Inactivation of Helicobacter pylori cagA gene affects motility

Helicobacter

Volumn 9, Issue 3, 2004, Pages 185-193

  Figura, Natale ^a   Trabalzini, Lorenza ^b   Mini, Roberta ^b   Bernardini, Giulia ^b   Scaloni, Andrea ^c   Talamo, Fabio ^c   Lusini, Paola ^b   Ferro, Elisa ^b   Martelli, Paola ^b   Santucci, Annalisa ^b  

^a UNIVERSITY HOSPITAL OF SIENA   (Italy)

^b UNIVERSITY OF SIENA   (Italy)

^c CNR   (Italy)

Author keywords

CagA; Flagellin; Helicobacter pylori; Motility; Proteome; Two dimensional polyacrylamide gel electrophoresis

Indexed keywords

BACTERIAL ANTIGEN; CAGA PROTEIN; PROTEOME;

ARTICLE; BACTERIAL GENE; BACTERIAL MOTILITY; BACTERIAL STRAIN; BACTERIUM MUTANT; CAGA GENE; CELL MOTILITY; FLAGELLIN A GENE; FLAGELLIN B GENE; GENE DELETION; GENE DISRUPTION; GENE EXPRESSION; GENE INACTIVATION; GENETIC TRANSCRIPTION; HELICOBACTER PYLORI; HELICOBACTER PYLORI 328; HELICOBACTER PYLORI G27 DELTA CAGA; HELICOBACTER SPM328 DELTA CAGA; IMAGE ANALYSIS; MASS SPECTROMETRY; NONHUMAN; PATHOGENICITY; PATHOGENICITY ISLAND; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; RNA TRANSLATION; STRAIN DIFFERENCE; TWO DIMENSIONAL ELECTROPHORESIS;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; DYSPEPSIA; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLAGELLIN; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; MOVEMENT; PROTEOME;

BACTERIA (MICROORGANISMS); HELICOBACTER; HELICOBACTER PYLORI;

EID: 2642521054     PISSN: 10834389     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1083-4389.2004.00224.x     Document Type: Article

References (40)

