., Kemp J.D., Sengupta-Gopalan C. Co-expression of the maize delta zein and beta zein genes results in stable accumulation of delta-zein in endoplasmic reticulum derived protein bodies formed by beta-zein. Plant Cell. 9:1997;1683-1696
6. Randall J., Bagga S., Adams H., Kemp J.D. A modified 10 kD zein protein produces two morphologically distinct protein bodies in transgenic tobacco. Plant Sci. 150:2000;21-28
7. Hinchliff D.J., Kemp J.D. Beta zein protein bodies sequester and protect 18 kD δ-zein protein from degradation. Plant Sci. 163:2002;741-752
8. Halpin C., Askari B.M., Abbot J.C., Ryan M.D. Enabling technologies for manipulating multiple genes on complex pathways. Plant Mol. Biol. 47:2001;295-310
9. Halpin C., Cooke S.E., Barakate A., Amrani A.E., Ryan M.D. Self-processing 2A-polyproteins - a system for co-ordinate expression of multiple proteins in transgenic plants. Plant J. 17(4):1999;453-459
10. Rogers S., Klee H., Horsch R., Fraley R. Improved vectors for plant transformation: expression cassette vectors and new identifiable markers. Methods Enzymol. 153:1987;253-263
11. Horsch R.B., Fry F.E., Hoffmann N.L., Eichholtz D., Rogers S.G., Fraley R.T. A simple and general method for transferring genes into plants. Science. 227:1985;1229-1231
12. deVries S., Springer J., Wessels J. Diversity of abundant mRNA sequences and patterns of protein synthesis in etiolated and greened pea seedlings. Planta. 156:1982;129-135
13. Boehringer Mannheim, The Genius™ System Nonradioactive Nucleic Acid Detection Kit, 1995.
14. Laemmli U. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 277:1970;680-685
15. Kiraihara J.A., Hunsperger J.P., Mahoney W.C., Messing J.W. Differential expression of a gene for a methionine-rich storage protein in maize. Mol. Gen. Genet. 211:1988;477-484
16. J. Randall, Targeting and Stabilization of 10 kD and 15 kD Zeins in Transgenic Tobacco, NMSU, 1997.
17. Ghoshroy S., Citovsky V. Preservation of plant cell ultrastructure during immunolocalization of virus particles. J. Virol. Methods. 74:1998;223-229