An electrophoretic profiling method for thiol-rich phytochelatins and metallothioneins

Phytochemical Analysis

Volumn 15, Issue 3, 2004, Pages 175-183

  Fan, Teresa W M ^a,b   Lane, Andrew N ^b   Higashi, Richard M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF LOUISVILLE   (United States)

Author keywords

Cadmium; HPLC tandem MS; Monobromobimane; NMR; Rice; Thiol rich peptides; Tricine SDS urea PAGE; Wheat

Indexed keywords

CADMIUM; CHELATION; CHROMOPHORES; DETOXIFICATION; ELECTROPHORESIS; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SODIUM COMPOUNDS; SULFUR COMPOUNDS; UREA;

HPLC-TANDEM MS; METALLOTHIONEINS; MONOBROMOBIMANE; PHYTOCHELATINS; PROFILING METHODS; RICE; TANDEM MS; THIOL-RICH PEPTIDE; TRICINE-SDS-UREA PAGE; WHEAT;

PEPTIDES;

BROMOBIMANE; DODECYL SULFATE SODIUM; GLUTATHIONE; METALLOTHIONEIN; PHYTOCHELATIN; THIOL DERIVATIVE; UREA;

ARTICLE; CHROMATOPHORE; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; MOLECULAR SIZE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PEPTIDE ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN MODIFICATION; SEPARATION TECHNIQUE; TANDEM MASS SPECTROMETRY;

TRITICUM AESTIVUM;

EID: 2642522041     PISSN: 09580344     EISSN: None     Source Type: Journal    
DOI: 10.1002/pca.765     Document Type: Article

References (40)

