Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry

World Journal of Gastroenterology

Volumn 11, Issue 33, 2005, Pages 5203-5208

  El Awady, Mostafa K ^a   Tabll, Ashraf A ^a   Redwan, El Rashdy M ^b   Youssef, Samar ^a   Omran, Moataza H ^a   Thakeb, Fouad ^c   El Demellawy, Maha ^b  

^a NATIONAL RESEARCH CENTRE   (Egypt)

^b Universities and Research centers District   (Egypt)

^c CAIRO UNIVERSITY   (Egypt)

Author keywords

Antibodies; Core; Envelope; Flow cytometry; Hepatitis C virus; Indirect immunofluorescence; Minus and plus RNA strand; Peripheral blood mononuclear cells

Indexed keywords

POLYCLONAL ANTIBODY; SYNTHETIC PEPTIDE; VIRUS ANTIGEN; VIRUS RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGEN DETECTION; ARTICLE; BLOOD SAMPLING; CELLULAR DISTRIBUTION; CONTROLLED STUDY; FLOW CYTOMETRY; GRANULOCYTE; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; INCUBATION TIME; LEUKOCYTE; LYMPHOCYTE; MONOCYTE; NONHUMAN; PROTEIN LOCALIZATION; STAINING; TEMPERATURE DEPENDENCE;