1. Censini S, Lange C, Xiang Z, et al. cagA, a pathogenicity island of Helicobacter pylori, encodes type-I specific and disease-associated virulence factors. Proc Natl Acad Sci USA 1996;93:14648-53.
2. Stein M, Rappuoli R, Covacci A. Tyrosine phosphorylation of the Helicobacter pylori CagA antigen after cag-driven host cell translocation. Proc Natl Acad Sci USA 2000;97:1263-8.
3. Mimuro H, Suzuki T, Tanaka J, Asahi M, Haas R, Sasakawa C. Grb2 is a key mediator of Helicobacter pylori CagA protein activities. Mol Cell 2002;10:745-55.
4. Leying H, Suerbaum S, Geis G, Haas R. Cloning and genetic characterization of Helicobacter pylori flagellin gene. Mol Microbiol 1992;6:2863-74.
5. Suerbaum S, Josenhans C, Labigne A. Cloning and genetic characterization of the Helicobacter pylori and Helicobacter mustelae flaB flagellin genes and construction of H. pylori flaA- and flaB-negative mutants by electroporation-mediated allelic exchange. J Bacteriol 1993;175:3278-88.
6. Ottemann KM, Lowenthal AC. Helicobacter pylori uses motility for initial colonization and to attain robust infection. Infect Immun 2002;70:1984-90.
7. Yamaoka Y, Kodama T, Kashima K, Graham DY, Sepulveda AR. Variants of the 3, region of the caga gene in Helicobacter pylori isolates from patients with different H. pylori-associated diseases. J Clin Microbiol 1998;36:2258-63.
8. Atherton JC, Coa P Jr, Peek RM, Tummuru MKR, Blaser MJ, Cover TL. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. J Biol Chem 1995;270:17771-7.
9. Xiang Z, Censini S, Bayeli PF, et al. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. Infect Immun 1995;63:94-8.
10. Labigne A, Courcoux P, Tompkins L. Cloning of Campylobacter jejuni genes required for leucine biosynthesis, and construction of leu negative mutant of C. jejuni by shuttle transposon mutagenesis. Res Microbiol 1992;143:15-26.
11. Stein M, Bagnoli F, Halenbeck R, Rappuoli R, Fanti WJ, Covacci A. c-Src/Lyn kinases activate Helicobacter pylori CagA through tyrosine phosphorylation of the EPIYA motifs. Mol Microbiol 2002;43:971-80.
12. Copass M, Grandi G, Rappuoli R. Introducion of unmarked mutations in the Helicobacter pylori vaca gene with a sucrose sensitivity marker. Infect Immun 1997;65:1949-52.
13. Xiang Z, Bugnoli M, Ponzetto A, et al. Detection in an enzyme immunoassay of an immune response to a recombinant fragment of the 128 kilodalton protein of Helicobacter pylori. Eur J Clin Microbiol Infect Dis 1993;12:739-45.
14. Santucci A, Trabalzini L, Bovalini L, Neri P, Martelli P. Differences between genotypic and phenotypic sequences in Saccharomyces cerevisiae. Electrophoresis 2000;21:37171-23.
15. Towbin H, Stahelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4.
16. n telomeric repeated motif. Eur J Biochem 2001;268:139-48.
17. Josenhans C, Labigne A, Suerbaum S. Comparative ultrastructural and functional studies of Helicobacter pylori and Helicobacter mustelae flagellin mutants: both flagellin subunits, FlaA and FlaB, are necessary for full motility in Helicobacter species. J Bacteriol 1995;177:3010-20.
18. Kimmel B, Bosserhoff A, Frank R, Gross R, Goebel W, Beier D. Identification of immunodominant antigens from Helicobacter pylori and evaluation of their reactivities with sera from patients with different gastroduodenal pathologies. Infect Immun 2000;68:915-20.
19. Gygi SP, Rochon Y, Franza BR, Aebersold R. Correlation between protein and mRNA abundance in yeast. Mol Cell Biol 1999;19:1720-30.
20. Eaton KA, Suerbaum S, Josenhans C, Krakowka S. Colonization of gnotobiotic piglets by Helicobacter pylori deficient in two flagellin genes. Infect Immun 1996;64:2445-8.
21. Spohn G, Scarlato V. Motility of Helicobacter pylori is coordinately regulated by the transcriptional activator FlgR, an NtrC homolog. J Bacteriol 1999;181:593-9.
22. Lock RA, Coombs GW, McWilliams TM, et al. Proteome analysis of highly immunoreactive proteins of Helicobacter pylori. Helicobacter 2002;7:175-82.
23. Ge Z, Taylor DE. Contributions of genome sequencing to understanding the biology of Helicobacter pylori. Annu Rev Microbiol 1999;53:353-87.
24. Meyer-ter-Vehn T, Covacci A, Kist M, Pahl HL. Helicobacter pylori activates mitogen-activated protein kinase cascades and induces expression of the proto-oncogenes c-fos and c-jun. J Biol Chem 2000;275:16064-72.
25. Guillemin K, Salama NR, Tompkins LS, Falkow S. Cag pathogenicity island-specific responses of gastric epithelial cells to Helicobacter pylori infection. Proc Natl Acad Sci USA 2002;99:15136-41.
26. Hirata Y, Maeda S, Mitsuno Y, et al. Helicobacter pylori CagA protein activates serum response element-driven transcription independently of tyrosine phosphorylation. Gastroenterology 2002;123:1962-71.
27. Allan E, Dorrel N, Foynes S, Anyim M, Wren BW. Mutational analysis of genes encoding the early flagellar components of Helicobacter pylori: evidence for transcriptional regulation of flagellin A biosynthesis. J Bacteriol 2000;182:5274-7.
28. Jenks PJ, Foynes S, Ward SJ, Constantinidou C, Penn CW, Wren BW. A flagellar-specific ATPase (FliI) is necessary for flagellar export in Helicobacter pylori. FEMS Microbiol Lett 1997;152:205-11.
29. Schmitz A, Josenhans C, Suerbaum S. Cloning and characterization of the Helicobacter pylori flbA gene, which codes fora membrane protein involved in coordinated expression of flagellar genes. J Bacteriol 1997;179:987-97.
30. Bennet JC, Hughes C. From flagellum assembly to virulence: the extended family of type III export chaperones. Trends Microbiol 2000;8:202-4.
31. Rain JC, Selig L, de Reuse H, et al. The protein-protein interaction map of Helicobacter pylori. Nature 2001;409:211-15.
32. Kim JS, Chang JH, Chung SI, Yum JS. Molecular cloning and characterization of the Helicobacter pylori fliD gene, an essential factor in flagellar structure and motility. J Bacteriol 1999;181:6969-76.
33. Graham DY, Yamaoka Y. H. pylori and cagA. relationship with gastric cancer, duodenal ulcer and reflux esophagitis and its complications. Helicobacter 1998;3:145-51.
34. Marchetti M, Rappuoli R. Isogenic mutants of the cag pathogenicity island of Helicobacter pylori in the mouse model of infection: effects on colonization efficiency. Microbiology 2002;148:1447-56.
35. Wirth HP, Beins MH, Yang M, Tham KT, Blaser MJ. Experimental infection of Mongolian gerbils with wild-type and mutant Helicobacter pylori strains. Infect Immun 1998;66:4856-66.
36. Marchetti M, Arico B, Burroni D, Figura N, Rappuoli R, Ghiara P. Development of a mouse model of Helicobacter pylori infection that mimics human disease. Science 1995;267:1655-8.
37. Eaton KA, Morgan DR, Krakowka S. Motility as a factor in the colonisation of gnotobiotic piglets by Helicobacter pylori. J Med Microbiol 1992;37:123-7.
38. Young GM, Schmiel DH, Miller VL. A new pathway for the secretion of virulence factors by bacteria: the flagellar export apparatus functions as a protein-secretion system. Proc Natl Acad Sci USA 1999;96:6456-61.
39. McCarter L, Silverman M. Surface-induced swarmer cell differentiation of Vibrio parahaemolyticus. Mol Microbiol 1990;4:1057-62.
40. McGee DJ, May CA, Garner RM, Himpsl JM, Mobley HT. Isolation of Helicobacter pylori genes that modulate urease activity. J Bacteriol 1999;181:2477-84.