Sensitive detection using microfluidics technology of single cell PCR products from high and low abundance IgH VDJ templates in multiple myeloma

Journal of Immunological Methods

Volumn 305, Issue 1, 2005, Pages 94-105

  Pilarski, Linda M ^a,b   Lauzon, Jana ^a   Strachan, Erin ^a   Adamia, Sophia ^a   Atrazhev, Alexey ^a   Belch, Andrew R ^a   Backhouse, Christopher J ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b CROSS CANCER INSTITUTE   (Canada)

Author keywords

Cancer diagnostics; Detection of PCR product; Microfluidic chips; Novel technology

Indexed keywords

DNA FRAGMENT; GENOMIC DNA; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN HEAVY CHAIN VDJ; UNCLASSIFIED DRUG;

CAPILLARY ELECTROPHORESIS; CONFERENCE PAPER; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MICROFLUIDICS; MULTIPLE MYELOMA; PLASMA CELL; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS;

DNA, NEOPLASM; ELECTROPHORESIS, MICROCHIP; GENOME, HUMAN; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN HEAVY CHAINS; MULTIPLE MYELOMA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TEMPLATES, GENETIC; TRANSCRIPTION, GENETIC;

EID: 26244452159     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2005.07.010     Document Type: Conference Paper

References (18)

1. C.J. Backhouse, T. Footz, S. Adamia, and L.M. Pilarski Microfluidics chips for molecular analysis of human cancer Proceedings of the International Conference on MEMS, Nano and Smart Systems 2003 377
2. B. Barlogie, J. Epstein, P. Selvanayagam, and R. Alexanian Plasma cell myeloma-new biological insights and advances in therapy Blood 73 1989 865
3. D.E. Bergsagel Treatment of plasma cell myeloma Annu. Rev. Med. 30 1979 431
4. T. Footz, M.J. Somerville, R. Tomaszewski, K. Sprysak, and C.J. Backhouse Heteroduplex-based genotyping with microchip electrophoresis and dHPLC Genet. Test. 7 2003 283
5. T. Footz, M.J. Somerville, R. Tomaszewski, B. Elyas, and C.J. Backhouse Integration of combined heteroduplex/restriction fragment length polymorphism analysis on an electrophoresis microchip for the detection of hereditary haemochromatosis Analyst 129 2004 25
6. J. Kriangkum, B.J. Taylor, S.P. Treon, M.J. Mant, A.R. Belch, and L.M. Pilarski Clonotypic IgM V/D/J sequence analysis in Waldenstrom macroglobulinemia suggests an unusual B-cell origin and an expansion of polyclonal B cells in peripheral blood Blood 104 2004 2134
7. R. Ma, K.V.I.S. Kaler, and C.J. Backhouse A rapid performance assessment method for microfluidic chips W. Badawy W. Moussa International Conference on MEMS, NANO and Smart Systems (ICMENS) 2004 IEEE Computer Society Banff, AB
8. P.J. Obeid, T.K. Christopoulos, H.J. Crabtree, and C.J. Backhouse Microfabricated device for DNA and RNA amplification by continuous-flow polymerase chain reaction and reverse transcription-polymerase chain reaction with cycle number selection Anal. Chem. 75 2003 288
9. M. Palmer, A. Belch, J. Hanson, and L. Brox Reassessment of the relationship between M-protein decrement and survival in multiple myeloma Br. J. Cancer 59 1989 110
10. L.M. Pilarski, and A.R. Belch Intrinsic expression of the multidrug transporter, P-glycoprotein 170, in multiple myeloma; Implications for treatment Leuk. Lymphoma 17 1995 367
11. L.M. Pilarski, K. Seeberger, J.J. Keats, B.J. Taylor, R.W. Coupland, and A.R. Belch Leukemic B cells clonally identical to myeloma plasma cells are myelomagenic in NOD SCID mice Exp. Hematol. 30 2002 221
12. L.M. Pilarski, T. Reiman, P.M. Pilarski, F.W. Orr, and A.R. Belch The malignant hierarchy in multiple myeloma: relationships between malignant cells and bone disease G. Singh F.W. Orr Bone Metastasis and Molecular Mechanisms Pathophysiology 2004 Kluwer Academic Publishers The Netherlands 109
13. P.M. Pilarski, S. Adamia, and C.J. Backhouse An adaptable microvalving system for on-chip polymerase chain reactions J. Immunol. Methods 305 2005 48 10.1016/j.jim.2005.07.009
14. Q.M. Ralph, M.J. Brisco, D.E. Joshua, R. Brown, J. Gibson, and A.A. Morley Advancement of multiple myeloma from diagnosis through plateau phase to progression does not involve a new B-cell clone: evidence from the Ig heavy chain gene Blood 82 1993 202
15. T. Reiman, K. Seeberger, B.J. Taylor, A.J. Szczepek, J. Hansen, M.J. Mant, R.W. Coupland, A.R. Belch, and L.M. Pilarski Persistent pre-switch myeloma cells correlate with decreased survival: evidence for isotype switching within the myeloma clone Blood 98 2001 2791
16. A.J. Szczepek, K. Seeberger, J. Wizniak, M.J. Mant, A.R. Belch, and L.M. Pilarski A high frequency of circulating B cells share clonotypic IgH VDJ rearrangements with autologous bone marrow plasma cells in multiple myeloma, as measured by single cell and in situ RT-PCR Blood 92 1998 2844
17. G. Vahedi, K. Kaler, and C.J. Backhouse An integrated method for mutation detection using on-chip sample preparation, single stranded conformation polymorphism, and heteroduplex analysis Electrophoresis 25 2004 2346
18. Y. Zheng, T. Footz, D. Manage, and C.J. Backhouse Controlled and rapid self-assembly of DNA on a microfluidic chip J. Nanobiotechnol. 3 2 2005 http://www.jnanobiotechnology.com/articles/browse.asp?date=2-2005