EID: 26244441764     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. World Health Organization. Global surveillance and control of hepatitis C. Report of a W.H.O. Consultation organized in collaboration with the Viral Hepatitis Prevention Board, Antwerp. Belgium J Viral Hepat 1999; 6: 35-47
2. Bartenschlager R, Lohmann V. Replication of hepatitis C virus. J Gen Virol 2000; 81: 1631-1648
3. Agnello V, Chung RT, Kaplan LM. A role for hepatitis C virus infection in type II cryoglobulinemia. N Engl J Med 1992; 327: 1490-1495
4. Quinn ER, Chan CH, Hadlock KG, Foung SH, Flint M. Levy S. The B cell-receptor of a hepatitis C virus (HCV)-associated non-Hodgkin lymphoma binds the viral E2 envelope protein, implicating HCV in lymphomagenesis. Blood 2001; 98: 3745-3749
5. Schmidt WN, Stapleton JT, LaBrecque DR, Mitros FA, Kirby PA, Phillips MJ, Brashear DL. Hepatitis C virus (HCV) infection and cryoglobulinemia: analysis of whole blood and plasma HCV-RNA concentrations and correlation with liver histology. Hepatol 2000; 31: 737-744
6. Wu E, Fernandez J, Fleck SK, Von Seggern DJ, Huang S. A 50-kDa membrane protein mediates sialic acid-independent binding and infection of conjunctival cells by adenovirus type 37. Virol 2001; 279: 78-89
7. Campadelli-Fiume G, Cocchi F, Menotti L, Lopez M. The novel receptors that mediate the entry of herpes simplex viruses and animal alphaherpesviruses into cells. Rev Med Virol 2000; 10: 305-319
8. Finnegan CM, Berg W, Lewis GK, DeVico AL. Antigenic properties of the human immunodeficiency virus envelope during cell-cell fusion. J Virol 2001; 75: 11096-11105
9. Klasse PJ, Bron R, Marsh M. Mechanisms of enveloped virus entry into animal cells. Adv. Drug Deliv Rev 1998; 34: 65-91
10. Pileri P, Uematsu Y, Campagnoli S, Galli G, Falugi F, Petracca R, Weiner AJ, Houghton M, Rosa D, Grandi G, Abrignani S. Binding of hepatitis C virus to CD81. Science 1998; 282: 938-941
11. Agnello V, Abel G, Elfahal M, Knight GB, Zhang QX. Hepatitis C virus and other Flaviviridae viruses enter cells via low density lipoprotein receptor. Proc Natl Acad Sci USA 1999; 96: 12766-12771
12. Monazahian M, Bohme I, Bonk S, Koch A, Scholz C, Grethe S, Thomssen R. Low density receptor as a candidate receptor for hepatitis C virus. J Med Virol 1999; 57: 223-229
13. Flint MC, Maidens LD, Loomis-Price C, Shotton J, Dubuisson P, Monk A, Higginbottom S, McKeating JA. Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81. J Virol 1999; 73: 6235-6244
14. Higginbottom A, Quinn ER, Kuo CC, Flint M, Wilson LH, Bianchi E, Nicosia A, Monk PN, McKeating JA, Levy S. Identification of amino acid residues in CD81 critical for interaction with hepatitis C virus envelope glycoprotein E2. J Virol 2000; 74: 3642-3649
15. Wünschmann S, Medh JD, Klinzmann D, Schmidt WN, Stapleton JT. Characterization of hepatitis C virus (HCV) and HCV E2 interactions with CD81 and the low-density lipoprotein receptor. J Virol 2000; 74: 10055-10062
16. Triyanti M, Saunier B, Maruvada P, Davis AR, Ulianich L. Interaction of hepatitis C virus-like particles and cells: a model system for studying viral binding and entry. J Virol 2002; 76: 9335-9344
17. Ohno O, Mizokami M, Wu RR, Saleh MG, Ohba K, Orito E, Mukaide M, Williams R, Lau JY. New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a and 6a. J Clinical Microbiol 1997; 35: 201-207
18. El Awady MK, El-Demellawy MA, Khalil SB, Galal D, Goueli SA. Synthetic peptide-based immunoassay as a supplemental test for HCV infection. Clin Chim Acta 2002; 325: 39-46
19. Hudson L, Hay FC. Antibody as a probe. In: Practical Immunology, third edition, Blackwell Scientific Publications Oxford London 1989: 34-43
20. Lohr HF, Goergen B. Buschenfelde KHMZ and Gerken G HCV replication in mononuclear cells stimulates anti-HCV secreting B cells and reflects non responsiveness to interferon A. J Med Virol 1995; 46: 314-320
21. Chomczynski P, Sacchi N. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162: 156-159
22. Crotty PL, Staggs RA, Porter PT, Killeen AA, McGlennen RC, Quantitative analysis in molecular diagnosis. Hum Pathol 1994; 25: 572-579
23. Reischl U, Kochanowski B. Quantitative PCR. A survey of the present technology. Mol Biotechnol 1995; 3: 55-71
24. Wang JT, Sheu JC, Lin JT, Wang TH, Chen DS. Detection of replication form of HCV RNA in peripheral blood mononuclear cells. J Infect Dis 1992; 166: 1167-1169
25. Taliani G, Badolato MC, Lecce R, Poliandri G, Bozza A, Duca F, Pasquazzi C, Clementi C, Furlan C, Bac CD. HCV RNA in PBMC: relation with response to IFN treatment. J Med Virol 1995; 47: 16-22
26. Zignego AL, De Carli M, Monti M, Careccia G, La Villa G, Giannini C, D'Elios MM, Del Prete G, Gentilini P. HCV infection of mononuclear cells from peripheral blood and liver infiltrates in chronically infected patients. J Med Virol 1995; 47: 58-64
27. Gribier B, Schmitt C, Bingen A, Kirn A, Keller F. In vitro infection of peripheral blood mononuclear cells by HCV. J Gen Virol 1995; 76: 2485-2491
28. Gong GZ, Lai LY, Jiang YF, He Y, Su XS. HCV replication in PBMC and its influence on interferon therapy. World J Gastroenterol 2003; 9: 291-294
29. El-Awady MK, Ismail SM, El-Sagheer M, Sabour YA, Amr KS, Zaki EA. Assay for hepatitis C virus in peripheral blood mononuclear cells enhances sensitivity of diagnosis and monitoring of HCV-associated hepatitis. Clin Chim Acta 1999; 283: 1-14
30. El-Awady MK, Abdel Rahman MM, Ismail SM, Amr KS, Omran M, Mohamed SA. Prediction of relapse after interferon therapy in hepatitis C virus-infected patients by the use of triple assay. Gastroenterol Hepatol 2003; 18: 68-73
31. Gabriellu A, Manzin A, Candela M. Active HCV infection in bone marrow and peripheral blood mononuclear cells from patients with mixed cryoglobulinaemia. Clin Exp Immunol 1994; 97: 87-93
32. Bouffard P, Hayashi PH, Acevedo R, Levy N, Zeldis JB. Hepatitis C virus is detected in a monocyte/macrophage subpopulation of peripheral blood mononuclear cells of infected patients. J Infect Dis 1992; 166: 1276-1280
33. Chang TT, Yong KC, Yang YJ, Lei HY, Wu HL. Hepatitis C virus RNA in PBMC: comparing acute and chronic hepatitis C virus infection. Hepatology 1996; 23: 977-981
34. Muler HM, Pfaff E, Goeser T, Kallinowski B, Solbach C. Peripheral blood leukocytes serve as a possible extrahepatic site for hepatitis C virus replication. J Gen Virol 1993; 74: 669-676
35. Mihm S, Hartmann H, Ramadori G. A reevaluation of the association of hepatitis C virus replicative intermediates with peripheral blood cells including granulocytes by a tagged reverse transcriptase/polymerase chain reaction technique. J Hepatol 1996; 24: 491-497
36. Lerat H, Berby F, Trabaud MA, Vidalin O, Major M, Trepo C, Inchauspe G. Specific detection of hepatitis C virus minus strand RNA in hematopoietic cells. J Clin Invest 1996; 97: 845-851
37. Sansonno D, Lacobelli AR, Cornacchiulo V, Iodice G, Dammacco F. Detection of HCV proteins by immunofluorescence and HCVRNA genomic sequences by non-isotopic in situ hybridization in bone marrow cells and PBMC of chronically HCV infected patients. Clin Exp Immunol 1996; 103: 414-421
38. Chen LB, Chen PL, Fan GR, Li L, Liu CY. Localization of hepatitis C virus core protein in the nucleus of PBMCs of hepatitis C patients. Zhonghua Shiyan He Linchuangbin Duxue Zazhi 2002; 16: 37-39