1. Abrahamson SL, Speiser DM, Ow DW. 1992. A gel electrophoresis assay for phytochelatins. Anal Biochem 200: 239-243.
2. Ahner BA, Price NM, Morel FMM. 1994. Phytochelatin production by marine phytoplankton at low free metal ion concentrations: Laboratory studies and field data from Massachusetts Bay. Proc Natl Acad Sci USA 91: 8433-8436.
3. Ahner BA, Kong S, Morel FMM. 1995. Phytochelatin production in marine algae: 1. An interspecies comparison. Limnol Oceanogr 40: 649-657.
4. Bienengraeber M, Forderkunz S, Klein D, Summer KH. 1995. Determination of Cu-containing metallothionein: Comparison of Ag saturation assay, thiomolybdate assay, and enzyme-linked immunosorbent assay. Anal Biochem 228: 69-73.
5. Bremner I, Beattie HH. 1990. Metallothionein and the trace minerals. A Rev Nutr 10: 63-84.
6. Chassaigne H, Lobinski R. 1998. Speciation of metal complexes with biomolecules by reversed-phase HPLC with ion-spray and inductively coupled plasma mass spectrometric detection. Fresenius J Anal Chem 361: 267-273.
7. Cizewski Culotta VC, Howard WR, Liu XF. 1994. CRS5 encodes a metallothionein-like protein in Saccharomyces cerevisiae. J Biol Chem 269: 25295-25302.
8. Clemens S, Schroeder JI, Degenkolb T. 2001. Caenorhabditis elegans expresses a functional phytochelatin synthase. Eur J Biochem 268: 3640-3643.
9. Danielsohn P, Nolte A. 1987. Bromobimanes: fluorescent labelling agents for histochemical detection of sulphur containing neuropeptides in semi-thin sections. Histochem 86: 281-286.
10. Doring S, Korhammer S, Oetken M, Markert B. 2000. Analysis of phytochelatins in plant matrices by pre-column derivatisation, high-performance liquid chromatography and fluorescence-detection. Fresenius J Anal Chem 366: 316-318.
11. Epstein E. 1972. Mineral Nutrition of Plants: Principles and Perspectives. Wiley: New York.
12. Fahey RC, Newton GL. 1987. Determination of low-molecular weight thiols using monobromobimane fluorescent labelling and high-performance liquid chromatography. Meth Enzymol 143: 85-97.
13. Fan TWM, Lane AN, Higashi RM. 1997. Selenium biotransformations by a euryhaline microalga isolated from a saline evaporation pond. Environ Sci Technol 31: 569-576.
14. Grill E, Gekeler W, Winnacker E-L, Zenk HH. 1986. Homophytochelatins are heavy metal-binding peptides of homo-glutathione containing Fabales. FEBS 205: 47-50.
15. Grill E, Winnacker EL, Zenk MH. 1991. Phytochelatins. Meth Enzymol 205: 333-341.
16. Hogstrand C, Haux C. 1992. Evaluation and differential pulse polarography for the quantification of metallothionein: A comparison with RIA. Anal Biochem 200: 388-392.
17. Kaegi JHR, Schaeffer A. 1988. Biochemistry of metallothionein. Biochemistry 27: 8509-8515.
18. Kägi JHR, Suzuki KT, Imura N, Kimura M. (eds). 1993. Metallothionein III: Biological Roles and Medical Implications. Birkhèauser: Basel; 29-55.
19. Klein D, Bartsch R, Summer KH. 1990. Quantitation of copper-containing metallothionein by a cadmium-saturation method. Anal Biochem 189: 35-39.
20. Kosower N, Kosower E. 1987. Thiol labelling with bromobimanes. Meth Enzymol 143: 76-84.
21. Kubota H, Sato K, Yamada T, Maitani T. 2000. Phytochelatin homologues induced in hairy roots of horseradish. Phytochemistry 53: 239-245.
22. Lazo JS, Pitt BR. 1995. Metallothioneins and cell death by anticancer drugs. A Rev Pharmacol Toxicol 35: 635-653.
23. Lobinski R, Szpunar J. 1999. Biochemical speciation analysis by hyphenated techniques. Anal Chim Acta 400: 321-332.
24. Lobinski R, Chassaigne H, Szpunar J. 1998. Analysis for metallothioneins using coupled techniques. Talanta 46: 271-289.
25. Maitani T, Kubota H, Sato K, Yamada T. 1996. The composition of metals bound to class III metallothionein (phytochelatin and its desglycyl peptide) induced by various metals in root cultures of Rubia tinctorum. Plant Physiol 110: 1145-1150.
26. Mehra RK, Winge DR. 1991. Metal ion resistance in fungi: molecular mechanisms and their regulated expression. J Cell Biochem 45: 30-40.
27. i-glutamyl peptides in Candida glabrata. Proc Natl Acad Sci USA 85: 8815-8819.
28. Meuwly P, Thibault P, Schwan AL, Rauser WE. 1995. Three families of thiol peptides are induced by cadmium in maize. Plant J 7: 391-400.
29. Miyairi S, Shibata, S, Naganuma, A. 1998. Determination of metallothionein by high-performance liquid chromatography with fluorescence detection using an isocratic solvent system. Anal Biochem 258: 168-175.
30. Mounicou S, Vacchina V, Szpunar J, Potin-Gautier M, Lobinski R. 2001. Determination of phytochelatins by capillary zone electrophoresis with electrospray tandem mass spectrometry detection (CZE-ES MS/MS). Analyst 126: 624-632.
31. Piotto M, Saudek V, Sklenar V. 1992. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J Biomol NMR: 661-665.
32. Prange A, Schaumloeffel D. 2002. Hyphenated techniques for the characterisation and quantification of metallothionein isoforms. Anal Bioanal Chem 373: 441-453.
33. Rauser WE. 1990. Phytochelatins. A Rev Biochem 59: 61-86.
34. Schagger H, von Jagow G. 1987. Tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368-379.
35. Speiser DM, Abrahamson SL, Banuelos G, Ow DW. 1992. Brassica juncea produces a phytochelatin-cadmium-sulphide complex. Plant Physiol 99: 817-821.
36. Suzuki K. 1998. Hyphenated techniques as tools for speciating biological metals: metallothionein and metal-binding proteins. Analusis 26: M57-64.
37. Vacchina V, Polec K, Szpunar J. 1999a. Speciation of cadmium in plant tissues by size-exclusion chromatography with ICP-MS detection. J Anal Atomic Spectrom 14: 1557-1566.
38. Vacchina V, Chassaigne H, Oven M, Zenk M, Lobinski R. 1999b. Characterisation and determination of phytochelatins in plant extracts by electrospray tandem mass spectrometry. Analyst 124: 1425-1430.
39. Yen T-Y, Villa JA, DeWitt JG. 1999. Analysis of phytochelatin-cadmium complexes from plant tissue culture using nano-electrospray ionisation tandem mass spectrometry and capillary liquid chromatography/electrospray ionisation tandem mass spectrometry. J Mass Spectrom 34: 930-941.
40. Zhou J, Goldsbrough PB. 1993. Arabidopsis metallothionein genes are functional in Saccharomyces cerevisiae. Plant Physiol 102: 30.